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Human VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit

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    产品货号:E-EL-H0111c

    中文名:人血管内皮细胞生长因子(VEGF-A)酶联免疫吸附测定试剂盒

    别称:VEGF-A, VEGFA, MVCD1, VPF

    规格:
    • 96T
    • 48T
    数量:
    - +
    价格: ¥2400

    反应性:

    检测范围: 31.25~2000 pg/mL

    灵敏度: 18.75 pg/mL

    货期: 3个工作日+顺丰周期

    在线咨询 比较 加入购物车 说明书

    用途

    该试剂盒用于体外定量检测Human 血清,血浆或其他生物体液中天然及部分重组VEGF-A浓度

    检测原理

    本试剂盒采用双抗体夹心ELISA法。用抗人VEGF-A抗体包被于酶标板上,实验时样品或标准品中的人VEGF-A会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗人VEGF-A抗体和辣根过氧化物酶标记的亲和素。抗人VEGF-A抗体与结合在包被抗体上的人VEGF-A结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,VEGF-A浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中VEGF-A的浓度。

    反应类型 夹心法
    规格 96T
    反应时间 3.5h
    反应性 Human
    检测方法 Colormetric
    检测范围 31.25—2000 pg/mL
    灵敏度 18.75 pg/mL
    样本体积 100μL
    样本类型 血清,血浆或其他生物体液

    特异性

    可检测样本中的人VEGF-A,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average Corrected
    2000 2.491
    2.501
    2.496 2.412
    1000 1.599
    1.631
    1.615 1.531
    500 0.9409999999999999
    0.905
    0.923 0.839
    250 0.426
    0.462
    0.444 0.36
    125 0.276
    0.258
    0.267 0.183
    62.5 0.199
    0.169
    0.184 0.1
    31.25 0.131
    0.141
    0.136 0.052
    0 0.081
    0.08699999999999999
    0.08400000000000001 --

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

    Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    94.10 279.80 737.20 92.40 303.90 745.00
    Standard
    deviation
    5.50 14.30 34.60 4.80 15.50 31.30
    C V (%) 5.84 5.11 4.69 5.19 5.10 4.20

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=5) 92-105 98
    EDTA plasma (n=5) 95-110 101
    Cell culture media (n=5) 88-103 96

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 99-114 97-111 91-105
    Average (%) 105 104 99
    1:4 Range (%) 95-112 80-95 93-106
    Average (%) 103 87 98
    1:8 Range (%) 100-116 86-100 97-110
    Average (%) 106 93 103
    1:16 Range (%) 96-110 82-92 96-107
    Average (%) 104 87 102

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2 支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 2-8℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 2-8℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 2-8℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1. 酶标仪(450nm波长滤光片)
    • 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3. 37℃恒温箱,双蒸水或去离子水
    • 4. 吸水纸

