BRCA1 Polyclonal Antibody

Verified Samples in WB:293T,22RV1
Verified Samples in IHC:Human uterus cancer
Verified Samples in IF:Rat lung

WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000

Polyclonal

IgG

Synthesized peptide derived from human BRCA1 around the non-phosphorylation site of Ser1423.

BRCA1

BRCA 1;BRCA1;BRCA1 DNA repair associated;BRCA1/BRCA2 containing complex subunit 1;BRCA1/BRCA2-containing complex;subunit 1;BRCA1;BRCAI;BRCC 1;BRCC1;Breast and ovarian cancer susceptibility protein 1;Breast Cancer 1;Breast Cancer 1 Early Onset;Breast cancer type 1 susceptibility protein;BROVCA1;FANCS;IRIS;PNCA4;PPP1R53;Protein phosphatase 1 regulatory subunit 53;PSCP;RING finger protein 53;RNF53抗体

208kDa

Cytoplasm; Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs) and recruitment to DNA damage sites is mediated by the BRCA1-A complex.

1 mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Affinity purification

Cancer; Cell Biology; Epigenetics and Nuclear Signaling

Unconjugated

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.