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FITC Anti-Mouse F4/80 Antibody[CI:A3-1]

Price: ¥576 ¥336 ¥864

Size:
100Tests 50Tests 100Tests×2
  • 应用性: FCM
  • 亚型: Rat IgG2b, κ
  • 宿主: Rat
Background
F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.
Alternate Names
Adhesion G protein-coupled receptor E1;Adgre1;Cell surface glycoprotein F4/80;EGF-like module receptor 1;Adgre1;Emr1; Gpf480;
Swissprot
Gene ID
Clone No
Host
Rat
Reactivity
Mouse
Application
Isotype
Rat IgG2b, κ
Isotype Control
Conjugation
FITC
Conjugation Information
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
Spectrum
Storage Buffer
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
Storage
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Expiration date
12个月
Shipping
冰袋
C57BL/6 murine abdominal macrophages are stained with FITC Anti-Mouse F4/80 Antibody (filled gray histogram). Unstained abdominal macrophages (empty black histogram) are used as control.
E-AB-F09950 AF/LE Purified Anti-Mouse F4/80 Antibody[CI:A3-1] 500μg , 50μg , 1mg
E-AB-F0995A Purified Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg
E-AB-F0995B Biotin Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg
E-AB-F0995D PE Anti-Mouse F4/80 Antibody[CI:A3-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0995E APC Anti-Mouse F4/80 Antibody[CI:A3-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0995G PE/Cyanine5 Anti-Mouse F4/80 Antibody[CI:A3-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0995H PE/Cyanine7 Anti-Mouse F4/80 Antibody[CI:A3-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0995I PE/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0995J PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0995L Elab Fluor® 488 Anti-Mouse F4/80 Antibody[CI:A3-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0995M Elab Fluor® 647 Anti-Mouse F4/80 Antibody[CI:A3-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0995P PE/Elab Fluor® 594 Anti-Mouse F4/80 Antibody[CI:A3-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0995Q Elab Fluor® Violet 450 Anti-Mouse F4/80 Antibody[CI:A3-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0995S Elab Fluor® Red 780 Anti-Mouse F4/80 Antibody[CI:A3-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0995UC FITC Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg
E-AB-F0995UD PE Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg
E-AB-F0995UE APC Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg
E-AB-F0995UG PE/Cyanine5 Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg
E-AB-F0995UH PE/Cyanine7 Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg
E-AB-F0995UI PE/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg
E-AB-F0995UJ PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg
E-AB-F0995UL Elab Fluor® 488 Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg
E-AB-F0995UM Elab Fluor® 647 Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg
E-AB-F0995UP PE/Elab Fluor® 594 Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg
E-AB-F0995UQ Elab Fluor® Violet 450 Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg
E-AB-F0995US Elab Fluor® Red 780 Anti-Mouse F4/80 Antibody[CI:A3-1] 100μg , 25μg

Q1:What are the recommendations for flow cytometry of mouse macrophages to detect M1 and M2 type macrophages?

As a recommandation, F4/80 and CD11b are always used for identyfing macrophages of mouse, and CD86 and CD206 can be used for a further seperation for M1 abd M2 respectively. Specific indicators can be determined according to the literature and experimental requirements.



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