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Elab Fluor^® Violet 450 Anti-Mouse CD22 Antibody[Cy34.1]

Uniprot : P35329

货号: E-AB-F1021UQ
应用: FCM
Isotype: Mouse IgG1, κ
宿主: Mouse

Applications:

说明书

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规格:

25μg
100μg

价格: ￥1080

数量:

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Antigen Details

Alternate Names	B-cell receptor CD22,Cd22,B-lymphocyte cell adhesion molecule,BL-CAM,Sialic acid-binding Ig-like lectin 2,Siglec-2,T-cell surface antigen Leu-14,CD22,Lyb-8, Siglec2
Swissprot	P35329
Gene ID	12483
References	1. Symington FW et al. 1982. Immunogenetics 16:381. 2. Erickson LD et al. 1996. Int Immunol 8:1121. 3. Doody GM et al. 1995. Science 269:242.

Product Details

Form	Liquid
Clone No.	Cy34.1 (See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	Elab Fluor^® Violet 450 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09793Q]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® Violet 450
	Elab Fluor^® Violet 450 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450 nm (e.g., a 450/45 nm bandpass filter).

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Background

The Cy34.1 monoclonal antibody specifically binds to the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g., A, BALB/c, CBA, C3H/He, C57BL, C57L, C58, SJL, SWR, but not AKR, DBA/1, DBA/2, NZB, PL). CD22 is expressed at high levels on mature peripheral B lymphocytes (follicular and marginal zone), B-1 cells (CD5+ B cells), and plasma cells. It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. Its intracellular domain is phosphorylated after cross-linking of antigen receptor or MHC class II antigen. It is involved in negative regulation of B-cell activation and protection from autoimmunity. B-cell proliferative responses to LPS or anti-mouse Ig µ chain are augmented in the presence of Cy34.1 mAb.

Protocols & Troubleshootings

Related Protocols	流式细胞表面染色步骤
Troubleshooting	流式实验常见问题及解决方案

Alternative Formats

Cat.No.	Product Name	Sizes
E-AB-F10210	AF/LE Purified Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F10210	50μg, 500μg, 1mg
E-AB-F1021A	Purified Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021A	25μg, 100μg
E-AB-F1021B	Biotin Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021B	25μg, 100μg
E-AB-F1021E	APC Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021E	50Tests, 100Tests, 100Tests×2
E-AB-F1021G	PE/Cyanine5 Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021G	50Tests, 100Tests, 100Tests×2
E-AB-F1021H	PE/Cyanine7 Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021H	50Tests, 100Tests, 100Tests×2
E-AB-F1021I	PE/Cyanine5.5 Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021I	50Tests, 100Tests, 100Tests×2
E-AB-F1021J	PerCP/Cyanine5.5 Anti-Mouse CD22 Antibody[Cy34.1]	50Tests, 100Tests, 100Tests×2
E-AB-F1021L	Elab Fluor^® 488 Anti-Mouse CD22 Antibody[Cy34.1]	50Tests, 100Tests, 100Tests×2
E-AB-F1021M	Elab Fluor^® 647 Anti-Mouse CD22 Antibody[Cy34.1]	50Tests, 100Tests, 100Tests×2
E-AB-F1021Q	Elab Fluor^® Violet 450 Anti-Mouse CD22 Antibody[Cy34.1]	50Tests, 100Tests, 100Tests×2
E-AB-F1021UE	APC Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021UE	25μg, 100μg
E-AB-F1021UG	PE/Cyanine5 Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021UG	25μg, 100μg
E-AB-F1021UH	PE/Cyanine7 Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021UH	25μg, 100μg
E-AB-F1021UI	PE/Cyanine5.5 Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021UI	25μg, 100μg
E-AB-F1021UJ	PerCP/Cyanine5.5 Anti-Mouse CD22 Antibody[Cy34.1]	25μg, 100μg
E-AB-F1021UL	Elab Fluor^® 488 Anti-Mouse CD22 Antibody[Cy34.1]	25μg, 100μg
E-AB-F1021UM	Elab Fluor^® 647 Anti-Mouse CD22 Antibody[Cy34.1]	25μg, 100μg