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Mitochondrial Membrane Potential Assay Kit (with JC-1)

  • 中文名称:线粒体膜电位检测试剂盒(JC-1)

  • 货号:E-CK-A301

订购 E-CK-A301

规格:
  • 20Assays
  • 50Assays
  • 100Assays
价格: ¥260
数量:

检测原理

线粒体膜电位下降是细胞凋亡早期的标志性事件,发生在细胞核凋亡特征(染色质浓缩、DNA断裂)出现之前,一旦线粒体膜电位崩溃,细胞凋亡便不可逆转。 JC-1是一种广泛用于检测线粒体膜电位∆Ψm的理想荧光探针,可以检测细胞、组织或纯化的线粒体膜电位。JC-1有单体和多聚体两种存在形式,两者发射光谱不同。在正常细胞内,线粒体膜电位较高,JC-1聚集在线粒体的基质中,形成聚合物,可产生红色荧光;凋亡早期,线粒体膜电位降低,JC-1不能聚集在线粒体基质中,此时JC-1为单体,可产生绿色荧光。 通过JC-1从红色荧光到绿色荧光的转变可检测到细胞膜电位的下降,可将JC-1荧光颜色的转变作为细胞凋亡早期的检测指标。常用红绿荧光的相对比例来衡量线粒体去极化的比例。 JC-1单体的最大激发波长为514 nm,最大发射波长为529 nm;JC-1聚合物的最大激发波长为585 nm,最大发射波长为590 nm。实际观察时,常规设置红色荧光和绿色荧光即可。

基本信息

应用类型 细胞凋亡-JC-1
检测方法 荧光
样本类型 细胞
检测时间 40min
检测仪器 流式细胞仪,荧光显微镜,激光共聚焦显微镜
荧光 JC-1
Ex/Em (nm) 514/529, 585/590
Channel set FITC, PE
Filter set FITC, TRITC
自备试剂 超纯水
保存温度 2~8°C/-20°C, shading light. Refer to the Manual.
运输条件 冰袋
保质期 12 months

文献

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