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Human IFN-γ(Interferon Gamma) ELISA Kit

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  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
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  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
    产品货号:E-EL-H0108c

    中文名:人γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒

    别称:IFNG, IFG, IFI, Type II Interferon

    规格:
    • 96T
    • 48T
    数量:
    - +
    价格: ¥2400

    反应性:

    检测范围: 15.63~1000 pg/mL

    灵敏度: 9.38 pg/mL

    货期: 3个工作日+顺丰周期

    加入购物车 比较 咨询 说明书

    用途

    该试剂盒用于体外定量检测Human 血清,血浆或其他生物体液中天然及部分重组IFN-γ浓度

    检测原理

    本试剂盒采用双抗体夹心ELISA法。用抗人IFN-γ抗体包被于酶标板上,实验时样品或标准品中的人IFN-γ会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗人IFN-γ抗体和辣根过氧化物酶标记的亲和素。抗人IFN-γ抗体与结合在包被抗体上的人IFN-γ结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IFN-γ浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IFN-γ的浓度。

    反应类型 夹心法
    规格 96T
    反应时间 3.5h
    反应性 Human
    检测方法 Colormetric
    检测范围 15.63—1000 pg/mL
    灵敏度 9.38 pg/mL
    样本体积 100μL
    样本类型 血清,血浆或其他生物体液

    特异性

    可检测样本中的人IFN-γ,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average Corrected
    1000 2.475
    2.517
    2.496 2.438
    500 1.552
    1.602
    1.577 1.519
    250 0.963
    0.953
    0.958 0.9
    125 0.463
    0.487
    0.475 0.417
    62.5 0.251
    0.241
    0.246 0.188
    31.25 0.171
    0.145
    0.158 0.1
    15.63 0.107
    0.113
    0.11 0.052
    0 0.048
    0.068
    0.058 --

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

    Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    51.90 98.00 447.20 55.80 100.10 476.70
    Standard
    deviation
    3.20 4.80 16.10 2.80 5.40 24.30
    C V (%) 6.17 4.90 3.60 5.02 5.39 5.10

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=5) 96-107 101
    EDTA plasma (n=5) 89-104 96
    Cell culture media (n=5) 86-97 91

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 99-114 92-105 89-105
    Average (%) 105 98 96
    1:4 Range (%) 98-110 86-99 91-105
    Average (%) 103 92 97
    1:8 Range (%) 96-110 82-98 98-114
    Average (%) 102 89 104
    1:16 Range (%) 97-111 85-99 95-109
    Average (%) 105 90 102

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2 支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 2-8℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 2-8℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 2-8℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1. 酶标仪(450nm波长滤光片)
    • 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3. 37℃恒温箱,双蒸水或去离子水
    • 4. 吸水纸

