Elab Fluor® 647 Anti-Mouse F4/80 Antibody[CI:A3-1]
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100Tests
50Tests
100Tests×2
- 应用性: FCM
- 亚型: Rat IgG2b, κ
- 宿主: Rat
Background | F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance. |
Alternate Names | Adhesion G protein-coupled receptor E1;Adgre1;Cell surface glycoprotein F4/80;EGF-like module receptor 1;Adgre1;Emr1; Gpf480; |
Swissprot | |
Gene ID | |
Clone No | |
Host | Rat |
Reactivity | Mouse |
Application | |
Isotype | Rat IgG2b, κ |
Isotype Control | |
Conjugation | Elab Fluor®647 |
Conjugation Information | Elab Fluor® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter). |
Spectrum | |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA. |
Storage | This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze. |
Expiration date | 12个月 |
Shipping |
冰袋 |
Mouse abdominal macrophages elicited by starch broth are stained with Elab Fluor® 647 Anti-Mouse F4/80 Antibody (filled gray curve). Unstained macrophages (blank black curve) are used as control.
Q1:What are the recommendations for flow cytometry of mouse macrophages to detect M1 and M2 type macrophages?
As a recommandation, F4/80 and CD11b are always used for identyfing macrophages of mouse, and CD86 and CD206 can be used for a further seperation for M1 abd M2 respectively. Specific indicators can be determined according to the literature and experimental requirements.