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EPI(Epinephrine/Adrenaline) ELISA Kit

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    产品货号:E-EL-0045c

    中文名:肾上腺素(EPI)酶联免疫吸附测定试剂盒

    别称:Epinephrine, adrenaline, adrenalin, 4, 5-β-trihydroxy-N-methylphenethylamine

    规格:
    • 96T
    • 48T
    • 96T*5
    数量:
    - +
    价格: ¥2400

    反应性: 通用

    检测范围: 31.25~2000 pg/mL

    灵敏度: 18.75 pg/mL

    用途

    该试剂盒用于体外定量检测血清、血浆或其他相关生物液体中EPI浓度。

    检测原理

    本试剂盒采用竞争ELISA法。用EPI抗原包被于酶标板上,实验时样品或标准品中的EPI与包被的EPI竞争生物素标记的抗EPI单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,EPI浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中EPI的浓度。

    反应类型 竞争法
    规格 96T
    反应时间 2.0h
    反应性 通用
    检测方法 Colormetric
    检测范围 31.25-2000 pg/mL
    灵敏度 18.75 pg/mL
    样本体积 50μL
    样本类型 血清、血浆或其他相关生物液体

    特异性

    可检测样本中的EPI,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average
    2000 0.317
    0.377
    0.347
    1000 0.44
    0.498
    0.469
    500 0.683
    0.681
    0.682
    250 1.006
    1.02
    1.013
    125 1.446
    1.438
    1.442
    62.5 1.885
    1.881
    1.883
    31.25 2.239
    2.243
    2.241
    0 2.771
    2.781
    2.776

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

      Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    96.50 321.00 901.00 88.20 307.60 931.00
    Standard
    deviation
    6.30 13.50 36.00 4.90 13.50 37.20
    C V (%) 6.53 4.21 4.00 5.56 4.39 4.00

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=8) 88-101 93
    EDTA plasma (n=8) 95-109 101
    Cell culture media (n=8) 89-100 95

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

        Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 91-102 86-99 100-111
    Average (%) 97 91 105
    1:4 Range (%) 85-97 91-102 99-113
    Average (%) 90 96 106
    1:8 Range (%) 85-100 88-102 99-113
    Average (%) 92 95 106
    1:16 Range (%) 88-102 90-102 96-111
    Average (%) 96 96 103

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 2-8℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 2-8℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 2-8℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1.酶标仪(450nm波长滤光片)
    • 2.高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3.37℃恒温箱,双蒸水或去离子水
    • 4.吸水纸

