Cell Staining Buffer

Q1:What is the function of cell staining buffer? Can I use PBS ? How much buffer does a sample need?

Cell staining buffer is mainly to maintain cell vitality, keep cells stable from damagimg and operating, maximize the intensity of the fluorescence signal of ph-sensitive fluorescent dyes, reduce the non-specific binding of antibodies to target cells, chelate metal cations to reduce adhesion between cells. Cell staining buffer might be replaced by PBS solution of 1%BSA. About 1.5 mL of cell stain buffer is required for each sample.