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Human HMGB-1(High mobility group protein B1) ELISA Kit

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  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
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    产品货号:E-EL-H1554c

    中文名:人高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒

    别称:HMGB1, HMG1, HMG3, SBP-1, Amphoterin

    规格:
    • 96T
    • 48T
    数量:
    - +
    价格: ¥3000

    反应性:

    检测范围: 31.25~2000 pg/mL

    灵敏度: 18.75 pg/mL

    货期: 3个工作日+顺丰周期

    在线咨询 比较 加入购物车 说明书

    用途

    该试剂盒用于体外定量检测Human 血清,血浆或其他生物体液中天然及部分重组HMGB-1浓度

    检测原理

    本试剂盒采用双抗体夹心ELISA法。用抗人HMGB-1抗体包被于酶标板上,实验时样品或标准品中的人HMGB-1会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗人HMGB-1抗体和辣根过氧化物酶标记的亲和素。抗人HMGB-1抗体与结合在包被抗体上的人HMGB-1结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,HMGB-1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中HMGB-1的浓度。

    反应类型 夹心法
    规格 96T
    反应时间 3.5h
    反应性 Human
    检测方法 Colormetric
    检测范围 31.25—2000 pg/mL
    灵敏度 18.75 pg/mL
    样本体积 100μL
    样本类型 血清,血浆或其他生物体液

    特异性

    可检测样本中的人HMGB-1,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average Corrected
    2000 2.443
    2.447
    2.445 2.374
    1000 1.718
    1.764
    1.741 1.67
    500 0.988
    0.984
    0.986 0.915
    250 0.466
    0.498
    0.482 0.411
    125 0.265
    0.247
    0.256 0.185
    62.5 0.187
    0.161
    0.174 0.103
    31.25 0.118
    0.13
    0.124 0.053
    0 0.06900000000000001
    0.073
    0.07099999999999999 --

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

    Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    99.32 202.75 693.26 108.96 208.03 746.59
    Standard
    deviation
    5.84 8.62 35.56 5.86 9.76 26.06
    C V (%) 5.88 4.25 5.13 5.38 4.69 3.49

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=5) 94-108 100
    EDTA plasma (n=5) 94-107 100
    Cell culture media (n=5) 93-105 99

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 98-111 92-105 85-100
    Average (%) 105 98 92
    1:4 Range (%) 90-103 84-100 88-102
    Average (%) 98 91 94
    1:8 Range (%) 87-99 83-93 85-96
    Average (%) 94 88 90
    1:16 Range (%) 88-103 85-96 86-99
    Average (%) 94 90 91

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2 支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 2-8℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 2-8℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 2-8℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1. 酶标仪(450nm波长滤光片)
    • 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3. 37℃恒温箱,双蒸水或去离子水
    • 4. 吸水纸

