Mouse ICAM-1/CD54(intercellular adhesion molecule 1) ELISA Kit
中文名称:小鼠细胞间粘附分子1(ICAM-1/CD54)酶联免疫吸附测定试剂盒
别称:
ICAM1、BB2、CD54、P3.58、Intercellular adhesion molecule 1
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 1.37-1000 ng/mL
- 灵敏度: 0.82 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠ICAM-1/CD54抗体包被于酶标板上,实验时样品(或标准品)中的小鼠ICAM-1/CD54会与包被抗体结合。后依次加入生物素化的抗小鼠ICAM-1/CD54抗体和辣根过氧化物酶标记的亲和素,抗小鼠ICAM-1/CD54抗体与结合在包被抗体上的小鼠ICAM-1/CD54结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,ICAM-1/CD54浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中ICAM-1/CD54的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 1.37-1000 ng/mL |
灵敏度 | 0.82 ng/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠ICAM-1/CD54,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy
IF: 8.063Journal:Theranostics -
Retinol-Binding Protein 4 Accelerates Metastatic Spread and Increases Impairment of Blood Flow in Mouse Mammary Gland Tumors
IF: 6.126Journal:Cancers -
Oral administration of 3,3′-diselenodipropionic acid prevents thoracic radiation induced pneumonitis in mice by suppressing NF-kB/IL-17/G-CSF/neutrophil axis
IF: 5.657Journal:FREE RADICAL BIOLOGY AND MEDICINE -
A novel mechanism of diabetic vascular endothelial dysfunction: Hypoadiponectinemia-induced NLRP3 inflammasome activation
IF: 5.476Journal:BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE -
Combination Therapy with DETA/NO and Clopidogrel Inhibits Metastasis in Murine Mammary Gland Cancer Models via Improved Vasoprotection
IF: 4.556 -
Endothelial progenitor cell–derived exosomes ameliorate endothelial dysfunction in a mouse model of diabetes
IF: 4.545Journal:BIOMEDICINE & PHARMACOTHERAPY -
Blockade of intercellular adhesion molecule-1 prevents angiotensin II-induced hypertension and vascular dysfunction
IF: 3.684Journal:LABORATORY INVESTIGATION -
Diet-Derived Advanced Glycation End Products (dAGEs) Induce Proinflammatory Cytokine Expression in Cardiac and Renal Tissues of Experimental Mice: Protective Effect of Curcumin
IF: 3.239 -
Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet
IF: 2.806Journal:PLoS One -
Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice
IF: 1.917Journal:BIOMEDICAL AND ENVIRONMENTAL SCIENCES