Cell Ferrous Iron Colorimetric Assay Kit
中文名称：细胞亚铁比色法测试盒
Price:
- 样本类型： 细胞
- 检测仪器及波长： 酶标仪(590 - 600 nm，最佳检测波长为593 nm)
- 研究领域： 无机盐离子,铁死亡
生化试剂盒数量计算器x
需测样本数：
单个样本测量孔数：
试剂盒测样本孔数：
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量：
| 检测原理 |
样本中的亚铁离子与探针结合，生成的物质在593 nm波长处有强吸收峰，在一定范围内其光密度值与亚铁离子浓度成线性相关。
|
| 灵敏度 | 0.4 μmol/L |
| 检测范围 | 0.4-50 μmol/L |
| 精密度 | 批间差：1.5 | 批内差：1.3 |
| 检测时间 | 40 min |
| 检测仪器及波长 | 酶标仪(590 - 600 nm，最佳检测波长为593 nm) |
| 保存条件 | 该产品可在 2~8°C避光条件下保存6个月。 |
| 有效期 | 6个月 |
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| E-BC-K028-M | Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples) | 96T(92 samples) , 48T(44 samples) |
| E-BC-K030-M | Reduced Glutathione (GSH) Colorimetric Assay Kit | 96T(80 samples) , 48T(32 samples) |
| E-BC-K138-F | Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Green) | 96T |
| E-BC-K880-M | Cell Total Iron Colorimetric Assay Kit | 96T(80 samples) , 48T(32 samples) |
Q1:How is the cell sample broken?
The kit used the matching cell lysis buffer to process the cell samples. After adding the lysis buffer, it only needs to stand on the ice box for 10 minutes, then the supernatant can be centrifuged to be tested, which effectively improves the cell crushing efficiency.
Q2:What is the function of the control well mentioned in the manualof the E-BC-K881-M kit? Is a control well required for each sample?
The purpose of the control well setting is to exclude the interference of the sample itself. Each sample requires at least one control well and one sample well.
Q3:Can the final result be calculated not by cell number but by protein concentration?
The reagent 1 used in sample preparation will affect the determination of protein concentration, so it can not be calculated by protein concentration, but by cell number.
