FITC Anti-Human CD206/MMR Antibody[15-2]
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- 应用性： FCM
- 亚型： Mouse IgG1, κ
- 宿主： Mouse
| Background | Macrophage mannose receptor (MMR) is a 162-175 kD type I membrane protein also known as CD206, MRC1, or mannose receptor (MR). It is a pattern recognition receptor (PRR) that belongs to C-type lectin superfamily. MMR is expressed on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes. MMR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine. MMR mediates endocytosis and phagocytosis, induces activation of macrophages and antigen presentation, plays an important role in host defense, and provides a link between innate and adaptive immunity. |
| Alternate Names | CLEC13D;CLEC13DL;MRC1L1;MMR;hMR; |
| Swissprot | |
| Gene ID | |
| Clone No | |
| Host | Mouse |
| Reactivity | Human |
| Application | |
| Isotype | Mouse IgG1, κ |
| Isotype Control | |
| Conjugation | FITC |
| Conjugation Information | FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter). |
| Spectrum |  |
| Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA. |
| Storage | This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze. |
| Expiration date | 12个月 |
| Shipping |
冰袋 |
| E-AB-F1161E | APC Anti-Human CD206/MMR Antibody[15-2] | 100Tests , 20Tests , 100Tests×2 |
Q1:Is it necessary to take fixation/permeabilization operation while detecting CD206?
CD206 is a transmembrane protein, which can be expressed on both side of the cell membrane. With or without permeabilization are both avaliable for CD206 detection as our anti-mouse CD206 flow antibody can bind both side of CD206. Generally it is recommanded to take fixation/permeabilization operation but these two alternative processes can be decided through the experimental design.
Q2:What are the recommendations for flow cytometry of mouse macrophages to detect M1 and M2 type macrophages?
As a recommandation, F4/80 and CD11b are always used for identyfing macrophages of mouse, and CD86 and CD206 can be used for a further seperation for M1 abd M2 respectively. Specific indicators can be determined according to the literature and experimental requirements.
