本公司产品仅供体外研究使用,不用于临床诊断

Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

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  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
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  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
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  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
    产品货号:E-EL-H0109c

    中文名:人肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

    别称:DIF, TNF-alpha, TNFA, TNFSF2

    规格:
    • 96T
    • 48T
    • 96T*5
    数量:
    - +
    价格: ¥2400

    反应性:

    检测范围: 7.81~500 pg/mL

    灵敏度: 4.69 pg/mL

    货期: 3个工作日+顺丰周期

    在线咨询 比较 加入购物车 说明书

    用途

    该试剂盒用于体外定量检测Human 血清,血浆或其他生物体液中天然及部分重组TNF-α浓度

    检测原理

    本试剂盒采用双抗体夹心ELISA法。用抗人TNF-α抗体包被于酶标板上,实验时样品或标准品中的人TNF-α会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗人TNF-α抗体和辣根过氧化物酶标记的亲和素。抗人TNF-α抗体与结合在包被抗体上的人TNF-α结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,TNF-α浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TNF-α的浓度。

    反应类型 夹心法
    规格 96T
    反应时间 3.5h
    反应性 Human
    检测方法 Colormetric
    检测范围 7.81—500 pg/mL
    灵敏度 4.69 pg/mL
    样本体积 100μL
    样本类型 血清,血浆或其他生物体液

    特异性

    可检测样本中的人TNF-α,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average Corrected
    500 2.427
    2.445
    2.436 2.376
    250 1.562
    1.584
    1.573 1.513
    125 0.993
    0.979
    0.986 0.926
    62.5 0.477
    0.515
    0.496 0.436
    31.25 0.245
    0.237
    0.241 0.181
    15.63 0.166
    0.16
    0.163 0.103
    7.81 0.105
    0.121
    0.113 0.053
    0 0.059
    0.061
    0.06 --

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

    Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    24.10 79.50 183.00 22.70 86.50 177.40
    Standard
    deviation
    1.50 3.80 9.70 1.20 4.00 7.60
    C V (%) 6.22 4.78 5.30 5.29 4.62 4.28

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=8) 86-100 92
    EDTA plasma (n=8) 94-106 99
    Cell culture media (n=8) 93-107 99

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 92-105 84-97 88-99
    Average (%) 99 91 93
    1:4 Range (%) 99-116 87-99 91-104
    Average (%) 107 93 98
    1:8 Range (%) 97-113 81-93 92-109
    Average (%) 104 88 99
    1:16 Range (%) 96-112 85-98 95-107
    Average (%) 103 90 100

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2 支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 2-8℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 2-8℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 2-8℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1. 酶标仪(450nm波长滤光片)
    • 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3. 37℃恒温箱,双蒸水或去离子水
    • 4. 吸水纸