    操作步骤

    1. 对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟

    2. 弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟

    3. 弃掉板内液体,洗板3次

    4. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次

    5. 每孔加入90μL底物溶液,37℃孵育15分钟左右

    6. 每孔加入50μL终止液

    7. 立即在450nm波长下读数,处理数据

    客户发表文献

    1. Decreased Expression Of Programmed Death 1 On Peripheral Blood Lymphocytes Disrupts Immune Homeostasis In Peripartum Cardiomyopathy.
      IF: 6.189
      Journal: International Journal of Cardiology(2016)
      DOI: 10.1016/j.ijcard.2016.08.232
      PMID: 27580218
      引用产品: E-EL-H0108c E-EL-H0101c 
      样本类型: Serum
      物种: Human
    2. Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils
      IF: 5.546
      Journal: Journal of Cellular Physiology(2020)
      DOI: 10.1002/jcp.29713
      PMID: 32385929
      引用产品: E-EL-H0088c E-EL-H0099c E-EL-H0101c E-EL-H0102c E-EL-H0108c E-EL-H0109c E-EL-H0110c E-EL-H6008 
      样本类型: Cell culture supernatant
      物种: Human
    3. S-adenosyl-L-methionine (SAMe) halts the autoimmune response in patients with primary biliary cholangitis (PBC) via antioxidant and S-glutathionylation processes in cholangiocytes
      IF: 4.352
      Journal: Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease(2020)
      DOI: 10.1016/j.bbadis.2020.165895
      PMID: 32681864
      引用产品: E-EL-H0103c E-EL-H0108c E-EL-H0110c 
      样本类型: Serum
      物种: Human
    4. Glutamine Deprivation Induces PD-L1 Expression via Activation of EGFR/ERK/c-Jun Signaling in Renal Cancer
      IF: 4.63
      Journal: Molecular Cancer Research(2020)
      DOI: 10.1158/1541-7786.MCR-19-0517
      PMID: 31672701
      引用产品: E-EL-H0108c 
      样本类型: Cell culture supernatant
      物种: Human
    5. Relationship between immune parameters during a severe exacerbation in allergic asthmatic children and asthma outcomes in the following year
      IF: 4.217
      Journal: Clinical & Experimental Allergy(2020)
      DOI: 10.1111/cea.13570
      PMID: 31955476
      引用产品: E-EL-H0085c E-EL-H0102c E-EL-H0107c E-EL-H0108c E-EL-H0149c E-EL-H5509c E-EL-H5812c 
      样本类型: Plasma
      物种: Human
    6. miR-A-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis
      IF: 3.967
      Journal: Apoptosis(2018)
      DOI: 10.1007/s10495-018-1474-7
      PMID: 30167920
      引用产品: E-EL-H0108c E-EL-H0109c E-EL-R0009c E-EL-R0019c 
      样本类型: Serum
      物种: Human,Rat
    7. Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome
      IF: 3.923
      Journal: Journal of cellular physiology(2019)
      DOI: 10.1002/jcp.27501
      PMID: 30580448
      引用产品: E-EL-H0108c E-EL-R0009c 
      样本类型: Serum
      物种: Human,Rat
    8. Association Between IFN-γ Gene Polymorphisms And IgA Nephropathy In A Chinese Han Population.
      IF: 3.104
      Journal: Kidney & Blood Pressure Research(2017)
      DOI: 10.1159/000473889
      PMID: 28391282
      引用产品: E-EL-H0108c 
      样本类型: Serum
      物种: Human
    9. Platelet Lysate Supports The In Vitro Expansion Of Human Periodontal Ligament Stem Cells For Cytotherapeutic Use.
      IF: 3.989
      Journal: Journal of Tissue Engineering And Regenerative Medicine(2017)
      DOI: 10.1002/term.2124
      PMID: 26833905
      引用产品: E-EL-H0108c 
      样本类型: Cell culture supernatant
      物种: Human
    10. Platelet Lysate Supports The In Vitro Expansion Of Human Periodontal Ligament Stem Cells For Cytotherapeutic Use.
      IF: 3.989
      Journal: Journal of Tissue Engineering And Regenerative Medicine(2016)
      DOI: 10.1002/term.2124
      PMID: 26833905
      引用产品: E-EL-H0108c 
      样本类型: Cell culture supernatant
      物种: Human
    11. Clonorchis Sinensis Co-Infection Could Affect The Disease State And Treatment Response Of Hbv Patients.
      IF: 3.834
      Journal: Plos Neglected Tropical Diseases(2016)
      DOI: 10.1371/journal.pntd.0004806
      PMID: 27348302
      引用产品: E-EL-H0099c E-EL-H0101c E-EL-H0102c E-EL-H0103c E-EL-H0108c 
      样本类型: Serum,Cell culture supernatant
      物种: Human
    12. The role of the kynurenine pathway and quinolinic acid in adolescent major depressive disorder
      IF: 2.444
      Journal: International Journal of Clinical Practice(2020)
      DOI: 10.1111/ijcp.13739
      PMID: 32997876
      引用产品: E-EL-H0108c 
      样本类型: Serum
      物种: Human
    13. Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China
      IF: 2.082
      Journal: Journal of Zhejiang University. Science. B(2020)
      DOI: 10.1631/jzus.B2000174
