Human MMP-9(Matrix Metalloproteinase 9) ELISA Kit
中文名称：人基质金属蛋白酶9(MMP-9)酶联免疫吸附测定试剂盒
MMP9、CLG4B、Gelatinase B、GELB、MANDP2、92kDa Type IV Collagenase、92 KDa Gelatinase
Price:
- 反应性： Human
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人MMP-9抗体包被于酶标板上，实验时样品（或标准品）中的人MMP-9会与包被抗体结合。后依次加入生物素化的抗人MMP-9抗体和辣根过氧化物酶标记的亲和素，抗人MMP-9抗体与结合在包被抗体上的人MMP-9结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MMP-9浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MMP-9的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96T
/ 48T
/ 96T*5
|
| 反应时间 | 3.5h |
| 反应性 | Human |
| 检测方法 | Colormetric |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人MMP-9，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃/-20℃ |
| 数据处理 |
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MMP2/MMP9-mediated CD100 shedding is crucial for inducing intrahepatic anti-HBV CD8 T cell responses and HBV clearance
IF: 18.946 -
Geometrically encoded SERS nanobarcodes for the logical detection of nasopharyngeal carcinoma-related progression biomarkers
IF: 14.919 -
Quantitative phosphoproteomic analysis identifies the critical role of JNK1 in neuroinflammation induced by Japanese encephalitis virus
IF: 7.359Journal：Science Signaling -
The Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases
IF: 6.6Journal：Cells -
Dual-Sensitive Fluorescent Nanoprobes for Detection of Matrix Metalloproteinases and Low pH in 3D Tumor Microenvironment
IF: 6.331Journal：Journal of Materials Chemistry B -
Mechanisms of Antitumor Invasion and Metastasis of the Marine Fungal Derivative Epi-Aszonalenin A in HT1080 Cells
IF: 6.085Journal：Marine Drugs -
Hypermethylation of the Keap1 gene inactivates its function, promotes Nrf2 nuclear accumulation, and is involved in arsenite-induced human keratinocyte transformation
IF: 5.736Journal：FREE RADICAL BIOLOGY AND MEDICINE -
Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo
IF: 5.65Journal：INTERNATIONAL JOURNAL OF ONCOLOGY -
A novel glyceroglycolipid from brown algae Ishige okamurae improve photoaging and counteract inflammation in UVB-induced HaCaT cells
IF: 5.194 -
Infection of Mycobacterium tuberculosis Promotes Both M1/M2 Polarization and MMP Production in Cigarette Smoke-Exposed Macrophages
IF: 5.085
Q1:Can ELISA measure the cell supernatant MMP-2 and MMP-9? I read the papers written by others, in addition to measuring the expression of these two indicators, but also to measure the activity, can ELISA measure the activity? What's the difference with gelatin enzymology?
These two kits can be used for the detection of your cell supernatant. However, ELISA detection of cell supernatants is affected by factors such as media components, cell growth, and external drug stimulation conditions, and it is recommended that you conduct a preliminary experiment before the formal experiment. Gelatin enzyme spectrometry uses the reversible combination of SDS and MMPs in the sample to separate MMP-2 and MMP-9 in the sample, and then restores the activity of both through the bivalent metal ion buffer system. This test method is specifically aimed at the activity detection of MMP-2 and MMP-9. ELISA detects the content (that is, concentration) of the tested substance through the binding reaction of antigen and antibody, that is, captures the MMP-2 and MMP-9 of the sample through the specific antibodies of MMP-2 and MMP-9. The experimental principles of the two are different, and the activity of MMP-2 and MMP-9 cannot be detected by ELISA.