    操作步骤

    1. 对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟

    2. 弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟

    3. 弃掉板内液体,洗板3次

    4. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次

    5. 每孔加入90μL底物溶液,37℃孵育15分钟左右

    6. 每孔加入50μL终止液

    7. 立即在450nm波长下读数,处理数据

    客户发表文献

    1. Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output
      IF: 38.637
      Journal: Cell(2020)
      DOI: 10.1016/j.cell.2020.08.009
      样本类型: Cell culture medium
      物种: Human
    2. Increased Liver Localization of Lipopolysaccharides in Human and Experimental NAFLD
      IF: 14.679
      Journal: Hepatology(2020)
      DOI: 10.1002/hep.31056
      PMID: 31808577
      样本类型: Serum
      物种: Human
    3. Overcoming The Heat Endurance Of Tumor Cells By Interfering The Anaerobic Glycolysis Metabolism For Improved Photothermal Therapy.
      IF: 13.942
      Journal: Acs Nano(2017)
      DOI: 10.1021/acsnano.6b06658
      PMID: 28107631
      样本类型: Cell culture supernatant
      物种: Human
    4. A SCID mouse-human lung xenograft model of SARS-CoV-2 infection
      IF: 11.556
      Journal: Theranostics(2021)
      DOI: 10.7150/thno.58321
      PMID: 33995679
      样本类型: Tissue homogenate
      物种: Human
    5. Circular RNA circACSL1 aggravated myocardial inflammation and myocardial injury by sponging miR-8055 and regulating MAPK14 expression
      IF: 8.4689
      Journal: Cell Death & Disease(2021)
      DOI: 10.1038/s41419-021-03777-7
      PMID: 33986259
      样本类型: Cell culture supernatant
      物种: Human
    6. Dual functionalized chitosan based composite hydrogel for haemostatic efficacy and adhesive property
      IF: 7.182
      Journal: Carbohydrate Polymers(2020)
      DOI: 10.1016/j.carbpol.2020.116757
      PMID: 32829870
      样本类型: Cell culture supernatant
      物种: Human
    7. Exploiting A New Strategy To Induce Immunogenic Cell Death To Improve Dendritic Cell-Based Vaccines For Lymphoma Immunotherapy.
      IF: 7.719
      Journal: OncoImmunology(2017)
      DOI: 10.1080/2162402X.2017.1356964
      PMID: 29147614
      样本类型: Cell culture supernatant
      物种: Human
    8. Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity
      IF: 6.529
      Journal: Biomedicine & Pharmacotherapy(2021)
      DOI: 10.1016/j.biopha.2021.111531
      PMID: 34311530
      样本类型: Serum
      物种: Human,Mouse
    9. Green coffee extract modifies body weight, serum lipids and TNF-α in high-fat diet-induced obese rats
      IF: 6.782
      Journal: BMC Research Notes(2020)
      DOI: 10.1186/s13104-020-05052-y
      PMID: 32276657
      样本类型: Serum
      物种: Rat
    10. The enhancer RNA lnc-SLC4A1-1 epigenetically regulates unexplained recurrent pregnancy loss (URPL) by activating CXCL8 and NF-kB pathway
      IF: 6.183
      Journal: EBioMedicine(2018)
      DOI: 10.1016/j.ebiom.2018.11.015
      PMID: 30448228
      样本类型: Cell culture supernatant
      物种: Human
    11. Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils
      IF: 5.546
      Journal: Journal of Cellular Physiology(2020)
      DOI: 10.1002/jcp.29713
      PMID: 32385929
      样本类型: Cell culture supernatant
      物种: Human
    12. Fontan protein-losing enteropathy is associated with advanced liver disease and a proinflammatory intestinal and systemic state
      IF: 5.175
      Journal: Liver International(2020)
      DOI: 10.1111/liv.14375
      PMID: 31912956
      样本类型: Plasma
      物种: Human
    13. Effects of circulating extracellular microvesicles from spinal cord-injured adults on endothelial cell function
      IF: 5.223
      Journal: Clinical Science(2020)
      DOI: 10.1042/CS20200047
      PMID: 32219341
      样本类型: Cell culture supernatant
      物种: Human
    14. Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis.
      IF: 5.454
      Journal: Osteoarthritis and cartilage(2018)
      DOI: 10.1016/j.joca.2018.05.003
      PMID: 29753949
      样本类型: Serum
      物种: Human
    15. Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output
      IF: 4.966
      Journal: The FASEB Journal(2020)
      DOI: 10.1016/j.cell.2020.08.009
      PMID: 32931733
      样本类型: Cell culture supernatant
      物种: Human
    16. Inhibition of TLR2/TLR4 alleviates the Neisseria gonorrhoeae infection damage in human endometrial epithelial cells via Nrf2 and NF-Kβsignaling
      IF: 4.018
      Journal: Journal of Reproductive Immunology(2020)
      DOI: 10.1016/j.jri.2020.103192
      PMID: 32950783
      样本类型: Cell culture supernatant
      物种: Human
    17. LncRNA SNHG5 regulates cell apoptosis and inflammation by miR-132/PTEN axis in COPD
      IF: 4.545
      Journal: Biomedicine & Pharmacotherapy(2020)
