8-epi-PGF2α(8-Epi-Prostaglandin F2 Alpha) ELISA Kit
中文名称：8-异构前列腺素F2α(8-epi-PGF2α)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用8-epi-PGF2α抗原包被于酶标板上，实验时样品或标准品中的8-epi-PGF2α与包被的8-epi-PGF2α竞争生物素标记的抗8-epi-PGF2α单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，8-epi-PGF2α浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中8-epi-PGF2α的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的8-epi-PGF2α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Diagnostic value of 8-OHdG, 8-iso-PGF2α and TNF-α levels in blood for early carcinogenesis of erosive oral lichen planus
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Association between Serum 8-Iso-Prostaglandin F2α as an Oxidative Stress Marker and Immunological Markers in a Cohort of Preeclampsia Patients
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实验操作视频
