HIS(Histamine) ELISA Kit
中文名称:组胺(HIS)酶联免疫吸附测定试剂盒

Price:
- 反应性: Universal
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用HIS抗原包被于酶标板上,实验时样品或标准品中的HIS与包被的HIS竞争生物素标记的抗HIS单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,HIS浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中HIS的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 2 h 30 min |
反应性 | Universal |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的HIS,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Nasal solitary chemosensory cells govern daily rhythm in mouse model of allergic rhinitis
IF: 11.400Journal: JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY (2024)Reactivity:MouseSample Type:serum,nasal mucosa tissue -
Allergenicity assessment of β-lactoglobulin ferulic acid–glucose conjugates
IF: 8.500 -
Effect of varying dietary fatty acid patterns on early-life food allergy in rats
IF: 7.400 -
Reducing the Allergenicity of β-Lactoglobulin by Covalent Modification with Different Contents of Epigallocatechin Gallate (EGCG): In Vitro and In Vivo Studies
IF: 5.700Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity:MouseSample Type:serum -
Role of LECT2 in exacerbating atopic dermatitis: insight from in vivo and in vitro models via NF-κB signaling pathway
IF: 5.700Journal: Frontiers in Immunology (2024)Reactivity:MouseSample Type:serum -
Histamine promotes mouse decidualization through stimulating epithelial amphiregulin release
IF: 5.500 -
Assessing the impact of insect protein sources on intestinal health and disease: insights from human ex vivo and rat in vivo models
IF: 5.100 -
Cang-ai volatile oil alleviates nasal inflammation via Th1/Th2 cell imbalance regulation in a rat model of ovalbumin-induced allergic rhinitis
IF: 4.400Journal: Frontiers in Pharmacology (2024)Reactivity:RatSample Type:Serum -
Protective effect of rupatadine on testicular ischemia/reperfusion injury in rats: Modulation of IL-6/STAT3, Akt/ mTOR signaling pathways
IF: 3.300Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY (2024)Reactivity:RatSample Type:testicular tissue -
Desloratadine via its anti-inflammatory and antioxidative properties ameliorates TNBS-induced experimental colitis in rats
IF: 2.900Journal: IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2024)Reactivity:RatSample Type:colon tissue,plasma