• 本公司产品仅供体外研究使用,不用于临床诊断

Human IL-10(Interleukin 10) ELISA Kit

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  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
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  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
    产品货号:E-EL-H0103c

    中文名:人白介素10(IL-10)酶联免疫吸附测定试剂盒

    别称:IL10, IL10A, CSIF, TGIF

    规格:
    • 96T
    • 48T
    数量:
    - +
    价格: ¥2400

    反应性:

    检测范围: 7.81~500 pg/mL

    灵敏度: 4.69 pg/mL

    货期: 3个工作日+顺丰周期

    加入购物车 比较 咨询 说明书

    用途

    该试剂盒用于体外定量检测Human 血清,血浆或其他生物体液中天然及部分重组IL-10浓度

    检测原理

    本试剂盒采用双抗体夹心ELISA法。用抗人IL-10抗体包被于酶标板上,实验时样品或标准品中的人IL-10会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗人IL-10抗体和辣根过氧化物酶标记的亲和素。抗人IL-10抗体与结合在包被抗体上的人IL-10结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IL-10浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-10的浓度。

    反应类型 夹心法
    规格 96T
    反应时间 3.5h
    反应性 Human
    检测方法 Colormetric
    检测范围 7.81—500 pg/mL
    灵敏度 4.69 pg/mL
    样本体积 100μL
    样本类型 血清,血浆或其他生物体液

    特异性

    可检测样本中的人IL-10,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average Corrected
    500 2.529
    2.565
    2.547 2.488
    250 1.595
    1.649
    1.622 1.563
    125 0.997
    0.975
    0.986 0.927
    62.5 0.508
    0.546
    0.527 0.468
    31.25 0.273
    0.251
    0.262 0.203
    15.63 0.163
    0.163
    0.163 0.104
    7.81 0.109
    0.115
    0.112 0.053
    0 0.052
    0.066
    0.059 --

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

    Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    26.20 42.70 233.00 26.00 44.20 228.10
    Standard
    deviation
    1.80 2.60 7.70 1.70 2.00 7.10
    C V (%) 6.87 6.09 3.30 6.54 4.52 3.11

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=5) 88-101 96
    EDTA plasma (n=5) 83-95 90
    Cell culture media (n=5) 92-105 99

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 94-109 87-101 85-96
    Average (%) 102 92 91
    1:4 Range (%) 95-109 85-97 95-105
    Average (%) 100 91 100
    1:8 Range (%) 95-110 84-98 95-108
    Average (%) 102 91 103
    1:16 Range (%) 98-111 81-95 93-108
    Average (%) 104 88 101

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2 支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 2-8℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 2-8℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 2-8℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1. 酶标仪(450nm波长滤光片)
    • 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3. 37℃恒温箱,双蒸水或去离子水
    • 4. 吸水纸

