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Mouse IL-1β(Interleukin 1 Beta) ELISA Kit

  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
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  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
    产品货号:E-EL-M0037c

    中文名:小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒

    别称:IL1B, IL1-BETA, IL1F2, catabolin

    规格:
    • 96T
    • 48T
    数量:
    - +
    价格: ¥2400

    反应性: 小鼠

    检测范围: 7.81~500 pg/mL

    灵敏度: 4.69 pg/mL

    货期: 3个工作日+顺丰周期

    加入购物车 比较 咨询 说明书

    用途

    该试剂盒用于体外定量检测Mouse 血清,血浆或其他生物体液中天然及部分重组IL-1β浓度

    检测原理

    本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上,实验时样品或标准品中的小鼠IL-1β会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素。抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IL-1β浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-1β的浓度。

    反应类型 夹心法
    规格 96T
    反应时间 3.5h
    反应性 Mouse
    检测方法 Colormetric
    检测范围 7.81—500 pg/mL
    灵敏度 4.69 pg/mL
    样本体积 100μL
    样本类型 血清,血浆或其他生物体液

    特异性

    可检测样本中的小鼠IL-1β,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average Corrected
    500 2.468
    2.484
    2.476 2.417
    250 1.547
    1.603
    1.575 1.516
    125 0.953
    0.951
    0.952 0.893
    62.5 0.47
    0.492
    0.481 0.422
    31.25 0.246
    0.236
    0.241 0.182
    15.63 0.167
    0.155
    0.161 0.102
    7.81 0.107
    0.115
    0.111 0.052
    0 0.056
    0.062
    0.059 --

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

    Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    26.40 59.30 201.20 27.30 57.80 199.40
    Standard
    deviation
    1.70 3.20 10.10 1.80 2.40 8.80
    C V (%) 6.44 5.40 5.02 6.59 4.15 4.41

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=5) 88-103 96
    EDTA plasma (n=5) 91-104 97
    Cell culture media (n=5) 91-107 98

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 89-101 96-107 85-99
    Average (%) 95 101 92
    1:4 Range (%) 98-112 86-98 92-109
    Average (%) 105 93 100
    1:8 Range (%) 98-116 86-99 93-105
    Average (%) 106 91 100
    1:16 Range (%) 98-113 85-97 91-104
    Average (%) 107 91 97

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2 支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 2-8℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 2-8℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 2-8℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1. 酶标仪(450nm波长滤光片)
    • 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3. 37℃恒温箱,双蒸水或去离子水
    • 4. 吸水纸

