FITC-conjugated Flag-Tag Monoclonal Antibody

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- 宿主: Mouse
- 反应性: All
- 应用性: FCM
Verified Samples |
Verified Samples in FCM: 293F cells were transfected with a tag expression plasmid to prepare an overexpression cell model |
Dilution | FCM 0.2 μg per 10^6 cells in a 100 μL suspension |
Clonality | Recombinant |
Isotype | IgG2a |
Immunogen | DYKDDDDK tag fusion protein |
Abbre | Flag Tag |
Synonyms | DDDDK epitope tag, DYKDDDDK epitope tag |
Concentration | 1 mg/mL |
Storage | Store at 4°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
Buffer | PBS with 0.05% proclin 300 and 0.1% gelatin,pH7.4 |
Purification Method | Protein A/G Purification |
Research Areas | Tags and Cell Markers, Microbiology, Immunology, Neuroscience |
Clone No. | 3H9 |
Conjugation | FITC |
Amax | 495nm |
Emax | 525nm |
Background | FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology,having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications. |