      DOI: 10.1016/j.biopha.2020.110016
      PMID: 32145584
      样本类型: Cell culture supernatant
      物种: Human
    18. Long non-coding RNA NNT-AS1 regulates proliferation, apoptosis, inflammation and airway remodeling of chronic obstructive pulmonary disease via targeting miR-582-5p/FBXO11 axis
      IF: 4.545
      Journal: Biomedicine & Pharmacotherapy(2020)
      DOI: 10.1016/j.biopha.2020.110326
      样本类型: Cell culture supernatant
      物种: Human
    19. Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals
      IF: 4.234
      Journal: Diabetes research and clinical practice(2019)
      DOI: 10.1016/j.diabres.2019.05.023
      PMID: 31150725
      样本类型: Serum
      物种: Human
    20. Inflammation-dependent ISG15 upregulation mediates MIA-induced dendrite damages and depression by disrupting NEDD4/Rap2A signaling
      IF: 4.328
      Journal: Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease(2019)
      DOI: 10.1016/j.bbadis.2019.02.020
      PMID: 30826466
      样本类型: Serum
      物种: Human,Rat
    21. Zinc Oxide Nanoparticle-Induced Atherosclerotic Alterations In Vitro And In Vivo.
      IF: 4.3
      Journal: International Journal of Nanomedicine(2017)
      DOI: 10.2147/IJN.S134897
      PMID: 28652743
      样本类型: Cell culture supernatant,Serum
      物种: Human,Rat
    22. Ros-Dependent Activation Of Autophagy Through The PI3K/Akt/mTOR Pathway Is Induced By Hydroxysafflor Yellow A-Sonodynamic Therapy In THP-1 Macrophages.
      IF: 4.593
      Journal: Oxidative Medicine and Cellular Longevity(2017)
      DOI: 10.1155/2017/8519169
      PMID: 28191279
      样本类型: Cell culture supernatant
      物种: Human
    23. The Role Of miR-19b In The Inhibition Of Endothelial Cell Apoptosis And Its Relationship With Coronary Artery Disease.
      IF: 4.259
      Journal: Scientific Reports(2015)
      DOI: 10.1038/srep15132
      PMID: 26459935
      样本类型: Serum
      物种: Human
    24. Multifunctional Dextran Sulfate-Coated Reconstituted High Density Lipoproteins Target Macrophages And Promote Beneficial Antiatherosclerotic Mechanisms.
      IF: 4.818
      Journal: Bioconjugate Chemistry(2016)
      DOI: 10.1021/acs.bioconjchem.6b00600
      PMID: 28004910
      样本类型: Cell culture supernatant
      物种: Human
    25. Positive Feedback Cycle Of TNF-α Promotes Staphylococcal Enterotoxin B-Induced THP-1 Cell Apoptosis.
      IF: 4.3
      Journal: Frontier in Cellular and Infection Microbiology(2016)
      DOI: 10.3389/fcimb.2016.00109
      PMID: 27709104
      样本类型: Cell culture supernatant
      物种: Human
    26. Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits
      IF: 3.998
      Journal: Scientific reports(2020)
      DOI: 10.1038/s41598-020-68543-9
      PMID: 32669704
      样本类型: Cell culture supernatant
      物种: Human
    27. Molecular characterization, inducible expression and functional analysis of an IKKβ from the tropical sea cucumber Holothuria leucospilota
      IF: 3.298
      Journal: Fish & Shellfish Immunology(2020)
      DOI: 10.1016/j.fsi.2020.06.024
      PMID: 32585358
      样本类型: Cell culture supernatant
      物种: Human
    28. iTRAQ-based proteomics and in vitro experiments reveals essential roles of ACE and AP-N in the renin-angiotensin system-mediated congenital ureteropelvic junction obstruction
      IF: 3.263
      Journal: European Journal of Pharmacology(2020)
      DOI: 10.1016/j.yexcr.2020.112086
      PMID: 32416091
      样本类型: Serum,Cell sample
      物种: Human,Mouse
    29. Anti-angiogenic effect of Shikonin in rheumatoid arthritis by downregulating PI3K/AKT and MAPKs signaling pathways
      IF: 3.69
      Journal: Journal of Ethnopharmacology(2020)
      DOI: 10.1016/j.jep.2020.113039
      PMID: 32497675
      样本类型: Serum,Cell culture supernatant
      物种: Human,Rat
    30. miR-942-5p alleviates septic acute kidney injury by targeting FOXO3
      IF: 3.024
      Journal: European review for medical and pharmacological sciences(2020)
      DOI: 10.26355/eurrev_202006_21521
      PMID: 32572890
      样本类型: Cell culture supernatant
      物种: Human
    31. Notopterygium incisum extract (NRE) rescues cognitive deficits in APP/PS1 Alzhneimer's disease mice by attenuating amyloid-beta, tau, and neuroinflammation pathology
      IF: 3.69
      Journal: Journal of Ethnopharmacology(2020)
      DOI: 10.1016/j.jep.2019.112433
      PMID: 31783135
      样本类型: Cell culture supernatant
      物种: Human
    32. Tocilizumab attenuates acute lung and kidney injuries and improves survival in a rat model of sepsis via down-regulation of NF-κB/JNK: a possible role of P-glycoprotein
      IF: 3.238
