本公司产品仅供体外研究使用,不用于临床诊断

Mouse IL-2(Interleukin 2) ELISA Kit

新产品推荐: E-MSEL-M0036
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
<
>
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
    产品货号:E-EL-M0042c

    中文名:小鼠白介素2(IL-2)酶联免疫吸附测定试剂盒

    别称:IL2, TCGF, Lymphokine

    规格:
    • 96T
    • 48T
    • 96T*5
    数量:
    - +
    价格: ¥2400

    反应性: 小鼠

    检测范围: 15.63~1000 pg/mL

    灵敏度: 9.38 pg/mL

    用途

    该试剂盒用于体外定量检测Mouse 血清,血浆或其他生物体液中IL-2浓度

    检测原理

    本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-2抗体包被于酶标板上,实验时样品或标准品中的小鼠IL-2会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗小鼠IL-2抗体和辣根过氧化物酶标记的亲和素。抗小鼠IL-2抗体与结合在包被抗体上的小鼠IL-2结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IL-2浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-2的浓度。

    反应类型 夹心法
    规格 96T
    反应时间 3.5h
    反应性 Mouse
    检测方法 Colormetric
    检测范围 15.63—1000 pg/mL
    灵敏度 9.38 pg/mL
    样本体积 100μL
    样本类型 血清,血浆或其他生物体液

    特异性

    可检测样本中的小鼠IL-2,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average Corrected
    1000 2.453
    2.465
    2.459 2.381
    500 1.596
    1.622
    1.609 1.531
    250 0.964
    0.964
    0.964 0.886
    125 0.475
    0.511
    0.493 0.415
    62.5 0.272
    0.26
    0.266 0.188
    31.25 0.18
    0.18
    0.18 0.102
    15.63 0.126
    0.134
    0.13 0.052
    0 0.077
    0.079
    0.078 --

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

    Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    49.00 87.40 385.60 52.40 82.20 365.00
    Standard
    deviation
    3.30 3.80 20.40 3.10 4.80 15.70
    C V (%) 6.73 4.35 5.29 5.92 5.84 4.30

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=8) 87-101 93
    EDTA plasma (n=8) 88-103 94
    Cell culture media (n=8) 89-102 96

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 88-101 93-107 93-107
    Average (%) 95 100 99
    1:4 Range (%) 95-112 81-94 92-106
    Average (%) 103 86 98
    1:8 Range (%) 97-111 84-97 95-110
    Average (%) 105 89 101
    1:16 Range (%) 100-117 82-92 91-107
    Average (%) 108 87 98

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2 支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 2-8℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 2-8℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 2-8℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1. 酶标仪(450nm波长滤光片)
    • 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3. 37℃恒温箱,双蒸水或去离子水
    • 4. 吸水纸