    操作步骤

    1. 对应板孔中加入50μL标准品工作液或样本后,立即每孔加入50μL生物素化抗体工作液,37℃孵育45分钟

    2. 弃掉板内液体,洗板3次

    3. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次

    4. 每孔加入90μL底物溶液,37℃孵育15分钟左右

    5. 每孔加入50μL终止液

    6. 立即在450nm波长下读数,处理数据

    客户发表文献

    1. Light modulates glucose metabolism by a retina-hypothalamus-brown adipose tissue axis
      IF: 66.849
      Journal: CELL(2023)
      DOI: 10.1016/j.cell.2022.12.024
      PMID: 36669474
      样本类型: Serum
      物种: Mouse
    2. β-adrenoreceptor-triggered PKA activation negatively regulates the innate antiviral response
      IF: 22.096
      Journal: Cellular & Molecular Immunology(2023)
      DOI: 10.1038/s41423-022-00967-x
      PMID: 36600052
      样本类型: serum
      物种: Mouse
    3. Neuroendocrine stress hormones associated with short-term exposure to nitrogen dioxide and fine particulate matter in individuals with and without chronic obstructive pulmonary disease: A panel study in Beijing, China
      IF: 9.988
      Journal: ENVIRONMENTAL POLLUTION(2022)
      DOI: 10.1016/j.envpol.2022.119822
      PMID: 35870527
      样本类型: serum
      物种: Human
    4. Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors
      IF: 6.018
      Journal: Computational and Structural Biotechnology Journal(2020)
      DOI: 10.1016/j.csbj.2020.11.009
      PMID: 33304464
      样本类型: blood
      物种: Human
    5. Metformin prevents the pathological browning of subcutaneous white adipose tissue
      IF: 6.181
      Journal: Molecular Metabolism(2019)
      DOI: 10.1016/j.molmet.2019.08.011
      样本类型: plasma
      物种: Human
    6. Maternal anxiety affects embryo implantation via impairing adrenergic receptor signaling in decidual cells
      IF: 6.548
      Journal: Communications Biology(2022)
      DOI: 10.1038/s42003-022-03694-1
      PMID: 35982177
      样本类型: serum,uteri
      物种: Mouse
    7. Uncoupling protein -1 modulates anxiety-like behavior in a temperature-dependent manner
      IF: 6.709
      Journal: JOURNAL OF NEUROSCIENCE(2022)
      DOI: 10.1523/JNEUROSCI.2509-21.2022
      PMID: 36194650
      样本类型: serum
      物种: Mouse
    8. Caffeine supplementation induces higher IL-6 and IL-10 plasma levels in response to a treadmill exercise test
      IF: 5.068
      Journal: Journal of the International Society of Sports Nutrition(2020)
      DOI: 10.1186/s12970-020-00375-4
      PMID: 32907591
      样本类型: Plasma
      物种: Human
    9. Protective effect of low-dose radiation on doxorubicin-induced brain injury in mice
      IF: 4.114
      Journal: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS(2022)
      DOI: 10.1016/j.abb.2022.109390
      PMID: 36067878
      样本类型: brain tissue
      物种: Mouse
    10. Beneficial Effects of lncRNA-UC.360+ shRNA on Diabetic Cardiac Sympathetic Damage via NLRP3 Inflammasome-Induced Pyroptosis in Stellate Ganglion
      IF: 4.132
      Journal: ACS Omega(2022)
      DOI: 10.1021/acsomega.2c03619
      PMID: 35967043
      样本类型: serum
      物种: Rat
    11. Deep transcranial magnetic stimulation in combination with skin thermography in obesity: a window on sympathetic nervous system
      IF: 4.28
      Journal: ACTA DIABETOLOGICA(2022)
      DOI: 10.1007/s00592-022-01859-2
      PMID: 35174415
      样本类型: blood
      物种: Human
    12. Investigating the molecular mechanisms of delirium-like neuropsychiatric disorder induced by electromagnetic pulse based on bioinformatics analysis
      IF: 4.399
      Journal: Molecular Brain(2023)
      DOI: 10.1186/s13041-023-00998-z
      样本类型: prefrontal cortex,hippocampus
      物种: Rat
    13. High frequency deep transcranial magnetic stimulation acutely increases β-endorphins in obese humans
      IF: 3.179
      Journal: ENDOCRINE(2018)
      DOI: 10.1007/s12020-018-1791-1
      样本类型: blood
      物种: Human
    14. Intranasal Dexmedetomidine on Stress Hormones, Inflammatory Markers, and Postoperative Analgesia after Functional Endoscopic Sinus Surgery
      IF: 3.236
      Journal: MEDIATORS OF INFLAMMATION(2015)
      DOI: 10.1155/2015/939431
      样本类型: Serum
      物种: Human
    15. Perivascular radiofrequency renal denervation lowers blood pressure and ameliorates cardiorenal fibrosis in spontaneously hypertensive rats
      IF: 3.24
      Journal: PLoS One(2017)
      DOI: 10.1371/journal.pone.0176888
      样本类型: plasma
      物种: Rat
    16. A Case Control Study on Serum Levels of Potential Biomarkers in Male Breast Cancer Patients
      IF: 3.39
      Journal: International Journal of Environmental Research and Public Health(2021)
      DOI: 10.3390/ijerph18094852
      PMID: 34062830
      样本类型: Serum
      物种: Human
    17. Primary stress response and biochemical profile of Labeo rohita (Hamilton, 1822) experimentally parasitized with Argulus bengalensis (Ramakrishna, 1951)
      IF: 2.051
      Journal: JOURNAL OF FISH BIOLOGY(2022)
      DOI: 10.1111/jfb.15048
      PMID: 35319798
      样本类型: plasma
      物种: Fish
    18. LianXia Formula Granule Attenuates Cardiac Sympathetic Remodeling in Rats with Myocardial Infarction via the NGF/TrKA/PI3K/AKT Signaling Pathway
      IF: 2.63
      Journal: Evidence-based Complementary and Alternative Medicine(2021)
      DOI: 10.1155/2021/5536406
      PMID: 34221073
      样本类型: plasma
      物种: Rat
    19. A Novel Swine Model of Spontaneous Hypertension With Sympathetic Hyperactivity Responds Well to Renal Denervation
      IF: 2.689
      Journal: AMERICAN JOURNAL OF HYPERTENSION(2016)
      DOI: 10.1093/ajh/hpv066
      样本类型: Plasma,Urine
      物种: Pig
    20. Selective ablation of the ligament of Marshall reduces ischemia and reperfusion-induced ventricular arrhythmias
      IF: 2.766
      Journal: PLoS One(2018)
      DOI: 10.1371/journal.pone.0203083
      PMID: 30153281
      样本类型: Serum
      物种: Canine
    21. Stress response to ovariohysterectomy in rabbits: role of anaesthesia and surgery
      IF: 0.545
      Journal: JOURNAL OF OBSTETRICS AND GYNAECOLOGY(2018)
      DOI: 10.1080/01443615.2017.1400523
      样本类型: Serum,Plasma
      物种: Rabbit
    22. Effects of stocking density on stress responses and meat quality characteristics of lambs transported for 45 minutes or 3 hours
      IF: 0.974
      Journal: SMALL RUMINANT RESEARCH(2018)
      DOI: 10.1016/j.smallrumres.2018.08.009
      样本类型: serum
      物种: Sheep
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