    操作步骤

    1. 对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟

    2. 弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟

    3. 弃掉板内液体,洗板3次

    4. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次

    5. 每孔加入90μL底物溶液,37℃孵育15分钟左右

    6. 每孔加入50μL终止液

    7. 立即在450nm波长下读数,处理数据

    客户发表文献

    1. S-adenosyl-L-methionine (SAMe) halts the autoimmune response in patients with primary biliary cholangitis (PBC) via antioxidant and S-glutathionylation processes in cholangiocytes
      IF: 4.352
      Journal: Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease(2020)
      DOI: 10.1016/j.bbadis.2020.165895
      PMID: 32681864
      引用产品: E-EL-H0103c E-EL-H0108c E-EL-H0110c 
      样本类型: Serum
      物种: Human
    2. Treating periodontal disease in patients with myocardial infarction: A randomized clinical trial
      IF: 4.329
      Journal: European Journal of Internal Medicine(2020)
      DOI: 10.1016/j.ejim.2019.08.012
      PMID: 31810741
      引用产品: E-EL-H0102c E-EL-H0103c E-EL-H0149c 
      样本类型: Serum
      物种: Human
    3. Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals
      IF: 4.234
      Journal: Diabetes research and clinical practice(2019)
      DOI: 10.1016/j.diabres.2019.05.023
      PMID: 31150725
      引用产品: E-EL-H0086c E-EL-H0102c E-EL-H0103c E-EL-H0109c E-EL-H0456c E-EL-H1213c E-EL-H1763c E-EL-H5811c E-EL-H6017 
      样本类型: Serum
      物种: Human
    4. Mesenchymal Stem Cells (MSCs) Coculture Protects [Ca2+] i Orchestrated Oxidant Mediated Damage in Differentiated Neurons In Vitro
      IF: 4.829
      Journal: Cells(2018)
      DOI: 10.3390/cells7120250
      PMID: 30563298
      引用产品: E-EL-H0102c E-EL-H0103c 
      样本类型: Cell culture supernatant
      物种: Human
    5. Multifunctional Dextran Sulfate-Coated Reconstituted High Density Lipoproteins Target Macrophages And Promote Beneficial Antiatherosclerotic Mechanisms.
      IF: 4.818
      Journal: Bioconjugate Chemistry(2016)
      DOI: 10.1021/acs.bioconjchem.6b00600
      PMID: 28004910
      引用产品: E-EL-H0109c E-EL-H0102c E-EL-H0103c 
      样本类型: Cell culture supernatant
      物种: Human
    6. IL-16 regulates macrophage polarization as a target gene of miR-145-3p
      IF: 3.188
      Journal: Molecular immunology(2019)
      DOI: 10.1016/j.molimm.2018.12.027
      PMID: 30634164
      引用产品: E-EL-H0102c E-EL-H0103c E-EL-H0109c E-EL-H0150c E-EL-H0235c 
      样本类型: Cell lysate
      物种: Human
    7. Pkcζ In Prostate Cancer Cells Represses The Recruitment And M2 Polarization Of Macrophages In The Prostate Cancer Microenvironment.
      IF: 3.65
      Journal: Tumor Biology(2017)
      DOI: 10.1177/1010428317701442
      PMID: 28631559
      引用产品: E-AB-32609 E-EL-H0101c E-EL-H0103c 
      样本类型: Cell culture supernatant,Cell lysate
      物种: Human
    8. Clonorchis Sinensis Co-Infection Could Affect The Disease State And Treatment Response Of Hbv Patients.
      IF: 3.834
      Journal: Plos Neglected Tropical Diseases(2016)
      DOI: 10.1371/journal.pntd.0004806
      PMID: 27348302
      引用产品: E-EL-H0099c E-EL-H0101c E-EL-H0102c E-EL-H0103c E-EL-H0108c 
      样本类型: Serum,Cell culture supernatant
      物种: Human
    9. Inhibitory Effect Of miR-125b On Hepatitis C Virus Core Protein-Induced TLR2/MyD88 Signaling In THP-1 Macrophages.
      IF: 3.365
      Journal: World Journal of Gastroenterology(2016)
      DOI: 10.3748/wjg.v22.i17.4354
      PMID: 27158204
      引用产品: E-EL-H0109c E-EL-H0102c E-EL-H0103c 
      样本类型: Cell culture supernatant
      物种: Human
    10. Intermittent hypoxia induces tumor immune escape in murine S180 solid tumors via the upregulation of TGF-β 1 in mice
      IF: 2.162
      Journal: Sleep and Breathing(2020)
      DOI: 10.1007/s11325-020-02166-2
      PMID: 32840731
      引用产品: E-EL-H0102c E-EL-H0103c E-EL-H0109c E-EL-H5812c 
      样本类型: Tissue homogenate
      物种: Mouse
    11. Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China
      IF: 2.082
      Journal: Journal of Zhejiang University. Science. B(2020)
      DOI: 10.1631/jzus.B2000174
      PMID: 32425003
      引用产品: E-EL-H0043c E-EL-H0099c E-EL-H0101c E-EL-H0102c E-EL-H0103c E-EL-H0108c E-EL-H0109c E-EL-H0866c 
      样本类型: Serum,Plasma
      物种: Human
    12. A case of asthma with Beh?et's disease: successful treatment with omalizumab and its effects on recurrent aphthous lesions
      IF: 2.352
      Journal: Immunopharmacology and Immunotoxicology(2020)
      DOI: 10.1080/08923973.2020.1789656
      PMID: 32605504
      引用产品: E-EL-H0048c E-EL-H0102c E-EL-H0103c E-EL-H0107c E-EL-H0149c E-EL-H1648c E-EL-H2402c E-EL-H5812c 
      样本类型: Serum
      物种: Human
    13. Major Depression in Children with Transfusion-Dependent Thalassemia Is Strongly Associated with the Combined Effects of Blood Transfusion Rate, Iron Overload, and Increased pro-inflammatory Cytokines
      IF: 2.992
      Journal: Neurotoxicity research(2020)
      DOI: 10.1007/s12640-020-00193-1
      PMID: 32335809
      引用产品: E-EL-H0025c E-EL-H0103c E-EL-H0109c 
      样本类型: Serum
      物种: Human
    14. Isolation, characterization and analysis of pro-inflammatory potential of Klebsiella pneumoniae outer membrane vesicles
      IF: 2.914
      Journal: Microbial pathogenesis(2019)
      DOI: 10.1016/j.micpath.2019.103719
      PMID: 31493501
      引用产品: E-EL-H0048c E-EL-H0102c E-EL-H0103c E-EL-H0109c 
      样本类型: Cell culture supernatant
      物种: Human
    15. Assessment of chemokine and cytokine signatures in patients with dengue infection: A hospital-based study in Kolkata, India
      IF: 2.509
      Journal: Acta tropica(2019)
      DOI: 10.1016/j.actatropica.2018.10.017
      PMID: 30395811
      引用产品: E-EL-H0023c E-EL-H0048c E-EL-H0102c E-EL-H0103c E-EL-H0108c E-EL-H0150c 
      样本类型: Serum
      物种: Human
    16. In Vitro Immunomodulatory Effect of Food Supplement from Aloe vera
      IF: 2.064
      Journal: Evidence-Based Complementary and Alternative Medicine(2019)
      DOI: 10.1155/2019/5961742
      PMID: 30941196
      引用产品: E-EL-H0103c E-EL-H0109c E-EL-H0149c 
      样本类型: Cell culture supernatant
      物种: Human
    17. Decreased Monocyte Subsets And TLR4-Mediated Functions In Patients With Acute Severe Fever With Thrombocytopenia Syndrome (SFTS).
      IF: 2.532
      Journal: International Journal of Infectious Diseases(2015)
      DOI: 10.1016/j.ijid.2015.12.009
      PMID: 26701820
      引用产品: E-EL-H0109c E-EL-H0103c 
      样本类型: Cell culture supernatant
      物种: Human
    18. Systematically transplanted human gingiva-derived mesenchymal stem cells regulate lipid metabolism and inflammation in hyperlipidemic mice with periodontitis
      IF: 1.785
      Journal: Experimental and Therapeutic Medicine(2020)
      DOI: 10.3892/etm.2019.8256
      PMID: 31885706
      引用产品: E-EL-H0102c E-EL-H0103c E-EL-H0109c 
      样本类型: Serum
      物种: Human
    19. Association of High Mobility Group Box Protein B1 Gene Polymorphisms with Pneumonia Susceptibility and Severity
      IF: 1.181
      Journal: Genetic testing and molecular biomarkers(2018)
      DOI: 10.1089/gtmb.2018.0174
      PMID: 30562142
      引用产品: E-EL-H0103c E-EL-H0109c E-EL-H0192c E-EL-H1554c 
      样本类型: Plasma
      物种: Human
    20. Inflammatory Characteristics In Different Types Of Nonallergic Rhinitis.
      IF: 1.706
      Journal: International Journal of Clinical and Experimental Pathology(2017)
      引用产品: E-EL-H0108c E-EL-H0101c E-EL-H5812c E-EL-H0103c 
      样本类型: Cell culture supernatant
      物种: Human
    21. Induction of CD4 + CD25 + Regulatory T Cells from In Vitro Grown Human Mononuclear Cells by Sparteine Sulfate and Harpagoside
      Journal: Biology(2020)
      DOI: 10.3390/biology9080211
      PMID: 32781652
      引用产品: E-EL-H0103c E-EL-H0110c 
      样本类型: Cell culture supernatant
      物种: Human
    22. Single Nucleotide Polymorphisms in IL-10-1082 A\G Gene and Association with HCMV Infection in Abortion
      Journal: Indian Journal of Public Health Research & Development(2019)
      DOI: 10.5958/0976-5506.2019.02580.4
      PMID: 99999999
      引用产品: E-EL-H0103c 
      样本类型: Serum
      物种: Human
    23. THE REGULATION OF IMMUNE SYSTEM BY ANTI-INFLAMMATORY INTERLEUKINS IN HEART DISEASE PATIENTS INFECTED WITH TOXOPLASMOSIS
      Journal: International Journal of Research in Science and Technology(2020)
      DOI: 10.37648/ijrst.v10i02.003
      PMID: 99999999
      引用产品: E-EL-H0103c E-EL-H0150c 
      样本类型: Serum
      物种: Human
    24. Thymosin β10 promotes tumor-associated macrophages M2 conversion and proliferation via the PI3K/Akt pathway in lung adenocarcinoma
      Journal: Preprint(2020)
      DOI: 10.21203/rs.3.rs-34263/v1
      PMID: 99999999
      引用产品: E-EL-H0102c E-EL-H0103c E-EL-H0109c 
      样本类型: Cell culture supernatant
      物种: Human
    25. ASSOCIATION OF IL-101082G/A GENE POLYMORPHISM WITH ITS Serum LEVELS IN ASTHMA PATIENTS
      Journal: Biochemical and Cellular Archives (2017)
      DOI: 99999999
      PMID: 99999999
      引用产品: E-EL-H0103c 
      样本类型: Serum
      物种: Human
    26. CD4+ Foxp3+ T cells, interleukin-35 (IL-35) and IL-10 in systemic lupus erythematosus patients: Relation to disease activity
      Journal: The Egyptian Rheumatologist(2018)
      DOI: 10.1016/j.ejr.2018.08.001
      PMID: 99999999
      引用产品: E-EL-H0103c E-EL-H2443c 
      样本类型: Serum
      物种: Human
    27. Estimation of Levels of Interleukin-1 beta and Interleukin-10 in Sera of some Iraqi Patients with Chronic Rheumatoid Arthritis
      Journal: Iraqi Journal of Science(2018)
      DOI: 99999999
      PMID: 99999999
      引用产品: E-EL-H0103c E-EL-H0149c 
      样本类型: Serum
      物种: Human
    28. The Relationship between Cytokines and Toxoplasma Infection in Heart Disease Patients.
      Journal: Journal of THI-QAR SCIENCE(2017)
      引用产品: E-EL-H0103c E-EL-H0150c 
      样本类型: Serum
      物种: Human
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