      Journal: Inflammopharmacology(2020)
      DOI: 10.1007/s10787-019-00628-y
      PMID: 31440860
      样本类型: Serum
      物种: Rat
    33. Perillaldehyde Ameliorates Aspergillus fumigatus Keratitis by Activating the Nrf2/HO-1 Signaling Pathway and Inhibiting Dectin-1-Mediated Inflammation
      IF: 3.47
      Journal: Investigative ophthalmology & visual science(2020)
      DOI: 10.1167/iovs.61.6.51
      PMID: 32579678
      样本类型: Cell lysate,Cell culture medium
      物种: Human
    34. Chronic fatigue and fibromyalgia symptoms are key components of deficit schizophrenia and are strongly associated with activated immune-inflammatory pathways
      IF: 3.759
      Journal: Schizophrenia Research(2020)
      DOI: 10.1016/j.schres.2020.05.003
      PMID: 32467068
      样本类型: Serum
      物种: Human
    35. Plasma concentration of ZCCHC14 contributes to prognostic efficacy in intracerebral hemorrhage patients
      IF: 3.024
      Journal: European review for medical and pharmacological sciences(2019)
      DOI: 10.26355/eurrev_201910_19167
      PMID: 31646585
      样本类型: Plasma
      物种: Human
    36. miR-A-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis
      IF: 3.967
      Journal: Apoptosis(2018)
      DOI: 10.1007/s10495-018-1474-7
      PMID: 30167920
      样本类型: Serum
      物种: Human,Rat
    37. S1P promotes inflammation-induced tube formation by HLECs via the S1PR1/NF-κB pathway
      IF: 3.118
      Journal: International immunopharmacology(2019)
      DOI: 10.1016/j.intimp.2018.11.032
      PMID: 30476824
      样本类型: Cell culture supernatant
      物种: Human
    38. LncRNA PMS2L2 protects ATDC5 chondrocytes against lipopolysaccharide-induced inflammatory injury by sponging miR-203
      IF: 3.234
      Journal: Life sciences(2019)
      DOI: 10.1016/j.lfs.2018.12.020
      PMID: 30550887
      样本类型: Cell culture supernatant
      物种: Human
    39. IL-16 regulates macrophage polarization as a target gene of miR-145-3p
      IF: 3.188
      Journal: Molecular immunology(2019)
      DOI: 10.1016/j.molimm.2018.12.027
      PMID: 30634164
      样本类型: Cell lysate
      物种: Human
    40. Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro
      IF: 3.457
      Journal: Biomedicine & Pharmacotherapy(2019)
      DOI: 10.1016/j.biopha.2019.108706
      PMID: 30844656
      样本类型: Wound drainage fluid
      物种: Human
    41. Biochemical investigation of gender-specific association between insulin resistance and inflammatory biomarkers in types 2 diabetic patients.
      IF: 3.457
      Journal: Biomedicine & Pharmacotherapy(2018)
      DOI: 10.1016/j.biopha.2018.06.044
      PMID: 29966972
      样本类型: Serum
      物种: Human
    42. Short-Term Postoperative Cognitive Dysfunction And Inflammatory Response In Patients Undergoing Cytoreductive Surgery And Hyperthermic Intraperitoneal Chemotherapy: A Pilot Study.
      IF: 3.232
      Journal: Mediators Of Inflammation(2017)
      DOI: 10.1155/2017/3605350
      PMID: 29249869
      样本类型: Serum
      物种: Human
    43. The Pharmaceutical Device Prisma Skin Promotes In Vitro Angiogenesis Through Endothelial To Mesenchymal Transition During Skin Wound Healing.
      IF: 3.226
      Journal: International Journal of Molecular Sciences(2017)
      DOI: 10.3390/ijms18081614
      PMID: 28757565
      样本类型: Cell culture supernatant
      物种: Human
    44. In Vitro Antiproliferative, Pro-Apoptotic, Antimetastatic And Anti-Inflammatory Potential Of 2, 4-Diacteylphloroglucinol (DAPG) By Pseudomonas Aeruginosa Strain FP10.
      IF: 3.833
      Journal: Apoptosis(2015)
      DOI: 10.1007/s10495-015-1162-9
      PMID: 26283170
      样本类型: Cell culture supernatant
      物种: Human
    45. Inhibitory Effect Of miR-125b On Hepatitis C Virus Core Protein-Induced TLR2/MyD88 Signaling In THP-1 Macrophages.
      IF: 3.365
      Journal: World Journal of Gastroenterology(2016)
      DOI: 10.3748/wjg.v22.i17.4354
      PMID: 27158204
      样本类型: Cell culture supernatant
      物种: Human
    46. Intermittent hypoxia induces tumor immune escape in murine S180 solid tumors via the upregulation of TGF-β 1 in mice
      IF: 2.162
      Journal: Sleep and Breathing(2020)
      DOI: 10.1007/s11325-020-02166-2
      PMID: 32840731
      样本类型: Tissue homogenate
      物种: Mouse
    47. Obstructive sleep apnea in children with hypothalamic obesity: Evaluation of possible related factors
      IF: 2.534
      Journal: Pediatric Pulmonology(2020)
      DOI: 10.1002/ppul.25097
      PMID: 32986303
      样本类型: Plasma
      物种: Human
    48. Investigation of DNA damage, oxidative stress, and inflammation in synthetic cannabinoid users
      IF: 2.067
