ATP Chemiluminescence Assay Kit
中文名称:ATP含量化学发光法测试盒

Price:
- 样本类型: 动物组织;细胞
- 检测仪器及波长: 化学发光检测仪或多功能酶标仪(具有检测化学发光的功能)
- 研究领域: 氧化应激;三羧酸循环;线粒体功能;双硫死亡;能量代谢
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量:
检测原理 |
ATP与荧光素在荧光素酶的催化下,反应放出荧光,在一定范围内荧光强度与ATP浓度成正比关系。
|
灵敏度 | 0.003 μmol/L |
检测范围 | 0.003-5 μmol/L |
精密度 | 批间差:6.5% | 批内差:2.2% |
检测时间 | 30 min |
检测仪器及波长 | 化学发光检测仪或多功能酶标仪(具有检测化学发光的功能) |
保存条件 | 该产品可在-20°C避光条件下保存3个月。 |
有效期 | 3个月 |
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E-BC-K157-S | ATP Colorimetric Assay Kit | 100 Assays (48 samples) , 50 Assays (24 samples) |
Q1:试剂盒中的这个黑色酶标板可以重复使用吗?
代谢试剂盒中的酶标板都不可以重复使用。用过的孔就不能再用了,如果一次实验用不完所有的孔,可以将暂时不用的板孔用覆膜盖起来,避免污染,下次再用。如果这个试剂盒中酶标板不够用,可以用自己实验室新的黑色酶标板代替。
Q2:没有化学发光仪,可以使用普通的酶标仪吗?检测波长应该如何设置呢?
该试剂盒必须使用具备化学发光功能的仪器进行检测,没有化学发光仪,可以使用有化学发光功能的多功能酶标仪,不能使用普通酶标仪检测。该试剂盒检测时,只需要设置化学发光检测,不需要设置激发和发射波长。
Q3:我们的ATP试剂盒验证过大肠杆菌吗?能否检测大肠杆菌?
E-BC-F002试剂盒,实验室验证过大肠杆菌,可以检测。
Q4:ATP试剂盒中,试剂四存在絮状悬浮物,是正常现象吗?可以继续使用吗?
是正常现象,从-20℃拿出来后,放在室温解冻完就可以直接使用了。