    操作步骤

    1. 对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟

    2. 弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟

    3. 弃掉板内液体,洗板3次

    4. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次

    5. 每孔加入90μL底物溶液,37℃孵育15分钟左右

    6. 每孔加入50μL终止液

    7. 立即在450nm波长下读数,处理数据

    客户发表文献

    1. Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner
      IF: 7.36
      Journal: Cancer letters(2020)
      DOI: 10.1016/j.canlet.2020.08.030
      PMID: 32860852
      引用产品: E-EL-H0111c E-EL-H1863c E-EL-H2428c 
      样本类型: Cell culture supernatant
      物种: Human
    2. A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility
      IF: 6.638
      Journal: Acta Biomaterialia(2019)
      DOI: 10.1016/j.actbio.2019.05.012
      PMID: 31075515
      引用产品: E-EL-H0111c E-EL-0162c E-EL-H1577c 
      样本类型: Cell culture supernatant
      物种: Human
    3. Chrysin Inhibits Melanoma Tumor Metastasis via Interfering with the FOXM1/β-Catenin Signaling
      IF: 4.192
      Journal: Journal of Agricultural and Food Chemistry(2020)
      DOI: 10.1021/acs.jafc.0c03123
      PMID: 32797754
      引用产品: E-EL-0034c E-EL-H0111c 
      样本类型: Cell culture medium
      物种: Human
    4. Activation of the complement system in an osteosarcoma cell line promotes angiogenesis through enhanced production of growth factors.
      IF: 4.259
      Journal: Scientific reports(2018)
      DOI: 10.1038/s41598-018-23851-z
      PMID: 29615744
      引用产品: E-EL-H0111c E-EL-H0071c 
      物种: Human
    5. Melatonin stimulates VEGF expression in human granulosa-lutein cells: A potential mechanism for the pathogenesis of ovarian hyperstimulation syndrome
      IF: 3.871
      Journal: Molecular and Cellular Endocrinology(2020)
      DOI: 10.1016/j.mce.2020.110981
      PMID: 32791190
      引用产品: E-EL-H0111c 
      样本类型: Follicular Fluid
      物种: Human
    6. REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells
      IF: 3.092
      Journal: Biological Research(2020)
      DOI: 10.1186/s40659-020-00307-1
      PMID: 32958054
      引用产品: E-EL-H0111c 
      样本类型: Cell lysate
      物种: Human
    7. Association of rs699947 (-2578 C/A) and rs2010963 (-634 G/C) Single Nucleotide Polymorphisms of the VEGF Gene, VEGF-A and Leptin Serum Level, and Cardiovascular Risk in Patients with Excess Body Mass: A Case-Control Study
      IF: 3.303
      Journal: Journal of clinical medicine(2020)
      DOI: 10.3390/jcm9020469
      PMID: 32046348
      引用产品: E-EL-H0111c E-EL-H0113c 
      样本类型: Serum
      物种: Human
    8. Association of IGF1 and VEGFA polymorphisms with diabetic retinopathy in Pakistani population
      IF: 3.418
      Journal: Acta Diabetologica(2020)
      DOI: 10.1007/s00592-019-01407-5
      PMID: 31473834
      引用产品: E-EL-H0086c E-EL-H0111c 
      样本类型: Serum
      物种: Human
    9. The rhenium(I)-diselenoether Anticancer Drug Targets ROS, TGF-β1, VEGF-A, and IGF-1 in an in Vitro Experimental Model of Triple-Negative Breast Cancers
      IF: 3.502
      Journal: Investigational new drugs(2019)
      DOI: 10.1007/s10637-019-00727-1
      PMID: 30632005
      引用产品: E-EL-H0086c E-EL-H0110c E-EL-H0111c 
      样本类型: Cell culture medium
      物种: Human
    10. Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro
      IF: 3.457
      Journal: Biomedicine & Pharmacotherapy(2019)
      DOI: 10.1016/j.biopha.2019.108706
      PMID: 30844656
      引用产品: E-EL-0162c E-EL-H0023c E-EL-H0088c E-EL-H0102c E-EL-H0109c E-EL-H0111c E-EL-H0149c E-EL-H0483c 
      样本类型: Wound drainage fluid
      物种: Human
    11. The effects of biomimetically conjugated VEGF on osteogenesis and angiogenesis of MSCs (human and rat) and HUVECs co-culture models.
      IF: 3.997
      Journal: Colloids and Surfaces B: Biointerfaces(2018)
      DOI: 10.1016/j.colsurfb.2018.04.060
      PMID: 29730577
      引用产品: E-EL-R2603c E-EL-H0111c 
      样本类型: VEGF solution after immobilization
      物种: Human
    12. The relationship of plasma miR-503 and coronary collateral circulation in patients with coronary artery disease.
      IF: 3.234
      Journal: Life sciences(2018)
      DOI: 10.1016/j.lfs.2018.06.001
      PMID: 29870767
      引用产品: E-EL-H0111c 
      样本类型: Plasma