      Journal: Human & Experimental Toxicology(2020)
      DOI: 10.1177/0960327120930057
      PMID: 32508150
      样本类型: Plasma
      物种: Human
    49. Auto-reactivity against gut bacterial peptides in patients with late-onset diabetes
      IF: 2.125
      Journal: Autoimmunity(2020)
      DOI: 10.1080/08916934.2020.1818232
      PMID: 32924613
      样本类型: Serum
      物种: Human
    50. XIST silencing alleviated inflammation and mesangial cells proliferation in diabetic nephropathy by sponging miR-485
      IF: 2.575
      Journal: Archives of Physiology and Biochemistry(2020)
      DOI: 10.1080/13813455.2020.1789880
      PMID: 32669002
      样本类型: Serum
      物种: Human
    51. Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China
      IF: 2.082
      Journal: Journal of Zhejiang University. Science. B(2020)
      DOI: 10.1631/jzus.B2000174
      PMID: 32425003
      样本类型: Serum,Plasma
      物种: Human
    52. Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line
      IF: 2.639
      Journal: Biological Trace Element Research(2019)
      DOI: 10.1007/s12011-019-01831-2
      PMID: 31473895
      样本类型: Cell lysate
      物种: Human
    53. Long non-coding RNA MEG3 regulates CSE-induced apoptosis and inflammation via regulating miR-218 in 16HBE cells
      IF: 2.985
      Journal: Biochemical and Biophysical Research Communications(2020)
      DOI: 10.1016/j.bbrc.2019.10.135
      PMID: 31668807
      样本类型: Cell culture supernatant
      物种: Human
    54. Early HIV infection is associated with reduced proportions of gamma delta T subsets as well as high creatinine and urea levels
      IF: 2.717
      Journal: Scandinavian Journal of Immunology(2020)
      DOI: 10.1111/sji.12868
      PMID: 32052490
      样本类型: Serum
      物种: Human
    55. Effect of Helicobacter pylori-eradication therapy on hepatic steatosis in patients with non-alcoholic fatty liver disease: a randomized-controlled pilot study
      IF: 2.96
      Journal: Gastroenterology Report(2020)
      DOI: 10.1093/gastro/goz058
      PMID: 32499918
      样本类型: Serum
      物种: Human
    56. The effect of different dietary ratios of arginine, methionine, and lysine on the performance, carcass traits, and immune status of turkeys
      IF: 2.659
      Journal: Poultry Science(2020)
      DOI: 10.1016/j.psj.2019.10.008
      PMID: 32036960
      样本类型: Plasma
      物种: Chicken
    57. Major Depression in Children with Transfusion-Dependent Thalassemia Is Strongly Associated with the Combined Effects of Blood Transfusion Rate, Iron Overload, and Increased pro-inflammatory Cytokines
      IF: 2.992
      Journal: Neurotoxicity research(2020)
      DOI: 10.1007/s12640-020-00193-1
      PMID: 32335809
      样本类型: Serum
      物种: Human
    58. Isolation, characterization and analysis of pro-inflammatory potential of Klebsiella pneumoniae outer membrane vesicles
      IF: 2.914
      Journal: Microbial pathogenesis(2019)
      DOI: 10.1016/j.micpath.2019.103719
      PMID: 31493501
      样本类型: Cell culture supernatant
      物种: Human
    59. Autoantibodies to a Specific Peptide Epitope of Human Hsp60 (ATVLA) With Homology to Helicobacter Pylori HspB in H. Pylori-Infected Patients
      IF: 2.026
      Journal: Apmis(2019)
      DOI: 10.1111/apm.12925
      PMID: 30746790
      样本类型: Cell culture supernatant
      物种: Human
    60. In Vitro Immunomodulatory Effect of Food Supplement from Aloe vera
      IF: 2.064
      Journal: Evidence-Based Complementary and Alternative Medicine(2019)
      DOI: 10.1155/2019/5961742
      PMID: 30941196
      样本类型: Cell culture supernatant
      物种: Human
    61. Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1-7) upregulation.
      IF: 2.759
      Journal: Biomedicine & Pharmacotherapy(2018)
      DOI: 10.1016/j.biopha.2017.11.151
      PMID: 29793333
      样本类型: Cell culture supernatant,Serum
      物种: Human,Rabbit
    62. High Anti-Human Cytomegalovirus Antibody Levels Are Associated With The Progression Of Essential Hypertension And Target Organ Damage In Han Chinese Population.
      IF: 2.806
      Journal: Plos One(2017)
      DOI: 10.1371/journal.pone.0181440
      PMID: 28837559
      样本类型: Serum
      物种: Human
    63. C Reactive Protein And Enzymatically Modified LDL Cooperatively Promote Dendritic Cell-Mediated T Cell Activation.
      IF: 2.359
      Journal: Cardiovascular Pathology(2017)
      DOI: 10.1016/j.carpath.2017.03.009
      PMID: 28433861
      样本类型: Cell culture supernatant
      物种: Human
    64. Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation Through Both ROS Scavenging And Inhibition Of Cholesterol Influx In Raw264.7 Cells.
      IF: 2.861
