Mouse LH(Luteinizing Hormone) ELISA Kit
中文名称:小鼠促黄体生成激素(LH)酶联免疫吸附测定试剂盒
别称:
Lutropin、Lutrophin
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 0.31-20 ng/mL
- 灵敏度: 0.19 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠LH抗体包被于酶标板上,实验时样品(或标准品)中的小鼠LH会与包被抗体结合。后依次加入生物素化的抗小鼠LH抗体和辣根过氧化物酶标记的亲和素,抗小鼠LH抗体与结合在包被抗体上的小鼠LH结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,LH浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中LH的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 0.31-20 ng/mL |
灵敏度 | 0.19 ng/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠LH,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway
IF: 9.4 -
Inhibition of ferroptosis attenuates oligospermia in male Nrf2 knockout mice
IF: 8.101 -
Tannic acid repair of zearalenone-induced damage by regulating the death receptor and mitochondrial apoptosis signaling pathway in mice
IF: 8.071 -
Ablation of AMPK-Related Kinase MPK38/MELK Leads to Male-Specific Obesity in Aged Mature Adult Mice.
IF: 7.72Journal:DIABETES -
TNFAIP3 Reduction-of-Function Drives Female Infertility and CNS Inflammation.
IF: 7.561Journal:Frontiers in Immunology -
Exposure to hyperandrogen drives ovarian dysfunction and fibrosis by activating the NLRP3 inflammasome in mice
IF: 6.551Journal:SCIENCE OF THE TOTAL ENVIRONMENT -
Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway
IF: 6.53 -
Role of PCSK9 in lipid metabolic disorders and ovarian dysfunction in polycystic ovary syndrome
IF: 6.513 -
Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway
IF: 6.4 -
In utero exposure to acetaminophen and ibuprofen leads to intergenerational accelerated reproductive aging in female mice
IF: 6.268Journal:Communications Biology