    操作步骤

    1. 对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟

    2. 弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟

    3. 弃掉板内液体,洗板3次

    4. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次

    5. 每孔加入90μL底物溶液,37℃孵育15分钟左右

    6. 每孔加入50μL终止液

    7. 立即在450nm波长下读数,处理数据

    客户发表文献

    1. CD47-targeted bismuth selenide nanoparticles actualize improved photothermal therapy by increasing macrophage phagocytosis of cancer cells
      IF: 3.973
      Journal: Colloids and Surfaces B: Biointerfaces(2019)
      DOI: 10.1016/j.colsurfb.2019.110546
      PMID: 31606701
      引用产品: E-EL-M0037c 
      样本类型: Plasma
      物种: Mouse
    2. Design, Synthesis and Investigation of the Potential Anti-inflammatory Activity of 7-O-Amide Hesperetin Derivatives
      IF: 3.06
      Journal: Molecules(2019)
      DOI: 10.3390/molecules24203663
      PMID: 31614601
      引用产品: E-EL-M0037c E-EL-M0044c E-EL-M0049c 
      样本类型: Cell culture supernatant
      物种: Mouse
    3. Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.
      IF: 3.457
      Journal: Biomedicine & Pharmacotherapy(2018)
      DOI: 10.1016/j.biopha.2018.07.081
      PMID: 30119204
      引用产品: E-EL-M0037c 
      样本类型: Serum
      物种: Rat
    4. Propofol specifically suppresses IL-1β secretion but increases bacterial survival in Staphylococcus aureus-infected RAW264. 7 cells.
      IF: 2.561
      Journal: Molecular and Cellular Biochemistry(2018)
      DOI: 10.1007/s11010-018-3348-2
      PMID: 29667111
      引用产品: E-EL-M0037c 
      样本类型: cell
      物种: Mouse
    5. Jinxin oral liquid inhibits human respiratory syncytial virus-induced excessive inflammation associated with blockade of the NLRP3/ASC/Caspase-1 pathway.
      IF: 3.457
      Journal: Biomedicine & pharmacotherapy(2018)
      DOI: 10.1016/j.biopha.2018.04.174
      PMID: 29864921
      引用产品: E-EL-M2642c E-EL-M0037c E-EL-M0730c 
      样本类型: Serum,Tissue homogenate
      物种: Mouse
    6. The protective effect of formononetin on cognitive impairment in streptozotocin (STZ)-induced diabetic mice.
      IF: 3.457
      Journal: Biomedicine & Pharmacotherapy(2018)
      DOI: 10.1016/j.biopha.2018.07.063
      PMID: 30119194
      引用产品: E-EL-M0049c E-EL-M0037c E-EL-M0044c 
      样本类型: Serum,Tissue homogenate
      物种: Mouse
    7. The Association Between Inflammation, The Microbiome and Urethane-induced Pulmonary Adenocarcinoma.
      IF: 1.39
      Journal: Oncology Letters(2018)
      DOI: 10.3892/ol.2018.8167
      PMID: 29725395
      引用产品: E-EL-M0838c E-EL-M0049c E-EL-M0037c E-EL-M0044c 
      物种: Mouse
    8. Oral supplementation with ursolic acid ameliorates sepsis-induced acute kidney injury in a mouse model by inhibiting oxidative stress and inflammatory responses.
      IF: 1.692
      Journal: Molecular Medicine Reports(2018)
      DOI: 10.3892/mmr.2018.8767
      PMID: 29568928
      引用产品: E-EL-M0037c E-EL-M0044c 
      物种: Mouse
    9. Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines.
      IF: 9.326
      Journal: Bone research(2018)
      DOI: 10.1038/s41413-018-0012-0
      PMID: 29619269
      引用产品: E-EL-M0036c E-EL-M0037c E-EL-M0044c E-EL-M0049c E-EL-M0857c E-EL-M1382c 
      物种: Mouse
    10. Quercetin Inhibits LPS-Induced Inflammation and ox-LDL-Induced Lipid Deposition.
      IF: 3.831
      Journal: Front Pharmacol(2017)
      DOI: 10.3389/fphar.2017.00040
      PMID: 28217098
      引用产品: E-EL-M0954c E-EL-M0037c E-EL-M0959c 
      样本类型: Cell
      物种: Mouse
    11. Blocking The CD38/cADPR Pathway Plays A Double-edged Role In LPS Stimulated Microglia.
      IF: 3.277
      Journal: Neuroscience(2017)
      DOI: 10.1016/j.neuroscience.2017.08.010
      PMID: 28807785
      引用产品: E-EL-M0049c E-EL-M0044c E-EL-M0037c 
      样本类型: Cell culture supernatant
      物种: Mouse
    12. Wu-tou Decoction Attenuates Neuropathic Pain Via Suppressing Spinal Astrocytic IL-1R1/TRAF6/JNK Signaling.
      IF: 5.168
      Journal: Oncotarget(2017)
      DOI: 10.18632/oncotarget.21638
      PMID: 29190962
      引用产品: E-EL-M0049c E-EL-M0037c E-EL-M0018c 
      样本类型: Tissue homogenate
      物种: Mouse
    13. Molecular Mechanisms Of the Analgesic Action Of Wu-tou Decoction On Neuropathic Pain In Mice Revealed Using Microarray And Network Analysis.
      IF: 3.223
      Journal: Acta Pharmacologica Sinica(2017)
      DOI: 10.1038/aps.2017.110
      PMID: 28816231
      引用产品: E-EL-M0037c E-EL-M0049c E-EL-M0018c 
      样本类型: Tissue homogenate
      物种: Mouse
    14. Osthole Prevents Acetaminophen-Induced Liver Injury In Mice.
      IF: 3.223
      Journal: Acta Pharmacologica Sinica(2017)
      DOI: 10.1038/aps.2017.129
      PMID: 29022574
      引用产品: E-EL-M2567c E-EL-M0037c E-EL-M0044c E-EL-0083c 
      样本类型: Tissue homogenate
      物种: Mouse
    15. Effects Of Sepsis On The Metabolism Of Sphingomyelin And Cholesterol In Mice With Liver Dysfunction.
      IF: 1.261
