Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
中文名称:谷丙转氨酶(ALT/GPT)比色法测试盒
Price:
Size:
100Assays(44 samples)
50Assays(19 samples)
- 样本类型: 血清(浆);动物组织;细胞
- 检测仪器及波长: 紫外-可见光分光光度计(505 nm)
- 研究领域: 肝肾功能
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量:
检测原理 |
在37℃、pH7.4条件下,谷丙转氨酶(ALT)催化丙氨酸与α-酮戊二酸之间的氨基转换反应,生成丙酮酸和谷氨酸,反应时间过后,加入苯肼,与丙酮酸生成苯腙,苯腙在碱性条件下呈红棕色。
|
指标简称 | ALT |
灵敏度 | 1.26 IU/L |
检测范围 | 1.26-72.3 IU/L |
精密度 | 批间差:8.2 | 批内差:4.3 |
检测时间 | 100 min |
检测仪器及波长 | 紫外-可见光分光光度计(505 nm) |
保存条件 | 该产品可在 2~8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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Q1:The tissue sample has been frozen for a long time, can it be used to detect AST/ALT?
It is recommended to use fresh samples as far as possible. If fresh samples are not available, it is recommended that the samples be frozen at -80℃ for no more than one month, otherwise the AST/ALT enzyme activity will decrease, which will have a great impact on the results.