Enhanced Cell Counting Kit 8 (WST-8/CCK8)
中文名称：增强型细胞活力检测试剂盒(CCK-8)
Price:
- 应用： 细胞活力/细胞增殖/细胞毒性
| 检测原理 | WST-8 (2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐)，在电子载体存在的情况下，WST-8被线粒体内的脱氢酶还原生成水溶性的橙黄色formazan，生成的formazan的量与活细胞数量成正比，通过检测在450 nm处的吸光度，可以间接计算活细胞的量。 |
| 检测方法 | 比色法 |
| 样本类型 | 细胞 |
| 检测仪器 | 酶标仪 |
| 保存温度 | 该产品可在 2~8°C或-20°C避光条件下保存24个月。 |
| 有效期 | 24个月 |
| 运输条件 | 冰袋 |
-
CBX2 shapes chromatin accessibility promoting AML via p38 MAPK signaling pathway
IF: 41.444 -
HTR2A agonists play a therapeutic role by restricting ILC2 activation in papain-induced lung inflammation
IF: 22.096 -
Polystyrene nanoparticle exposure supports ROS-NLRP3 axis-dependent DNA-NET to promote liver inflammation
IF: 14.224 -
Melatonin alleviates the heat stress-induced impairment of Sertoli cells by reprogramming glucose metabolism
IF: 12.081 -
Controllable graphene oxide-based biocompatible hybrid interface as an anti-fibrotic coating for metallic implants
IF: 10.761 -
AQP2 Promotes Astrocyte Activation by Modulating the TLR4/NFκB-p65 Pathway Following Intracerebral Hemorrhage.
IF: 8.786Journal：Frontiers in Immunology -
Identification and validation of a prognostic signature of autophagy, apoptosis and pyroptosis-related genes for head and neck squamous cell carcinoma: to imply therapeutic choices of HPV negative patients
IF: 8.786Journal：Frontiers in Immunology -
Efficacy of WWQ-131, a highly selective JAK2 inhibitor, in mouse models of myeloproliferative neoplasms
IF: 7.419 -
Cascade targeting codelivery of ingenol-3-angelate and doxorubicin for enhancing cancer chemoimmunotherapy through synergistic effects in prostate cancer
IF: 7.348Journal：Materials Today Bio -
Luteolin ameliorates hypoxia-induced pulmonary hypertension via regulating HIF-2α-Arg-NO axis and PI3K-AKT-eNOS-NO signaling pathway
IF: 6.656
| E-CK-A211 | Annexin V-FITC/PI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
| E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100Assays , 50Assays , 20Assays |
| E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100Assays , 50Assays , 20Assays |
| E-CK-A341 | MTT Cell Proliferation and Cytotoxicity Assay Kit | 1000Assays , 500Assays |
Q1:Will the color of drug that used to stimulate cells affect the results of CCK-8 experiment?
The effect of drug color can be eliminated by changing the medium after the drug incubation and before adding CCK-8.
Q2:What are the differences among MTS, XXT, WST-1, CCK-8 (WST-8) test kits?
MTT assay kits are widely used to detect cell proliferation and cytotoxicity, and MTS, XXT, WST-1 and WST-8 are all upgraded alternatives to MTT, which have obvious advantages compared with MTT. On the one hand, the reaction production of MTT assay is insoluable in water, a specific solvent is required in the assay, products of WST-8, XTT and MTS are water-soluble with easier operation. On the other hand, the solubility of the formazan produced of WST-8 is higher than that of XTT and MTS. What's more, WST-8 is more stable than MTT, XTT, and has a wider linear range and higher sensitivity.