      Journal: Molecules(2015)
      DOI: 10.3390/molecules201219764
      PMID: 26633327
      样本类型: Cell lysate
      物种: Human
    65. Sublethal Effects Of Zinc Oxide Nanoparticles On Male Reproductive Cells.
      IF: 2.866
      Journal: Toxicology in Vitro(2016)
      DOI: 10.1016/j.tiv.2016.05.017
      PMID: 27247145
      样本类型: Cell culture supernatant
      物种: Human
    66. Decreased Monocyte Subsets And TLR4-Mediated Functions In Patients With Acute Severe Fever With Thrombocytopenia Syndrome (SFTS).
      IF: 2.532
      Journal: International Journal of Infectious Diseases(2015)
      DOI: 10.1016/j.ijid.2015.12.009
      PMID: 26701820
      样本类型: Cell culture supernatant
      物种: Human
    67. Oxidative Burst Of Circulating Neutrophils Following Traumatic Brain Injury In Human.
      IF: 2.806
      Journal: Plos One(2013)
      DOI: 10.1371/journal.pone.0068963
      PMID: 23894384
      样本类型: Plasma
      物种: Human
    68. Rhubarb-Aconite Decoction (RAD) Drug-Containing Serum Alleviated Endotoxin-Induced Oxidative Stress Injury and Inflammatory Response in Caco-2 Cells In Vitro
      IF: 1.813
      Journal: Evidence-Based Complementary and Alternative Medicine(2020)
      DOI: 10.1155/2020/5834502
      PMID: 32714409
      样本类型: Cell culture supernatant
      物种: Human
    69. Nec-1 attenuates inflammation and cytotoxicity induced by high glucose on THP-1 derived macrophages through RIP1
      IF: 1.931
      Journal: Archives of Oral Biology(2020)
      DOI: 10.1016/j.archoralbio.2020.104858
      PMID: 32805637
      样本类型: Cell culture supernatant
      物种: Human
    70. Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF-α-mediated osteoclast autophagy
      IF: 1.785
      Journal: Experimental and therapeutic medicine(2020)
      DOI: 10.3892/etm.2020.8903
      PMID: 32782497
      样本类型: Cell culture supernatant
      物种: Human
    71. Correlation Between Single Nucleotide Polymorphisms at the 3'-UTR of the NFKB1 Gene and Acute Kidney Injury in Sepsis
      IF: 1.346
      Journal: Genetic Testing and Molecular Biomarkers(2020)
      DOI: 10.1089/gtmb.2019.0222
      PMID: 32315555
      样本类型: Cell sample
      物种: Human
    72. Avicularin Reduces the Expression of Mediators of Inflammation and Oxidative Stress in Bradykinin-Treated MG-63 Human Osteoblastic Osteosarcoma Cells
      IF: 1.918
      Journal: Medical Science Monitor(2020)
      DOI: 10.12659/MSM.921957
      PMID: 32463805
      样本类型: Cell culture supernatant
      物种: Human
    73. Systematically transplanted human gingiva-derived mesenchymal stem cells regulate lipid metabolism and inflammation in hyperlipidemic mice with periodontitis
      IF: 1.785
      Journal: Experimental and Therapeutic Medicine(2020)
      DOI: 10.3892/etm.2019.8256
      PMID: 31885706
      样本类型: Serum
      物种: Human
    74. Effect of laparoscopic sleeve gastrectomy on renal function in obese patients
      IF: 1.355
      Journal: ANZ Journal of Surgery(2020)
      DOI: 10.1111/ans.15767
      PMID: 32115876
      样本类型: Serum,Urine
      物种: Human
    75. A label-free and sensitive impedimetric immunosensor for TNF-α biomarker detection based on epoxysilane-modified disposable ITO-PET electrode
      IF: 1.431
      Journal: International Journal of Environmental Analytical Chemistry(2020)
      DOI: 10.1080/03067319.2019.1679807
      样本类型: Immunosensor Construction
      物种: Non-living
    76. A case-control study on the relationship between miR-As single nucleotide polymorphisms and sepsis risk
      IF: 1.552
      Journal: medicine(2019)
      DOI: 10.1097/MD.0000000000016744
      PMID: 31415369
      样本类型: Serum
      物种: Human
    77. Association of High Mobility Group Box Protein B1 Gene Polymorphisms with Pneumonia Susceptibility and Severity
      IF: 1.181
      Journal: Genetic testing and molecular biomarkers(2018)
      DOI: 10.1089/gtmb.2018.0174
      PMID: 30562142
      样本类型: Plasma
      物种: Human
    78. Mitochondrial DNA-Induced Inflammatory Responses and Lung Injury in Thermal Injury Murine Model: Protective Effect of Cyclosporine-A
      IF: 1.923
      Journal: Journal of Burn Care & Research(2019)
      DOI: 10.1093/jbcr/irz029
      PMID: 30926991
      样本类型: Serum
      物种: Mouse
    79. Anti-inflammatory Effect of Resolvin D1 on LPS-treated MG-63 Cells
      IF: 1.41
      Journal: Experimental and therapeutic medicine(2018)
      DOI: 10.3892/etm.2018.6721
      PMID: 30402165
      样本类型: Serum
      物种: Human
    80. Effects of propofol combined with remifentanil anesthesia on the NO, endothelin and inflammatory cytokines in the plasma of patients with liver cirrhosis during the perioperative period