      Journal: Experimental and Therapeutic Medicine(2017)
      DOI: 10.3892/etm.2017.5226
      PMID: 29285103
      引用产品: E-EL-M0037c E-EL-M0049c 
      样本类型: Plasma
      物种: Mouse
    16. Repression Of Toll-Like Receptor-4 By microRNA-149-3p Is Associated With Smoking-Related COPD.
      IF: 3.157
      Journal: International Journal of Chronic Obstructive Pulmonary Disease(2017)
      DOI: 10.2147/COPD.S128031
      PMID: 28260877
      引用产品: E-EL-M0037c E-EL-M0049c 
      样本类型: Cell culture supernatant
      物种: Mouse
    17. Glycyrrhiza Uralensis Water Extract Enhances Dendritic Cell Maturation And Antitumor Efficacy Of HPV Dendritic Cell-Based Vaccine.
      IF: 4.259
      Journal: Scientific Reports(2017)
      DOI: 10.1038/srep43796
      PMID: 28272545
      引用产品: E-EL-M0037c E-EL-M0044c E-EL-M0726c E-EL-M0049c 
      样本类型: Cell culture supernatant
      物种: Mouse
    18. The TIR/BB-Loop Mimetic AS-1 Prevents Non-Alcoholic Steatohepatitis And Hepatic Insulin Resistance By Inhibiting NLRP3-ASC Inflammasome Activation.
      IF: 5.491
      Journal: British Journal of Pharmacology(2017)
      DOI: 10.1111/bph.13786
      PMID: 28306139
      引用产品: E-EL-M0037c 
      样本类型: Cell culture supernatant
      物种: Mouse
    19. Celastrol-Induced Nur77 Interaction With TRAF2 Alleviates Inflammation By Promoting Mitochondrial Ubiquitination And Autophagy.
      IF: 14.714
      Journal: Molecular Cell(2017)
      DOI: 10.1016/j.molcel.2017.03.008
      PMID: 28388439
      引用产品: E-EL-M0037c E-EL-M0044c 
      样本类型: Serum
      物种: mouse
    20. A Novel Mechanism Of Diabetic Vascular Endothelial Dysfunction: Hypoadiponectinemia-Induced NLRP3 Inflammasome Activation.
      IF: 5.476
      Journal: Biochimica Et Biophysica Acta-Molecular Basis of Disease(2017)
      DOI: 10.1016/j.bbadis.2017.02.012
      PMID: 28216285
      引用产品: E-EL-M0037c E-EL-M0730c E-EL-M0077c E-EL-M0647c 
      样本类型: Serum,Cell culture supernatant
      物种: Mouse
    21. Capparis Spinosa Fruit Ethanol Extracts Exert Different Effects On The Maturation Of Dendritic Cells.
      IF: 2.861
      Journal: Molecules(2017)
      DOI: 10.3390/molecules22010097
      PMID: 28067853
      引用产品: E-EL-M2451c E-EL-M0046c E-EL-M0044c E-EL-M0037c E-EL-M0049c 
      样本类型: Cell culture supernatant
      物种: Mouse
    22. Liraglutide Attenuates Lipopolysaccharide-Induced Acute Lung Injury In Mice.
      IF: 2.896
      Journal: European Journal of Pharmacology(2016)
      DOI: 10.1016/j.ejphar.2016.10.016
      PMID: 27756605
      引用产品: E-EL-M0037c E-EL-M0730c 
      样本类型: Bronchoalveolar Lavage Fluid
      物种: Mouse
    23. The TIR/BB-Loop Mimetic AS-1 Attenuates Mechanical Stress-Induced Cardiac Fibroblast Activation And Paracrine Secretion Via Modulation Of Large Tumor Suppressor Kinase 1.
      IF: 5.476
      Journal: Biochimica Et Biophysica Acta-Molecular Basis of Disease(2016)
      DOI: 10.1016/j.bbadis.2016.03.002
      PMID: 26964796
      引用产品: E-EL-M0049c E-EL-M0037c 
      样本类型: Cell culture supernatant
      物种: Mouse
    24. The Effect Of Maternal Obesity On The Expression And Functionality Of Placental P-Glycoprotein: Implications In The Individualized Transplacental Digoxin Treatment For Fetal Heart Failure.
      IF: 2.759
      Journal: Placenta(2015)
      DOI: 10.1016/j.placenta.2015.08.007
      PMID: 26311557
      引用产品: E-EL-M0037c E-EL-M0049c 
      样本类型: Plasma
      物种: Mouse
    25. PGC-1α Silencing Compounds The Perturbation Of Mitochondrial Function Caused By Mutant SOD1 In Skeletal Muscle Of ALS Mouse Model.
      IF: 4.504
      Journal: Frontiers in Aging Neuroscience(2015)
      DOI: 10.3389/fnagi.2015.00204
      PMID: 26539112
      引用产品: E-EL-M0049c E-EL-M0037c E-EL-M0044c E-EL-M0007c 
      样本类型: Serum
      物种: Mouse
    26. A Comparative Study Of The Effects Upon LPS Induced Macrophage RAW264.7 Inflammation In Vitro Of The Lipids Of Hippocampus Trimaculatus Leach.
      IF: 1.076
      Journal: Journal of Oleo Science(2015)
      DOI: 10.5650/jos.ess15190
      PMID: 26582156
      引用产品: E-EL-M0049c E-EL-M0037c E-EL-M0044c 
      样本类型: Cell culture supernatant
      物种: Mouse
    27. Ghrelin Ameliorates Intestinal Barrier Dysfunction In Experimental Colitis By Inhibiting The Activation Of Nuclear Factor-Kappa B.
      IF: 2.466
      Journal: Biochemical & Biophysical Research Communications(2015)
      DOI: 10.1016/j.bbrc.2015.01.083
      PMID: 25634696
      引用产品: E-EL-M0049c E-EL-M0048c E-EL-M0037c E-EL-M0044c E-EL-M0046c E-EL-M0047c 
      样本类型: Tissue homogenate
      物种: Mouse
    28. Gentiopicroside abrogates lipopolysaccharide-induced depressive-like behavior in mice through tryptophan-degrading pathway.
      IF: 2.441
      Journal: Metabolic Brain Disease
      DOI: 10.1007/s11011-018-0246-y
      PMID: 29948656
      引用产品: E-EL-M0049c E-EL-M0037c 
      样本类型: Plasma
      物种: Mouse
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