      物种: Human
    13. Identification Of NCK1 As A Novel Downstream Effector Of STAT3 In Colorectal Cancer Metastasis And Angiogenesis.
      IF: 3.937
      Journal: Cellular Signalling(2017)
      DOI: 10.1016/j.cellsig.2017.04.020
      PMID: 28455144
      引用产品: E-EL-H0111c 
      样本类型: Cell culture supernatant
      物种: Human
    14. NLRC5 Promotes Cell Proliferation Via Regulating The AKT/VEGF-A Signaling Pathway In Hepatocellular Carcinoma.
      IF: 3.582
      Journal: Toxicology(2016)
      DOI: 10.1016/j.tox.2016.06.012
      PMID: 27338800
      引用产品: E-EL-H0111c 
      样本类型: Cell culture supernatant
      物种: Human
    15. Future Prospective and Current Trend of Biomaterials and Growth Factor Used for Maxillofacial Hard and Soft Tissue Reconstruction
      IF: 2.583
      Journal: BioMed research international(2018)
      DOI: 10.1155/2018/3249653
      PMID: 30581851
      引用产品: E-EL-H0111c 
      样本类型: Error information
      物种: Non-living
    16. In vitro photodynamic therapy of endothelial cells using hematoporphyrin monomethyl ether (Hemoporfin): Relevance to treatment of port wine stains
      IF: 2.589
      Journal: Photodiagnosis and Photodynamic Therapy(2019)
      DOI: 10.1016/j.pdpdt.2019.06.003
      PMID: 31185325
      引用产品: E-EL-H0111c 
      样本类型: Cell culture supernatant
      物种: Human
    17. Reduced Plasma miR-146a Is A Predictor Of Poor Coronary Collateral Circulation In Patients With Coronary Artery Disease.
      IF: 2.476
      Journal: BioMed Research International(2016)
      DOI: 10.1155/2016/4285942
      PMID: 28050558
      引用产品: E-EL-H0111c 
      样本类型: Plasma
      物种: Human
    18. The Relationship Of Plasma Decoy Receptor 3 And Coronary Collateral Circulation In Patients With Coronary Artery Disease.
      IF: 2.936
      Journal: Life Sciences(2017)
      DOI: 10.1016/j.lfs.2017.09.025
      PMID: 28942284
      引用产品: E-EL-H5587c E-EL-H0111c 
      样本类型: Plasma
      物种: Human
    19. Thicker carotid intima-media thickness and increased plasma VEGF levels suffered by post-acute thrombotic stroke patients
      IF: 1.927
      Journal: International Journal of General Medicine(2016)
      DOI: 10.2147/IJGM.S114577
      PMID: 28008280
      引用产品: E-EL-H0111c 
      样本类型: Plasma
      物种: Human
    20. Evaluation of the growth factors VEGF-a and VEGF-B in the vitreous and serum of patients with macular and retinal vascular diseases.
      IF: 1.8
      Journal: Growth Factors(2018)
      DOI: 10.1080/08977194.2018.1477140
      PMID: 29969324
      引用产品: E-EL-H2164c E-EL-H0111c 
      样本类型: Serum
      物种: Human
    21. The Antiangiogenesis Effect Of Pirfenidone In Wound Healing In Vitro.
      IF: 1.679
      Journal: Journal of Ocular Pharmacology And Therapeutics(2017)
      DOI: 10.1089/jop.2017.0007
      PMID: 28933986
      引用产品: E-EL-H0111c 
      样本类型: Cell culture supernatant
      物种: Human
    22. Evaluation of Cytokines Response in Plasmodium falciparum Infection among Adolescents in Port Harcourt, Nigeria
      Journal: JMSCR(2018)
      DOI: 10.18535/jmscr/v6i9.54
      PMID: 99999999
      引用产品: E-EL-H0102c E-EL-H0109c E-EL-H0110c E-EL-H0111c 
      样本类型: Plasma
      物种: Human
    23. Level of VEGF-A and interleukin 6 in lacrimal fluid of patients with diabetic retinopathy
      Journal: International Journal of Biological and Chemical Sciences(2020)
      DOI: 10.4314/ijbcs.v14i3.2
      PMID: 99999999
      引用产品: E-EL-H0102c E-EL-H0111c 
      样本类型: Tears
      物种: Human
    24. Relationship between Angiogenic and Inflammatory Biomarkers and Diabetic Retinopathy
      Journal: Open Journal of Ophthalmology(2020)
      DOI: 10.4236/ojoph.2020.101006
      PMID: 99999999
      引用产品: E-EL-H0102c E-EL-H0111c 
      样本类型: Serum
      物种: Human
    25. Growth factors profile in conditioned medium human adipose tissue-derived mesenchymal stem cells (CM-hATMSCs)
      Journal: Clinical Nutrition Experimental(2019)
      DOI: 10.1016/j.yclnex.2019.01.002
      PMID: 99999999
      引用产品: E-EL-H0059c E-EL-H0110c E-EL-H0111c E-EL-H0483c E-EL-H1587c E-EL-H5587c 
      样本类型: Cell culture medium
      物种: Human
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