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beta actin Monoclonal Antibody

  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
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  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
    产品货号:E-AB-20031

    别称:A26C1A,A26C1B,ACTB,ACTB,Actin beta,Actin cytoplasmic 1,Actin,cytoplasmic 1,N-terminally processed,Actx,b actin,Beta cytoskeletal actin,Beta-actin,BRWS1,E430023M04Rik,MGC128179,PS1TP5 binding protein 1,PS1TP5BP1

    规格:
    • 30μL
    • 60μL
    • 120μL
    • 300μL
    • 500μL
    数量:
    - +
    价格: ¥280

    宿主: Mouse

    反应性: H,M,R,Mk,Ch,Dg,Hm,Rb,Insect

    应用: WB,IHC-p

    Overview

    Synonyms A26C1A,A26C1B,ACTB,ACTB,Actin beta,Actin cytoplasmic 1,Actin,cytoplasmic 1,N-terminally processed,Actx,b actin,Beta cytoskeletal actin,Beta-actin,BRWS1,E430023M04Rik,MGC128179,PS1TP5 binding protein 1,PS1TP5BP1
    Swissprot P60709
    Source Mouse
    Reactivity Human,Mouse,Rat,Monkey,Chicken,Dog,Hamster,Rabbit,Insect
    Immunogen Synthetic Peptide
    Application WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R217)
    Recommended dilution WB 1:2000-1:5000, IHC 1:50-1:200
    Concentration 1mg/mL
    Clonality Monoclonal

    Properties

    Cellular localization Cytoplasm>cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    Clone No. Clone:2M3
    Isotype IgG
    Purification Protein A purification
    Conjugation Unconjugated
    Storage instructions Store at -20℃. Avoid freeze / thaw cycles.
    Storage buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.4
    Background Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins. 

    Images

    • Western Blot analysis of Hela, Rat brain, Mouse brain, Chicken lung, Rabbit testis using beta actin Monoclonal Antibody at dilution of 1:5000.

    • Immunohistochemistry of paraffin-embedded mouse kidney using beta actin Monoclonal Antibody at dilution of 1:200

    客户发表文献

    1. Dimethyl itaconate protects against fungal keratitis by activating the Nrf2/HO-1 signaling pathway
      IF: 3.745
      Journal: Immunology and cell biology(2020)
      DOI: 10.1111/imcb.12316
      PMID: 31943336
      引用产品: E-AB-1003 E-AB-20031 E-AB-20033 
      样本类型: Cell culture supernatant,Tissue homogenate
      物种: Human,Mouse
    2. Honokiol Reduces Fungal Load, Toll-Like Receptor-2, and Inflammatory Cytokines in Aspergillus fumigatus Keratitis
      IF: 3.47
      Journal: Investigative Ophthalmology & Visual Science(2020)
      DOI: 10.1167/iovs.61.4.48
      PMID: 32347916
      引用产品: E-AB-1021 E-AB-20031 E-AB-20082 E-EL-M0037c E-EL-M0049c E-EL-M0676c 
      样本类型: Cell culture supernatant
      物种: Mouse
    3. Perillaldehyde Ameliorates Aspergillus fumigatus Keratitis by Activating the Nrf2/HO-1 Signaling Pathway and Inhibiting Dectin-1-Mediated Inflammation
      IF: 3.47
      Journal: Investigative ophthalmology & visual science(2020)
      DOI: 10.1167/iovs.61.6.51
      PMID: 32579678
      引用产品: E-AB-20031 E-AB-20082 E-EL-H0102c E-EL-H0109c 
      样本类型: Cell lysate,Cell culture medium
      物种: Human
    4. Design and synthesis of C-19 isosteviol derivatives as potent and highly selective antiproliferative agents
      IF: 3.098
      Journal: Molecules(2019)
      DOI: 10.3390/molecules24010121
      PMID: 30598028
      引用产品: E-AB-20031 E-AB-31074 E-AB-62028 E-AB-70114 
      样本类型: Cell culture medium
      物种: Human
    5. HIF-1α-l-PGDS-PPARγ regulates hypoxia-induced ANP secretion in beating rat atria
      IF: 2.283
      Journal: Prostaglandins & other lipid mediators(2018)
      DOI: 10.1016/j.prostaglandins.2017.12.001
      PMID: 29287795
      引用产品: E-AB-20031 E-AB-70309 
      样本类型: Tissue homogenate
      物种: Rat
    6. Inhibition of lysyl oxidase expression by dextran sulfate affects invasion and migration of gastric cancer cells
      IF: 2.784
      Journal: International journal of molecular medicine(2018)
      DOI: 10.3892/ijmm.2018.3855
      PMID: 30226558
      引用产品: E-AB-20031 E-AB-30422 
      样本类型: Tissue homogenate
      物种: Human
    7. Tea polyphenols attenuate liver inflammation by modulating obesity-related genes and down-regulating COX-2 and iNOS expression in high fat-fed dogs
      IF: 1.835
      Journal: BMC Veterinary Research(2020)
      DOI: 10.1186/s12917-020-02448-7
      PMID: 32641048
      引用产品: E-AB-10495 E-AB-20031 E-AB-70031 
      样本类型: Serum,Tissue homogenate
      物种: Canine
    8. First Evidence Of Daam1 Localization In Mouse Seminal Vesicles And Its Possible Involvement During Regulated Exocytosis.
      IF: 1.1
      Journal: Comptes Rendus Biologies(2018)
      DOI: 10.1016/j.crvi.2018.03.001
      PMID: 29571963
      引用产品: E-AB-20031 
      物种: Mouse
    9. shRNA-Mediated Silencing Of PKHD1 Gene Promotes Proliferation, Migration And Invasion Of Human Intrahepatic Cholangiocarcinoma HuCCT-1 Cells.
      IF: 1.706
      Journal: International Journal of Clinical and Experimental Pathology(2017)
      引用产品: E-AB-20031 
    10. miR-516a-3p promotes proliferation, migration, and invasion and inhibits apoptosis in lung adenocarcinoma by targeting PTPRD
      IF: 0.252
      Journal: International Journal of Clinical and Experimental Pathology(2019)
      DOI: 99999999
      PMID: 31933822
      引用产品: E-AB-20031 
      样本类型: Tissue homogenate
      物种: Human
    11. Chemopreventive effect of hesperidin, a citrus bioflavonoid in two stage skin carcinogenesis in Swiss albino mice
      Journal: Heliyon(2019)
      DOI: 10.1016/j.heliyon.2019.e02521
      PMID: 31720442
      引用产品: E-AB-11525 E-AB-20031 E-AB-32280 E-AB-62000 E-AB-63496 
      样本类型: Tissue homogenate
      物种: Mouse
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