Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
中文名称:人肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.69 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人TNF-α抗体包被于酶标板上,实验时样品(或标准品)中的人TNF-α会与包被抗体结合。后依次加入生物素化的抗人TNF-α抗体和辣根过氧化物酶标记的亲和素,抗人TNF-α抗体与结合在包被抗体上的人TNF-α结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,TNF-α浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TNF-α的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人TNF-α,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Tet methylcytosine dioxygenase 2 (TET2) deficiency elicits EGFR-TKI (tyrosine kinase inhibitors) resistance in non-small cell lung cancer
IF: 39.300 -
Dietary-timing-induced gut microbiota diurnal oscillations modulate inflammatory rhythms in rheumatoid arthritis
IF: 27.700 -
Controlling Alveolar Bone Loss by Hydrogel-Based Mitigation of Oral Dysbiosis and Bacteria-Triggered Proinflammatory Immune Response
IF: 18.500Journal: ADVANCED FUNCTIONAL MATERIALS (2024)Reactivity:HumanSample Type:soft gingival tissue -
Enhancing Glioblastoma Immunotherapy with Integrated Chimeric Antigen Receptor T Cells through the Re-Education of Tumor-Associated Microglia and Macrophages
IF: 15.800 -
Extracellular vesicle-packaged lncRNA from cancer-associated fibroblasts promotes immune evasion by downregulating HLA-A in pancreatic cancer
IF: 15.500 -
H3K4me3-Mediated FOXJ2/SLAMF8 Axis Aggravates Thrombosis and Inflammation in β2GPI/Anti-β2GPI-Treated Monocytes
IF: 14.300 -
Tungsten-rosmarinic acid nanoenzyme with SOD mimetic activity for the treatment of psoriasis by eliminating ROS and relieving inflammation
IF: 13.300 -
Central carbon metabolism exhibits unique characteristics during the handling of fungal patterns by monocyte-derived dendritic cells
IF: 10.700 -
Repair spinal cord injury with a versatile anti-oxidant and neural regenerative nanoplatform
IF: 10.600 -
CircARCN1 aggravates atherosclerosis by regulating HuR-mediated USP31 mRNA in macrophages
IF: 10.200