    操作步骤

    1. 对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟

    2. 弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟

    3. 弃掉板内液体,洗板3次

    4. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次

    5. 每孔加入90μL底物溶液,37℃孵育15分钟左右

    6. 每孔加入50μL终止液

    7. 立即在450nm波长下读数,处理数据

    客户发表文献

    1. Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy
      IF: 16.304
      Journal: Science Translational Medicine(2020)
      DOI: 10.1126/scitranslmed.aaz6667
      PMID: 33239389
      样本类型: Cell culture supernatant
      物种: Mouse
    2. Highly tailorable gellan gum nanoparticles as a platform for the development of T cell activator systems
      IF: 15.863
      Journal: Biomaterials Research(2022)
      DOI: 10.1186/s40824-022-00297-z
      PMID: 36180901
      样本类型: splenocytes
      物种: Mouse
    3. Glucocorticoid-induced loss of beneficial gut bacterial extracellular vesicles is associated with the pathogenesis of osteonecrosis
      IF: 14.957
      Journal: Science Advances(2022)
      DOI: 10.1126/sciadv.abg8335
      样本类型: serum
      物种: Mouse
    4. Treg-targeted efficient-inducible platform for collagen-induced arthritis treatment
      IF: 10.761
      Journal: Materials Today Bio(2023)
      DOI: 10.1016/j.mtbio.2023.100557
      样本类型: Lymphocytes
      物种: Mouse
    5. Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation
      IF: 9.381
      Journal: CARBOHYDRATE POLYMERS(2021)
      DOI: 10.1016/j.carbpol.2021.118211
      PMID: 34127215
      样本类型: Tissue Slice
      物种: Mouse
    6. Structural characterization of a soluble polysaccharide SSPS1 from soy whey and its immunoregulatory activity in macrophages
      IF: 8.025
      Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES(2022)
      DOI: 10.1016/j.ijbiomac.2022.07.043
      PMID: 35835298
      样本类型: Raw 264.7 macrophages
      物种: Mouse
    7. Aronia melanocarpa Extract Fermented by Lactobacillus plantarum EJ2014 Modulates Immune Response in Mice
      IF: 6.313
      Journal: Antioxidants(2021)
      DOI: 10.3390/antiox10081276
      PMID: 34439524
      样本类型: plasma
      物种: Mouse
    8. Immunomodulatory effects and mechanisms of Tiepishihu Xiyangshen granules on cyclophosphamide induced immuno-suppression via TLR4/MAPKs and PI3K/AKT/FOXO3a signal pathways
      IF: 5.195
      Journal: JOURNAL OF ETHNOPHARMACOLOGY(2023)
      DOI: 10.1016/j.jep.2023.116192
      样本类型: serum
      物种: Mouse
    9. A different approach to immunochemotherapy for colon Cancer: Development of nanoplexes of cyclodextrins and Interleukin-2 loaded with 5-FU
      IF: 5.875
      Journal: INTERNATIONAL JOURNAL OF PHARMACEUTICS(2022)
      DOI: 10.1016/j.ijpharm.2022.121940
      PMID: 35724824
      样本类型: CD nanoplexes
    10. Erianin inhibits human lung cancer cell growth via PI3K/Akt/mTOR pathway in vitro and in vivo
      IF: 5.882
      Journal: PHYTOTHERAPY RESEARCH(2021)
      DOI: 10.1002/ptr.7154
      PMID: 34236105
      样本类型: LLC cell-Xenograft
      物种: Mouse
    11. Antitumor Immunotherapy of Sialic Acid and/or GM1 Modified Coenzyme Q10 Submicron Emulsion
      IF: 4.026
      Journal: AAPS PHARMSCITECH(2022)
      DOI: 10.1208/s12249-022-02426-2
      PMID: 36253573
      样本类型: serum
      物种: Mouse
    12. Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis
      IF: 4.074
      Journal: CARBOHYDRATE POLYMERS(2015)
      DOI: 10.1016/j.carbpol.2015.12.050
      样本类型: Cell culture supernatant
      物种: Mouse
    13. Immunization With a Secreted Esterase Protects Mice Against Multiple Serotypes (M1, M3, and M28) of Group A Streptococcus
      IF: 4.236
      Journal: Frontiers in Microbiology(2020)
      DOI: 10.3389/fmicb.2020.00565
      样本类型: skin
      物种: Mouse
    14. Rheb1 deletion in myeloid cells aggravates OVA-induced allergic inflammation in mice
      IF: 4.259
      Journal: Scientific Reports(2017)
      DOI: 10.1038/srep42655
      样本类型: Bronchoalveolar Lavage Fluid,Serum
      物种: Mouse
    15. A decrease in cluster of differentiation 2 expression on natural killer cells is associated with polycystic ovary syndrome but not influenced by metformin in a mouse model
      IF: 4.285
      Journal: BIOLOGY OF REPRODUCTION(2022)
      DOI: 10.1093/biolre/ioac004
      样本类型: serum
      物种: Mouse
    16. Danggui Buxue Decoction enhances the anticancer activity of gemcitabine and alleviates gemcitabine-induced myelosuppression
      IF: 4.36
      Journal: JOURNAL OF ETHNOPHARMACOLOGY(2021)
      DOI: 10.1016/j.jep.2021.113965
      PMID: 33639205
      样本类型: Serum,Tissue(spleen)
      物种: Mouse
    17. Attenuation of virulence in multiple serotypes (M1, M3, and M28) of Group A Streptococcus after the loss of secreted esterase
      IF: 4.399
      Journal: JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION(2021)
      DOI: 10.1016/j.jmii.2021.09.008
      PMID: 34674958
      样本类型: skin lesion
      物种: Mouse
    18. The immunomodulatory effects of ginsenoside derivative Rh2-O on splenic lymphocytes in H22 tumor-bearing mice is partially mediated by TLR4
