Mouse Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)
Price:
- 宿主: Mouse
- 应用性: WB;ELISA
Source | Mouse |
Immunogen | Rabbit IgG |
Application | WB, ELISA |
Recommended Dilution | WB 1:2000-5000(Enhanced chemiluminescent detection) WB 1:300-1500(DAB detection) Direct ELISA 1:3000-5000(TMB detection) |
Concentration | 0.5 mg/mL |
Clonality | Polyclonal |
Purification | Affinity purification |
Conjugation | HRP |
Storage Instructions | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Storage Buffer | PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4 |
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IF: 3.848Journal:MICROBIAL PATHOGENESIS
Q1:该如何选择二抗?
二抗根据一抗的来源和客户的实验需求来选择。 先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗:如一抗是兔来源的IgG,那就选抗兔IgG的二抗,例如Goat Anti-Rabbit IgG;如一抗是小鼠来源的IgM,那就选抗小鼠IgM的二抗,例如Goat Anti-Mouse IgM。 标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。 ELISA常用Biotin标记的二抗,后接一个链霉亲和素标记的HRP起到进一步信号放大作用,用TMB底物显色; WB主要选择HRP标记的二抗,用ECL底物发光液显色; IHC一般也是选择HRP标记的二抗,推荐使用免疫组化试剂盒(E-IR-R217或最新的E-IR-R220/221),一般用DAB底物液显色; IF可按实验需要选择不同荧光素标记的二抗,如FITC、Cy3、PE等均可(尽量避开细胞的自发荧光),通过荧光显微镜检测荧光。