    操作步骤

    1. 对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟

    2. 弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟

    3. 弃掉板内液体,洗板3次

    4. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次

    5. 每孔加入90μL底物溶液,37℃孵育15分钟左右

    6. 每孔加入50μL终止液

    7. 立即在450nm波长下读数,处理数据

    客户发表文献

    1. Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma.
      IF: 8.805999999
      Journal: Biomaterials(2018)
      DOI: 10.1016/j.biomaterials.2018.07.035
      PMID: 30071380
      引用产品: E-EL-H1554c 
      样本类型: Cell culture medium
      物种: Human
    2. Harnessing The Cross-Talk Between Tumor Cells And Tumor-Associated Macrophages With A Nano-Drug For Modulation Of Glioblastoma Immune Microenvironment.
      IF: 7.786
      Journal: Journal of Controlled Release(2017)
      DOI: 10.1016/j.jconrel.2017.10.024
      PMID: 29051064
      引用产品: E-EL-H1554c 
      样本类型: Cell culture supernatant
      物种: Human
    3. Doxorubicin conjugated with nanodiamonds and in free form commit glioblastoma cells to heterodromous fates
      IF: 5.005
      Journal: Nanomedicine(2019)
      DOI: 10.2217/nnm-2018-0330
      PMID: 30676239
      引用产品: E-EL-H1554c 
      样本类型: Cell culture medium
      物种: Human
    4. Profiling of Canonical and Non-Traditional Cytokine Levels in Interferon-β-Treated Relapsing–Remitting-Multiple Sclerosis Patients.
      IF: 5.511
      Journal: Frontiers in Immunology(2018)
      DOI: 10.3389/fimmu.2018.01240
      PMID: 29915590
      引用产品: E-EL-H1554c 
      样本类型: Serum
      物种: Human
    5. Young Alcohol Binge Drinkers Have Elevated Blood Endotoxin, Peripheral Inflammation And Low Cortisol Levels: Neuropsychological Correlations In Women.
      IF: 4.603
      Journal: Addiction Biology(2017)
      DOI: 10.1111/adb.12543
      PMID: 28840951
      引用产品: E-EL-H1554c 
      样本类型: Plasma
      物种: Human
    6. Synthetic Anti-Endotoxin Peptides Inhibit Cytoplasmic LPS-Mediated Responses.
      IF: 4.581
      Journal: Biochemical Pharmacology(2017)
      DOI: 10.1016/j.bcp.2017.05.015
      PMID: 28539262
      引用产品: E-EL-H1554c 
      样本类型: Cell culture supernatant
      物种: Human
    7. The association between plasma HMGB1 and sRAGE and clinical outcome in intracerebral hemorrhage
      IF: 3.125
      Journal: Journal of Neuroimmunology(2020)
      DOI: 10.1016/j.jneuroim.2020.577266
      PMID: 99999999
      引用产品: E-EL-H1554c 
      样本类型: Plasma
      物种: Human
    8. Role of Toll-like receptor 4 in the regulation of the cell death pathway and neuroinflammation
      IF: 3.44
      Journal: Brain Research Bulletin(2019)
      DOI: 10.1016/j.brainresbull.2019.03.015
      PMID: 30940475
      引用产品: E-EL-H1554c 
      样本类型: Cell culture medium
      物种: Rat
    9. Short-Term Postoperative Cognitive Dysfunction And Inflammatory Response In Patients Undergoing Cytoreductive Surgery And Hyperthermic Intraperitoneal Chemotherapy: A Pilot Study.
      IF: 3.232
      Journal: Mediators Of Inflammation(2017)
      DOI: 10.1155/2017/3605350
      PMID: 29249869
      引用产品: E-EL-H0149c E-EL-H0109c E-EL-H1554c E-EL-H2183c E-EL-H1297c 
      样本类型: Serum
      物种: Human
    10. Ethanol Directly Induced HMGB1 Release Through NOX2/NLRP1 Inflammasome In Neuronal Cells.
      IF: 3.582
      Journal: Toxicology(2015)
      DOI: 10.1016/j.tox.2015.06.006
      PMID: 26079697
      引用产品: E-EL-H1554c E-EL-R0505c 
      样本类型: Cell culture supernatant
      物种: Human,Rat
    11. HMGB1 and SEPP1 as predictors of hepatocellular carcinoma in patients with viral C hepatitis: Effect of DAAs
      IF: 2.573
      Journal: Clinical biochemistry(2019)
      DOI: 10.1016/j.clinbiochem.2019.05.017
      PMID: 31158358
      引用产品: E-EL-H1554c E-EL-H2177c 
      样本类型: Serum
      物种: Human
    12. Glycyrrhizin suppresses epithelial-mesenchymal transition by inhibiting high-mobility group box1 via the TGF- β 1/Smad2/3 pathway in lung epithelial cells
      IF: 2.379
      Journal: PeerJ(2020)
      DOI: 10.7717/peerj.8514
      PMID: 32117622
      引用产品: E-EL-H1554c 
      样本类型: Cell culture supernatant
      物种: Human
    13. Recombined humanized endostatin-induced suppression of HMGB1 expression inhibits proliferation of NSCLC cancer cells
      IF: 2.569
      Journal: Thoracic cancer(2019)
      DOI: 10.1111/1759-7714.12905
      PMID: 30485686
      引用产品: E-EL-H1554c 
      样本类型: Cell culture supernatant
      物种: Human
    14. Relationships Of BRAF Mutation And HMGB1To Papillary Thyroid Carcinoma.
      IF: 2.466
      Journal: Biochemical And Biophysical Research Communications(2017)
      DOI: 10.1016/j.bbrc.2017.03.117
      PMID: 28342873
      引用产品: E-EL-H1554c 
      样本类型: Serum
      物种: Human
    15. Hmgb1 Enhances Drug Resistance And Promotes In Vivo Tumor Growth Of Lung Cancer Cells.
      IF: 2.236
      Journal: DNA and Cell Biology(2016)
      DOI: 10.1089/dna.2016.3360
      PMID: 27383136
      引用产品: E-EL-H1554c 
      样本类型: Serum,Cell culture supernatant
      物种: Human
    16. Association of High Mobility Group Box Protein B1 Gene Polymorphisms with Pneumonia Susceptibility and Severity
      IF: 1.181
      Journal: Genetic testing and molecular biomarkers(2018)
      DOI: 10.1089/gtmb.2018.0174
      PMID: 30562142
      引用产品: E-EL-H0103c E-EL-H0109c E-EL-H0192c E-EL-H1554c 
      样本类型: Plasma
      物种: Human
    17. Triptolide suppresses growth of breast cancer by targeting HMGB1 in vitro and in vivo
      IF: 1.694
      Journal: Biological and Pharmaceutical Bulletin(2019)
      DOI: 10.1248/bpb.b18-00818
      PMID: 30956264
      引用产品: E-EL-H1554c 
      样本类型: Cell culture supernatant
      物种: Human
    18. Blood Purification Treatment Initiated At The Time Of Sepsis Diagnosis Effectively Attenuates Serum HMGB1 Upregulation And Improves Patient Prognosis.
      IF: 1.261
      Journal: Experimental and Therapeutic Medicine(2017)
      DOI: 10.3892/etm.2017.4854
      PMID: 28912856
      引用产品: E-EL-H1554c 
      样本类型: Serum,Urine
      物种: Human
    19. Necrosis Of Osteosarcoma Cells Induces The Production And Release Of HighMobility Group Box 1 Protein.
      IF: 1.261
      Journal: Experimental and Therapeutic Medicine(2018)
      DOI: 10.3892/etm.2017.5415
      PMID: 29387198
      引用产品: E-EL-H1554c 
      样本类型: Cell culture supernatant
      物种: Human
    20. Cigarette smoke-induced HMGB1 translocation and release contribute to migration and NF-κB activation through inducing autophagy in lung macrophages
      Journal: Journal of Cellular and Molecular Medicine(2020)
      DOI: 10.1111/jcmm.14789
      PMID: 31769590
      引用产品: E-EL-H1554c E-EL-M0676c 
      样本类型: Serum,Cell culture supernatant
      物种: Human,Mouse
    21. High-Mobility Group Protein Box 1 is Upregulated in Children with Henoch-Schonlein Purpura.
      IF: 0.533
      Journal: Pediatric Allergy(2018)
      DOI: 10.1089/ped.2018.0872
      引用产品: E-EL-H0109c E-EL-H1554c E-EL-H0102c 
      样本类型: Serum
      物种: Human
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