      IF: 1.41
      Journal: Experimental and Therapeutic Medicine(2019)
      DOI: 10.3892/etm.2019.7367
      PMID: 30988754
      样本类型: Plasma
      物种: Human
    81. Leukocyte Depletion Does Not Affect The In Vitro Healing Ability Of Platelet Rich Plasma.
      IF: 1.785
      Journal: Experimental and Therapeutic Medicine(2018)
      DOI: 10.3892/etm.2018.5887
      PMID: 29556269
      物种: Human
    82. Tetrahydroxy Stilbene Glucoside Ameliorates H2O2-Induced Human Brain Microvascular Endothelial Cell Dysfunction In Vitro By Inhibiting Oxidative Stress And Inflammatory Responses.
      IF: 1.692
      Journal: Molecular Medicine Reports(2017)
      DOI: 10.3892/mmr.2017.7225
      PMID: 28849141
      样本类型: Cell culture supernatant
      物种: Human
    83. The Shortening Telomere Length Of T Lymphocytes Maybe Associated With HyperFunction In Servere Aplastic Anemia.
      IF: 1.692
      Journal: Molecular Medicine Reports(2018)
      DOI: 10.3892/mmr.2017.8014
      PMID: 29115638
      物种: Human
    84. Role Of miR-589-3p In Human Lumbar Disc Degeneration And Its Potential Mechanism.
      IF: 1.261
      Journal: Experimental and Therapeutic Medicine(2018)
      DOI: 10.3892/etm.2017.5593
      PMID: 29434746
      样本类型: Cell culture supernatant
      物种: Human
    85. Inhibition Of miR27a Suppresses The Inflammatory Response Via The p38/MAPK Pathway In Intervertebral Disc Cells.
      IF: 1.261
      Journal: Experimental and Therapeutic Medicine(2017)
      DOI: 10.3892/etm.2017.5053
      PMID: 29067127
      样本类型: Cell culture supernatant
      物种: Human
    86. Human cytomegalovirus infection is correlated with atherosclerotic plaque vulnerability in carotid artery
      Journal: The Journal of Gene Medicine(2020)
      DOI: 10.1002/jgm.3236
      PMID: 32468600
      样本类型: Serum
      物种: Human
    87. The role of TNF and Resistin Gene+ 299 (G/A) polymorphism in the development of insulin resistance in non-obese Type 2 Diabetes Mellitus Iraqi patients
      Journal: Int J Curr Microbiol App Sci(2015)
      DOI: 99999999
      样本类型: Serum
      物种: Human
    88. Microbial Index and TNF-α, IL-4, CCL17 Level Among Burn Wound Infections in Hilla City, Iraq.
      Journal: Biomedical and Pharmacology Journal(2016)
      DOI: 10.13005/bpj/1054
      样本类型: Serum
      物种: Human
    89. The activation, ROS induction and cytokine secretion in human monocytes exposed to H . pylori lipopolysaccharide depend on the availability of TLR4/2 and TLR2/6 receptor complexes
      Journal: European Microscopy Congress 2016: Proceedings(2016)
      DOI: 10.1002/9783527808465.EMC2016.6255
      样本类型: Cell culture supernatant
      物种: Human
    90. Evaluation of Cytokines Response in Plasmodium falciparum Infection among Adolescents in Port Harcourt, Nigeria
      Journal: JMSCR(2018)
      DOI: 10.18535/jmscr/v6i9.54
      样本类型: Plasma
      物种: Human
    91. The neuro-immune fingerprint of major neuro-cognitive psychosis or deficit schizophrenia: a supervised machine learning study
      Journal: Preprint(2019)
      DOI: 10.20944/preprints201905.0285.v1
      样本类型: Serum
      物种: Human
    92. Influence of TLR4 Polymorphisms on Susceptibility to UTI Caused by Gram Negative Bacteria in Pregnant Women
      Journal: Indian Journal of Forensic Medicine & Toxicology(2019)
      DOI: 10.5958/0973-9130.2019.00454.7
      样本类型: Serum,Urine
      物种: Human
    93. TNFR, TRAF2, NF-κB mRNA Levels of Glioblastoma Multiforme Cells Treated by Conditioned Medium of Umbilical Cord-derived Mesenchymal Stem Cells
      Journal: The Indonesian Biomedical Journal(2019)
      DOI: 10.18585/inabj.v11i2.722
      样本类型: Cell lysate
      物种: Human
    94. Correlation of Small Dense Low Density Lipoprotein, Tumour Necrosis Factor-alpha with Liver Enzymes in Chronic Hepatitis B Patients
      Journal: International Journal of Tropical Disease & Health(2019)
      DOI: 10.9734/ijtdh/2019/v37i430172
      样本类型: Serum
      物种: Human
    95. Association of TLR4 Polymorphisms with Susceptibility to Urinary Tract Infection Caused by Gram Positive Bacteria in Pregnant Women
      Journal: Indian Journal of Public Health(2020)
      DOI: 99999999
      样本类型: Serum
      物种: Human
    96. Estimation of IL-1βand TNF Concentration in Children Suffering from Febrile Convulsion
      Journal: The Journal of Research on the Lepidoptera(2020)
      DOI: 10.36872/LEPI/V51I2/301090
      样本类型: Serum
      物种: Human
    97. Thymosin β10 promotes tumor-associated macrophages M2 conversion and proliferation via the PI3K/Akt pathway in lung adenocarcinoma
      Journal: Preprint(2020)