      IF: 4.932
      Journal: INTERNATIONAL IMMUNOPHARMACOLOGY(2021)
      DOI: 10.1016/j.intimp.2021.108316
      PMID: 34768129
      样本类型: splenic lymphocytes
      物种: Mouse
    19. Antitumor and immunomodulatory effects of ginsenoside Rh2 and its octyl ester derivative in H22 tumor-bearing mice
      IF: 3.144
      Journal: Journal of Functional Foods(2017)
      DOI: 10.1016/j.jff.2017.03.013
      样本类型: Serum
      物种: Mouse
    20. Structural characterization of two endopolysaccharides from Phellinus sp. and their immunologic effects by intragastric administration in a healthy mammalian model
      IF: 3.289
      Journal: Food & Function(2018)
      DOI: 10.1039/C7FO01161J
      样本类型: serum
      物种: Mouse
    21. Molecular and functional characterization of HtrA protein in Actinobacillus pleuropneumoniae
      IF: 3.293
      Journal: VETERINARY MICROBIOLOGY(2021)
      DOI: 10.1016/j.vetmic.2021.109058
      PMID: 33862332
      样本类型: Serum
      物种: Mouse
    22. Fusion of thymosin alpha 1 with mutant IgG1 CH3 prolongs half-life and enhances antitumor effects in vivo
      IF: 3.361
      Journal: INTERNATIONAL IMMUNOPHARMACOLOGY(2019)
      DOI: 10.1016/j.intimp.2019.05.047
      PMID: 31220695
      样本类型: Serum
      物种: Mouse
    23. Immunogenicity of the recombinant HxuCBA proteins encoded by hxuCBA gene cluster of Haemophilus parasuis in mice
      IF: 3.688
      Journal: GENE(2016)
      DOI: 10.1016/j.gene.2016.07.001
      样本类型: Serum
      物种: Mouse
    24. In vivo and in vitro efficacy of crocin against Echinococcus multilocularis
      IF: 3.876
      Journal: Parasites & Vectors(2021)
      DOI: 10.1186/s13071-021-04866-4
      PMID: 34256821
      样本类型: serum
      物种: Mouse
    25. In vitro and in vivo efficacy of thiacloprid against Echinococcus multilocularis
      IF: 3.876
      Journal: Parasites & Vectors(2021)
      DOI: 10.1186/s13071-021-04952-7
      PMID: 34488852
      样本类型: serum
      物种: Mouse
    26. Plasmodium knowlesi Duffy binding protein alpha region II (PkDBPαII) in clinical isolates from Peninsular Malaysia and Malaysian Borneo exhibit different immune responses in animal models
      IF: 2.383
      Journal: PARASITOLOGY RESEARCH(2022)
      DOI: 10.1007/s00436-022-07665-7
      PMID: 36152079
      样本类型: Spleen cell
      物种: Mouse
    27. Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota
      IF: 2.581
      Journal: MICROBIAL PATHOGENESIS(2019)
      DOI: 10.1016/j.micpath.2019.103557
      样本类型: serum,small intestinal tissues
      物种: Mouse
    28. A trivalent Apx-fusion protein delivered by E. coli outer membrane vesicles induce protection against Actinobacillus pleuropneumoniae of serotype 1 and 7 challenge in a murine model
      IF: 2.766
      Journal: PLoS One(2018)
      DOI: 10.1371/journal.pone.0191286
      样本类型: serum
      物种: Mouse
    29. Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection
      IF: 2.914
      Journal: MICROBIAL PATHOGENESIS(2020)
      DOI: 10.1016/j.micpath.2020.104441
      PMID: 32781100
      样本类型: Serum,Cell culture supernatant
      物种: Mouse
    30. Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF−κB activation
      IF: 2.914
      Journal: MICROBIAL PATHOGENESIS(2020)
      DOI: 10.1016/j.micpath.2020.104054
      样本类型: Serum
      物种: Mouse
    31. BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice
      IF: 2.952
      Journal: Molecular Medicine Reports(2019)
      DOI: 10.3892/mmr.2019.10780
      样本类型: BMDCs
      物种: Mouse
    32. Effect of xanthohumol on Th1/Th2 balance in a breast cancer mouse model
      IF: 2.976
      Journal: ONCOLOGY REPORTS(2018)
      DOI: 10.3892/or.2017.6094
      样本类型: serum
      物种: Mouse
    33. Sulfated modification promotes the immunomodulatory bioactivities of Lyciumbarbarum polysaccharides in vitro
      IF: 1.277
      Journal: International Journal of Clinical and Experimental Medicine(2015)
      PMID: 26884954
      样本类型: lymphocyte
      物种: Mouse
    34. Evaluation of Allicin Against Alveolar Echinococcosis In Vitro and in a Mouse Model
      IF: 1.44
      Journal: ACTA PARASITOLOGICA(2021)
      DOI: 10.1007/s11686-021-00434-z
      PMID: 34143400
      样本类型: serum
      物种: Mouse
    35. Amelioration effects of Kaempferol on immune response following chronic intermittent cold-stress
      IF: 1.751
      Journal: RESEARCH IN VETERINARY SCIENCE(2019)
      DOI: 10.1016/j.rvsc.2019.08.012
      PMID: 31412308
      样本类型: Serum,Tissue homogenate
      物种: Mouse
    36. Colonisation of mice and pigs by a chimeric porcine circovirus 1–2 prototype vaccine strain and a PCV2 isolate originating in China and their induction of cytokines
      IF: 1.786
      Journal: JOURNAL OF VIROLOGICAL METHODS(2020)
      DOI: 10.1016/j.jviromet.2020.113905
      样本类型: Cell culture supernatant
      物种: Mouse
    展开收起
    写评论得优惠
    • 全部 (0)
    • 评论 (0)
    • 问答 (0)
    ... 展开 收起

    ELISA样本处理试剂

    操作说明下载

    我的购物车

    产品名称 数量 价格
    结算

    总计:

    诚邀您参与Elabscience生化试剂盒性能验证计划还有积分奖励等你来赢!

    浏览历史


      买了该产品的人还买了