Human IFNα(Interferon Alpha ) ELISA Kit
中文名称:人干扰素α(IFNα)酶联免疫吸附测定试剂盒
别称:
IFL、IFNA、IFN、IFNalpha
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.69 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFNα抗体包被于酶标板上,实验时样品(或标准品)中的人IFNα会与包被抗体结合。后依次加入生物素化的抗人IFNα抗体和辣根过氧化物酶标记的亲和素,抗人IFNα抗体与结合在包被抗体上的人IFNα结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IFNα浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IFNα的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人IFNα,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Differential immune responses in pregnant patients recovered from COVID-19
IF: 18.187 -
A SCID mouse-human lung xenograft model of SARS-CoV-2 infection
IF: 11.556 -
2-O-Methylhonokiol Suppresses HCV Replication via TRAF6-Mediated NF-kB Activation
IF: 5.924 -
Potential Involvement of Platelet-Derived Microparticles and Microparticles Forming Immune Complexes during Monocyte Activation in Patients with Systemic Lupus Erythematosus
IF: 5.511Journal:Frontiers in Immunology -
miR-31-5p Regulates Type I Interferon by Targeting SLC15A4 in Plasmacytoid Dendritic Cells of Systemic Lupus Erythematosus
IF: 4.631Journal:Journal of Inflammation Research -
Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?
IF: 3.657 -
Anti-inflammatory effects of nobiletin on TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways in prostate cancer cells
IF: 2.352 -
Functional characterisation of Holothuria leucospilota Fas-associated death domain in the innate immune-related signalling pathways:
IF: 2.173Journal:Innate Immunity
Q1: Human IFNα(Interferon Alpha ) ELISA Kit,Which subtype of IFNα is detected? If the test is total, is the criterion a mixture?
This indicator measures all subtypes of IFNα, that is, total IFNα. The standard is not a mixture of all subtypes, but human IFN-alpha 2a (recombinant protein, expression system: HEK293 cells). However, since the homology of the 15 IFN-α subtypes in humans is as high as 80%, the antibodies used in our kit can recognize all subtypes, and this kit has been verified for different subtypes of IFN-α, which can be detected.