      DOI: 10.21203/rs.3.rs-34263/v1
      样本类型: Cell culture supernatant
      物种: Human
    98. CALML6 Controls TAK1 Ubiquitination and Confers Protection against Acute Inflammation
      Journal: The Journal of Immunology(2020)
      DOI: 10.4049/jimmunol.1901042
      PMID: 32303555
      样本类型: Cell culture supernatant
      物种: Human
    99. Evaluation of Synergistic Effect of Cytosine-PhosphodiesterbondGuanosin-Oligodeoxynucleotide 7909, and Protamine on Transfection Process Mediated by Calcium Phosphate Nanoparticles
      Journal: International Journal of Drug Delivery Technology(2020)
      DOI: 99999999
      样本类型: Cell culture supernatant
      物种: Human
    100. Lowered serum cesium levels in schizophrenia: association with immune-inflammatory biomarkers and cognitive impairments
      Journal: Preprints(2020)
      DOI: 10.1590/1516-4446-2020-0908
      PMID: 32556004
      样本类型: Serum
      物种: Human
    101. Hyperhomocysteinemia and Folate Levels in Normal Healthy Nigerians Living in Zaria: Subanalysis of ABU Homocysteine Cross-sectional Survey
      Journal: Annals of African Medicine(2019)
      DOI: 10.4103/aam.aam_53_18
      PMID: 31417015
      样本类型: Plasma
      物种: Human
    102. TLR7 mediates increased vulnerability to ischemic acute kidney injury in diabetes
      Journal: Revista da Associa??o Médica Brasileira(2019)
      DOI: 10.1590/1806-9282.65.8.1067
      PMID: 31531603
      样本类型: Cell culture supernatant
      物种: Human
    103. Impact of an Early Oral Feeding Protocol on Inflammatory Cytokine Changes After Esophagectomy
      Journal: The Annals of thoracic surgery(2019)
      DOI: 10.1016/j.athoracsur.2018.09.048
      PMID: 30403976
      样本类型: Serum
      物种: Human
    104. Estimation of Cytokines Involved in Acute-Phase Wound Infection with Reference to Residence Time of Patients in Hospitals
      Journal: Modern Research in Inflammation(2019)
      DOI: 10.4236/mri.2019.81001
      样本类型: Serum
      物种: Human
    105. Resveratrol protects human renal proximal tubular cells from high glucose and hypoxia/reoxygenation induced injury via inhibiting p38-MAPK and thioredoxin-interacting protein (TXNIP) pathways
      IF: 0.181
      Journal: NTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE(2019)
      DOI: 99999999
      样本类型: Cell culture supernatant
      物种: Human
    106. Effect of General and Spinal Anesthesia on Some Anti-inflammatory cytokines and cell adhesion molecules of Iraqi Women Undergo Cesarean Section: Comparative Study
      Journal: Journal of Pharmaceutical Sciences and Research(2018)
      DOI: 99999999
      样本类型: Serum
      物种: Human
    107. Sinomenine Regulates Inflammatory Response and Oxidative Stress via Nuclear Factor kappa B (NF-kB) and NF-E2-Related Factor 2 (Nrf2) Signaling Pathways in Ankle Fractures in Children
      Journal: Medical science monitor: international medical journal of experimental and clinical research(2018)
      DOI: 10.12659/MSM.910740
      PMID: 30237391
      样本类型: Serum
      物种: Human
    108. EFFECTS OF GINKGO BILOBA EXTRACT (EGb761) AS ADDON WITH METFORMIN IN THE TREATMENT OF PATIENTS
      Journal: University of Sulaimani(2018)
      DOI: 99999999
      样本类型: Serum
      物种: Human
    109. Tumor Necrosis Factor Alpha Synergetic with Insulin Resistance Potentially Contributes to the Development of Polycystic Ovary Syndrome.
      Journal: Medical Journal of Babylon(2017)
      样本类型: Serum
      物种: Human
    110. Potential Benefit of Melatonin as an Adjuvant Therapy in a Sample of Iraqi Patients with Crohn’s Disease: A New Study.
      Journal: ALMUSTANSIRIYAH Journal of PHARMACEUTICAL SCIENCES (AJPS)(2018)
      样本类型: Serum
      物种: Human
    111. Serum Tnfα Levels Are Inversely Associated With Insulin Sensitivity in Diabetic Retinopathy Patients.
      Journal: Kerbala Jorunal of Medicine(2017)
      样本类型: Serum
      物种: Human
    112. Propofol Promotes Ankle Fracture Healing in Children by Inhibiting Inflammatory Response.
      Journal: Medical Science Monitor: International Medical Journal of Experimental And Clinical Research(2018)
      DOI: 10.12659/MSM.908592
      PMID: 29940605
      样本类型: Serum
      物种: Human
    113. High-Mobility Group Protein Box 1 is Upregulated in Children with Henoch-Schonlein Purpura.
      IF: 0.533
      Journal: Pediatric Allergy(2018)
      DOI: 10.1089/ped.2018.0872
      样本类型: Serum
      物种: Human
    114. Adiponectin may be used as a marker in prediction of diabetic retinopathy patients.
      Journal: Iraq Medical Journal(2018)
      样本类型: Serum
      物种: Human
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