      PMID: 32425003
      引用产品: E-EL-H0043c E-EL-H0099c E-EL-H0101c E-EL-H0102c E-EL-H0103c E-EL-H0108c E-EL-H0109c E-EL-H0866c 
      样本类型: Serum,Plasma
      物种: Human
    14. Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line
      IF: 2.639
      Journal: Biological Trace Element Research(2019)
      DOI: 10.1007/s12011-019-01831-2
      PMID: 31473895
      引用产品: E-EL-H0102c E-EL-H0108c E-EL-H0109c E-EL-H0149c E-EL-H0595c 
      样本类型: Cell lysate
      物种: Human
    15. Assessment of chemokine and cytokine signatures in patients with dengue infection: A hospital-based study in Kolkata, India
      IF: 2.509
      Journal: Acta tropica(2019)
      DOI: 10.1016/j.actatropica.2018.10.017
      PMID: 30395811
      引用产品: E-EL-H0023c E-EL-H0048c E-EL-H0102c E-EL-H0103c E-EL-H0108c E-EL-H0150c 
      样本类型: Serum
      物种: Human
    16. The elevated glutaminolysis of bladder cancer and T cells in a simulated tumor microenvironment contributes to the up-regulation of PD-l1 expression by interferon-γ
      IF: 2.66
      Journal: OncoTargets and Therapy(2018)
      DOI: 10.2147/OTT.S180505
      PMID: 30425515
      引用产品: E-EL-H0108c 
      样本类型: Cell culture supernatant
      物种: Human
    17. C Reactive Protein And Enzymatically Modified LDL Cooperatively Promote Dendritic Cell-Mediated T Cell Activation.
      IF: 2.359
      Journal: Cardiovascular Pathology(2017)
      DOI: 10.1016/j.carpath.2017.03.009
      PMID: 28433861
      引用产品: E-EL-H0108c E-EL-H0109c E-EL-H0150c E-EL-H0102c 
      样本类型: Cell culture supernatant
      物种: Human
    18. Expression Of Programmed Cell Death-1 And Its Ligand B7 Homolog 1 In Peripheral Blood Lymphocytes From Patients With Peripartum Cardiomyopathy.
      IF: 2.757
      Journal: Clinical Cardiology(2016)
      DOI: 10.1002/clc.22661
      PMID: 28026044
      引用产品: E-EL-H0108c E-EL-H0101c 
      样本类型: Serum
      物种: Human
    19. Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells
      IF: 1.287
      Journal: Journal of International Medical Research(2020)
      DOI: 10.1177/0300060520930853
      PMID: 32663414
      引用产品: E-EL-H0099c E-EL-H0108c E-EL-H2306c 
      样本类型: Cell culture supernatant
      物种: Human
    20. Programmed cell death protein-1/programmed death-ligand 1 blockade enhances the antitumor efficacy of adoptive cell therapy against non-small cell lung cancer
      IF: 1.804
      Journal: Journal of thoracic disease(2018)
      DOI: 10.21037/jtd.2018.10.111
      PMID: 30746216
      引用产品: E-EL-H0108c 
      样本类型: Cell culture supernatant
      物种: Human
    21. Leukocyte Depletion Does Not Affect The In Vitro Healing Ability Of Platelet Rich Plasma.
      IF: 1.785
      Journal: Experimental and Therapeutic Medicine(2018)
      DOI: 10.3892/etm.2018.5887
      PMID: 29556269
      引用产品: E-EL-H0108c E-EL-H1577c E-EL-H1575c E-EL-H1670c E-EL-0162c E-EL-H0109c E-EL-H0092c 
      物种: Human
    22. The Shortening Telomere Length Of T Lymphocytes Maybe Associated With HyperFunction In Servere Aplastic Anemia.
      IF: 1.692
      Journal: Molecular Medicine Reports(2018)
      DOI: 10.3892/mmr.2017.8014
      PMID: 29115638
      引用产品: E-EL-H0108c E-EL-H0109c 
      物种: Human
    23. Inflammatory Characteristics In Different Types Of Nonallergic Rhinitis.
      IF: 1.706
      Journal: International Journal of Clinical and Experimental Pathology(2017)
      引用产品: E-EL-H0108c E-EL-H0101c E-EL-H5812c E-EL-H0103c 
      样本类型: Cell culture supernatant
      物种: Human
    24. Hematological Changes, Serum Interferon Gamma and Interleukin-4 Alterations in Normal Pregnancy and Preeclampsia
      Journal: International Electronic Journal of Medicine(2020)
      DOI: 10.34172/iejm.2020.08
      PMID: 99999999
      引用产品: E-EL-H0101c E-EL-H0108c E-EL-H0400c 
      样本类型: Serum
      物种: Human
    25. High Levels of Tumor Necrosis Factor-Alpha and Interferon-Gamma in Patients with Gingival Recession
      Journal: Medico Legal Update(2020)
      DOI: 10.37506/v20/i1/2020/mlu/194424
      PMID: 99999999
      引用产品: E-EL-H0108c E-EL-H0109c 
      样本类型: Serum
      物种: Human
    26. Correlation between the percentage of memory T cells and IFN-γ level in patients with systemic lupus erythematous
      Journal: Journal of Physics: Conference Series(2019)
      DOI: 10.1088/1742-6596/1246/1/012015/meta
      PMID: 99999999
      引用产品: E-EL-H0108c 
      样本类型: Serum
      物种: Human
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