EGFR Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
- 宿主: Rabbit
- 反应性: Human
- 应用性: WB
Verified Samples |
Verified Samples in WB:A549,HepG2,MCF-7,HCT116 |
Dilution | WB 1:500-1:2000 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse EGFR |
Abbre | EGFR |
Synonyms | Avian erythroblastic leukemia viral (v erb b) oncogene homolog;Cell growth inhibiting protein 40;Cell proliferation inducing protein 61;EGF R;EGFR;EGFR;Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog);Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian);Epidermal growth factor receptor;erb-b2 receptor tyrosine kinase 1;ERBB;ERBB1;Errp;HER1;mENA;NISBD2;Oncogen ERBB;PIG61;Proto-oncogene c-ErbB-1;Receptor tyrosine protein kinase ErbB 1;Receptor tyrosine-protein kinase ErbB-1;SA7;Species antigen 7;Urogastrone;v-erb-b Avian erythroblastic leukemia viral oncogen homolog;wa2;Wa5抗体 |
Swissprot | |
Calculated MW | 134kDa |
Observed MW | 175kDa |
Cellular Localization | Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF). |
Concentration | 110 μg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer; Signal Transduction |
Conjugation | Unconjugated |
Background | EGFR,also named as ERBB1,is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12,encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression,including cell proliferation,apoptosis,angiogenesis,and metastatic spread. Mutations in this gene are associated with lung cancer. |
Western Blot analysis of various samples using EGFR Polyclonal Antibody at dilution of 1:750.
E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
E-IR-R304A | Western Blot Detection Kit | 50Assays |
E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |