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Human MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit

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    产品货号:E-EL-H6005

    中文名:人单核细胞趋化蛋白1(MCP-1)酶联免疫吸附测定试剂盒

    别称:CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF

    规格:
    • 96T
    • 48T
    • 96T*5
    数量:
    - +
    价格: ¥2400

    反应性:

    检测范围: 62.50~4000 pg/mL

    灵敏度: 37.50 pg/mL

    用途

    该试剂盒用于体外定量检测Human 血清,血浆或其他生物体液中MCP-1浓度

    检测原理

    本试剂盒采用双抗体夹心ELISA法。用抗人MCP-1抗体包被于酶标板上,实验时样品或标准品中的人MCP-1会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗人MCP-1抗体和辣根过氧化物酶标记的亲和素。抗人MCP-1抗体与结合在包被抗体上的人MCP-1结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,MCP-1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中MCP-1的浓度。

    反应类型 夹心法
    规格 96T
    反应时间 3.5h
    反应性 Human
    检测方法 Colormetric
    检测范围 62.50—4000 pg/mL
    灵敏度 37.50 pg/mL
    样本体积 100μL
    样本类型 血清,血浆或其他生物体液

    特异性

    可检测样本中的人MCP-1,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average Corrected
    4000 2.552
    2.507
    2.53 2.469
    2000 1.634
    1.645
    1.64 1.579
    1000 0.957
    0.946
    0.952 0.891
    500 0.487
    0.506
    0.497 0.436
    250 0.275
    0.268
    0.272 0.211
    125 0.19
    0.19
    0.19 0.129
    62.50 0.129
    0.113
    0.121 0.06
    0 0.057
    0.064
    0.061 --

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

    Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    198.91 488.33 1680.55 164.36 433.06 1730.78
    Standard
    deviation
    10.01 24.12 90.58 9.45 20.74 76.85
    C V (%) 5.03 4.94 5.39 5.75 4.79 4.44

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=8) 97-108 101
    EDTA plasma (n=8) 89-99 94
    Cell culture media (n=8) 87-95 92

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 98-110 89-102 91-104
    Average (%) 103 96 98
    1:4 Range (%) 87-98 90-101 94-100
    Average (%) 92 94 97
    1:8 Range (%) 90-100 94-103 101-111
    Average (%) 94 99 105
    1:16 Range (%) 89-99 84-96 89-101
    Average (%) 96 92 97

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2 支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 2-8℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 2-8℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 2-8℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1. 酶标仪(450nm波长滤光片)
    • 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3. 37℃恒温箱,双蒸水或去离子水
    • 4. 吸水纸

    操作步骤

    1. 对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟

    2. 弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟

    3. 弃掉板内液体,洗板3次

    4. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次

    5. 每孔加入90μL底物溶液,37℃孵育15分钟左右

    6. 每孔加入50μL终止液

    7. 立即在450nm波长下读数,处理数据

    客户发表文献

    1. KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1
      IF: 27.401
      Journal: Molecular Cancer(2021)
      DOI: 10.1186/s12943-021-01369-9
      PMID: 34006303
      样本类型: Cell culture supernatant
      物种: Human
    2. A SCID mouse-human lung xenograft model of SARS-CoV-2 infection
      IF: 11.556
      Journal: Theranostics(2021)
      DOI: 10.7150/thno.58321
      PMID: 33995679
      样本类型: Tissue homogenate
      物种: Human
    3. High mobility group box 1 derived mainly from platelet microparticles exacerbates microvascular obstruction in no reflow
      IF: 10.407
      Journal: THROMBOSIS RESEARCH(2022)
      DOI: 10.1016/j.thromres.2022.12.003
      样本类型: Human cardiac microvascular endothelial cells (HCMECs)
      物种: Human
    4. Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion
      IF: 8.4689
      Journal: Cell Death & Disease(2020)
      DOI: 10.1038/s41419-020-2665-z
      PMID: 32532953
      样本类型: Cell culture supernatant
      物种: Human
    5. Tumor-Contacted Neutrophils Promote Metastasis by a CD90-TIMP-1 Juxtacrine–Paracrine Loop
      IF: 8.911
      Journal: CLINICAL CANCER RESEARCH(2019)
      DOI: 10.1158/1078-0432.CCR-18-2544
      样本类型: tumor-infiltrating neutrophils (TINs),neutrophils
      物种: Human
    6. Antidyslipidemic, Antioxidant, and Anti-inflammatory Effects of Jelly Drink Containing Polyphenol-Rich Roselle Calyces Extract and Passion Fruit Juice with Pulp in Adults with Dyslipidemia: A Randomized, Double-Blind, Placebo-Controlled Trial.
      IF: 7.31
      Journal: Oxidative Medicine and Cellular Longevity(2022)
      DOI: 10.1155/2022/4631983
      样本类型: plasma
      物种: Human
    7. Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo
      IF: 6.208
      Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(2022)
      DOI: 10.3390/ijms232416134
      样本类型: THP-1 cells
      物种: Human
    8. The GBM Tumor Microenvironment as a Modulator of Therapy Response: ADAM8 Causes Tumor Infiltration of Tams through HB-EGF/EGFR-Mediated CCL2 Expression and Overcomes TMZ Chemosensitization in Glioblastoma
      IF: 6.575
      Journal: Cancers(2022)
      DOI: 10.3390/cancers14194910
      PMID: 36230833
      样本类型: U87MG cell,G1 cell
      物种: Human
    9. Safety and Effects of Lactobacillus paracasei TISTR 2593 Supplementation on Improving Cholesterol Metabolism and Atherosclerosis-Related Parameters in Subjects with Hypercholesterolemia: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial
      IF: 6.706
      Journal: Nutrients(2023)
      DOI: 10.3390/nu15030661
      样本类型: serum
      物种: Human
    10. Prunella vulgaris L. Attenuates Experimental Autoimmune Thyroiditis by Inhibiting HMGB1/TLR9 Signaling
      IF: 4.162
      Journal: Drug Design Development and Therapy(2021)
      DOI: 10.2147/DDDT.S325814
      PMID: 34764638
      样本类型: Nthy-ori 3–1 cells
      物种: Human
    11. Gynura procumbens (Lour.) Merr. extract attenuates monocyte adherence to endothelial cells through suppression of the NF-κB signaling pathway
      IF: 4.36
      Journal: JOURNAL OF ETHNOPHARMACOLOGY(2022)
      DOI: 10.1016/j.jep.2022.115391
      PMID: 35589022
      样本类型: HUVECs
      物种: Human
    12. Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis
      IF: 4.575
      Journal: INFLAMMATION RESEARCH(2021)
      DOI: 10.1007/s00011-021-01459-w
      PMID: 33852061
      样本类型: Serum,Bronchoalveolar Lavage Fluid
      物种: Mouse
    13. Young alcohol binge drinkers have elevated blood endotoxin, peripheral inflammation and low cortisol levels: neuropsychological correlations in women
      IF: 4.603
      Journal: ADDICTION BIOLOGY(2017)
      DOI: 10.1111/adb.12543
      样本类型: Plasma
      物种: Human
    14. Common variants of genes encoding TLR4 and TLR4 pathway members TIRAP and IRAK1 are effective on MCP1, IL6, IL1β, and TNFα levels in type 2 diabetes and insulin resistance
      IF: 3.061
      Journal: INFLAMMATION RESEARCH(2019)
      DOI: 10.1007/s00011-019-01263-7
      样本类型: serum
      物种: Human
    15. Inflammatory Biomarkers Levels in T2DM Emirati Patients with Diabetic Neuropathy
      IF: 3.168
      Journal: Diabetes Metabolic Syndrome and Obesity-Targets and Therapy(2021)
      DOI: 10.2147/DMSO.S319863
      PMID: 34345175
      样本类型: serum
      物种: Human
    16. Profiling and functional characterization of circulation LncRNAs that are associated with coronary atherosclerotic plaque stability
      IF: 3.266
      Journal: American Journal of Translational Research(2019)
      PMID: 31312390
      样本类型: THP-1 cells
      物种: Human
    17. Chronic fatigue and fibromyalgia symptoms are key components of deficit schizophrenia and are strongly associated with activated immune-inflammatory pathways
      IF: 3.759
      Journal: SCHIZOPHRENIA RESEARCH(2020)
      DOI: 10.1016/j.schres.2020.05.003
      PMID: 32467068
      样本类型: Serum
      物种: Human
    18. Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs production
      IF: 3.84
      Journal: BIOSCIENCE REPORTS(2019)
      DOI: 10.1042/BSR20191252
      PMID: 31651935
      样本类型: Serum,Plasma,Cell sample
      物种: Human,Mouse,Rat
    19. Effects of a polysaccharide-based multi-ingredient supplement on salivary immunity in non-elite marathon runners
      IF: 3.841
      Journal: Journal of the International Society of Sports Nutrition(2019)
      DOI: 10.1186/s12970-019-0281-z
      样本类型: saliva
      物种: Human
    20. Intermittent hypoxia modulates redox homeostasis, lipid metabolism associated inflammatory processes and redox post-translational modifications: Benefits at high altitude
      IF: 3.998
      Journal: Scientific Reports(2020)
      DOI: 10.1038/s41598-020-64848-x
      PMID: 32404929
      样本类型: Plasma
      物种: Human
    21. Effect of Lycium barbarum polysaccharide supplementation in non-alcoholic fatty liver disease patients: study protocol for a randomized controlled trial
      IF: 2.279
      Journal: Trials(2021)
      DOI: 10.1186/s13063-021-05529-6
      PMID: 34521466
      样本类型: blood
      物种: Human
    22. Downregulation of hsa_circ_0068087 ameliorates TLR4/NF-κB/NLRP3 inflammasome-mediated inflammation and endothelial cell dysfunction in high glucose conditioned by sponging miR-197
      IF: 2.638
      Journal: GENE(2019)
      DOI: 10.1016/j.gene.2019.05.012
      样本类型: Human Umbilical Vein Endothelial Cells (HUVECs)
      物种: Human
    23. Salivary immunity and lower respiratory tract infections in non-elite marathon runners
      IF: 2.766
      Journal: PLoS One(2018)
      DOI: 10.1371/journal.pone.0206059
      PMID: 30462646
      样本类型: saliva
      物种: Human
    24. Depot-specific inflammation with decreased expression of ATM2 in white adipose tissues induced by high-margarine/lard intake
      IF: 2.806
      Journal: PLoS One(2017)
      DOI: 10.1371/journal.pone.0188007
      样本类型: Cell culture supernatant
      物种: Human
    25. The Neuroimmune and Neurotoxic Fingerprint of Major Neurocognitive Psychosis or Deficit Schizophrenia: a Supervised Machine Learning Study
      IF: 2.992
      Journal: NEUROTOXICITY RESEARCH(2020)
      DOI: 10.1007/s12640-019-00112-z
      样本类型: serum
      物种: Human
    26. MicroRNA-124 alleviates hyperoxia-induced inflammatory response in pulmonary epithelial cell by inhibiting TLR4/NF-κB/CCL2
      IF: 1.396
      Journal: International Journal of Clinical and Experimental Pathology(2018)
      PMID: 31938089
      样本类型: Beas2B cells
      物种: Human
    27. Curcumin affects ox‑LDL‑induced IL‑6, TNF‑α, MCP‑1 secretion and cholesterol efflux in THP‑1 cells by suppressing the TLR4/NF‑κB/miR33a signaling pathway
      IF: 1.785
      Journal: Experimental and Therapeutic Medicine(2020)
      DOI: 10.3892/etm.2020.8915
      样本类型: Cell culture supernatant
      物种: Human,Mouse
    28. Serum malondialdehyde, monocyte chemoattractant protein-1, and vitamin C levels in wet type age-related macular degeneration patients:
      Journal: Therapeutic Advances in Ophthalmology(2020)
      DOI: 10.1177/2515841420951682
      样本类型: Serum
      物种: Human
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