High Sensitivity Human IFN-γ (Interferon Gamma) ELISA Kit
中文名称:高敏型人γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 1.56-100 pg/mL
- 灵敏度: 0.98 pg/mL
系列介绍 | Elabscience®采用迭代升级新工艺,自主研发的高敏(High Sensitivity)ELISA试剂盒,聚焦于细胞因子检测,与传统ELISA试剂盒相比,灵敏度提升了2-10倍,可检测fg级别的蛋白浓度,有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IFN-γ浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFN-γ抗体包被于酶标板上,实验时样品(或标准品)中的人IFN-γ会与包被抗体结合。后依次加入生物素化的抗人IFN-γ抗体和辣根过氧化物酶标记的亲和素,抗人IFN-γ抗体与结合在包被抗体上的人IFN-γ结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IFN-γ浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 1.56-100 pg/mL |
灵敏度 | 0.98 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人IFN-γ,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Extracellular vesicle-packaged lncRNA from cancer-associated fibroblasts promotes immune evasion by downregulating HLA-A in pancreatic cancer
IF: 15.500 -
Sex-specific circulating unconventional neutrophils determine immunological outcome of auto-inflammatory Beh?et’s uveitis
IF: 13.000 -
Tumor-associated macrophage enhances PD-L1-mediated immune escape of bladder cancer through PKM2 dimer-STAT3 complex nuclear translocation
IF: 9.100 -
Ginsenoside Rg3 activates the immune function of CD8+?T cells via circFOXP1-miR-4477a-PD-L1 axis to induce ferroptosis in gallbladder cancer
IF: 6.900 -
Modulation of innate immunity related genes resulting in prophylactic antimicrobial and antiviral properties
IF: 6.100 -
Anti-Tumor Immunity to Patient-Derived Breast Cancer Cells by Vaccination with Interferon-Alpha-Conditioned Dendritic Cells (IFN-DC)
IF: 5.200 -
Hypomethylation‐associated ELF3 helps nasopharyngeal carcinoma to escape immune surveillance via MUC16-mediated glycolytic metabolic reprogramming
IF: 5.000Journal: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY (2024)Reactivity:Human -
Relation of T Cell Profile with Vitamin D Receptor and Vitamin D-Binding Protein Gene Polymorphisms in Atopy
IF: 4.900Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity:HumanSample Type:Serum -
Cuproptosis in microsatellite stable colon cancer cells affects the cytotoxicity of CD8+T through the WNT signaling pathway
IF: 4.700 -
Interferon-γ in the tumor microenvironment promotes the expression of B7H4 in colorectal cancer cells, thereby inhibiting cytotoxic T cells
IF: 4.600Journal: Scientific Reports (2024)Sample Type:Peripheral blood mononuclear cells (PBMCs),human umbilical vein endothelial cells (HUVECs)