Elab Fluor® 488-conjugated GAPDH Monoclonal Antibody

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- 宿主: Mouse
- 反应性: Human;Mouse
- 应用性: IF
Verified Samples |
Verified Samples in IF: Hela |
Dilution | IF 1:100-1:200 |
Clonality | Monoclonal |
Isotype | IgG1 |
Immunogen | Recombinant human GAPDH protein expressed by E.coli |
Abbre | GAPDH |
Synonyms | BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, aging associated gene 9 protein, cb609, p38 component |
Swissprot | |
Cellular Localization | Cytoplasm>cytosol, Nucleus, Cytoplasm>perinuclear region, Membrane, Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity), Postnuclear and Perinuclear regions. |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
Purification Method | Protein A/G Purification |
Research Areas | Signal Transduction, Metabolism, Cancer, Neuroscience, Isotype/Loading Controls, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers, Biochemicals, Cardiovascular, Developmental Biology |
Clone No. | 7B8 |
Conjugation | Elab Fluor®488 |
Amax | 493nm |
Emax | 519nm |
Background | GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells,it is commonly used as a loading control for western blot and as a control for qPCR. |