Cell Copper (Cu) Colorimetric Assay Kit (Complexing Method)
中文名称:细胞铜(Cu)比色法测试盒(络合法)
Price:
- 样本类型: 细胞
- 检测仪器及波长: 酶标仪(575-585 nm)
- 研究领域: 无机盐离子,铜死亡
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量:
检测原理 |
在酸性条件下,铜离子从铜蓝蛋白上解离下来,与络合剂反应,产生紫色络合物,在一定范围内吸光度值与浓度成正比。
|
灵敏度 | 0.18 μmol/L |
检测范围 | 0.18-5 μmol/L |
精密度 | 批间差:3.1 | 批内差:3 |
检测时间 | 10 min |
检测仪器及波长 | 酶标仪(575-585 nm) |
保存条件 | 该产品可在 2~8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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Identification and development of a novel risk model based on cuproptosis-associated RNA methylation regulators for predicting prognosis and characterizing immune status in hepatocellular carcinoma
IF: 9.029Journal:Hepatology International
E-BC-K300-M | Copper (Cu) Colorimetric Assay Kit | 96T(80 samples) , 48T(32 samples) |
E-BC-K880-M | Cell Total Iron Colorimetric Assay Kit | 96T(80 samples) , 48T(32 samples) |
E-BC-K881-M | Cell Ferrous Iron Colorimetric Assay Kit | 96T(40 samples) , 48T(16 samples) |
Q1:Reagent 1 is a pink emulsion solid, can I use it?
This is a normal phenomenon, and the reagent 1 should be incubated at 37 ℃ until clear before use.
Q2:Does the cell sample need to be diluted?
If the copper ion content in the sample exceeds the maximum detection value of the kit, the sample needs to be diluted.However, because the copper ion content in cell samples is generally relatively low, this kit does not need to be diluted to detect cell samples.
Q3:Whether the kit detects Cu+ or Cu2+?
This kit measures the content of (Cu2+) in a sample.
Q4:What is the reason for the failure to detect the sample value with this kit?
It may be that the content of copper ions in the measured cells is low, and is lower than the detection limit of the kit. The number of sample cells can be appropriately increased to increase the concentration of copper ions in the detection sample.