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Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

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  • sandwich-Ab-ELISA-Elabscience
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    产品货号:E-EL-M0049c

    中文名:小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

    别称:DIF, TNF-alpha, TNFA, TNFSF2,TNF,TNFa

    规格:
    • 96T
    • 48T
    数量:
    - +
    价格: ¥2400

    反应性: 小鼠

    检测范围: 31.25~2000 pg/mL

    灵敏度: 18.75 pg/mL

    货期: 3个工作日+顺丰周期

    加入购物车 比较 咨询 说明书

    用途

    该试剂盒用于体外定量检测Mouse 血清,血浆或其他生物体液中天然及部分重组TNF-α浓度

    检测原理

    本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上,实验时样品或标准品中的小鼠TNF-α会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素。抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,TNF-α浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TNF-α的浓度。

    反应类型 夹心法
    规格 96T
    反应时间 3.5h
    反应性 Mouse
    检测方法 Colormetric
    检测范围 31.25—2000 pg/mL
    灵敏度 18.75 pg/mL
    样本体积 100μL
    样本类型 血清,血浆或其他生物体液

    特异性

    可检测样本中的小鼠TNF-α,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average Corrected
    2000 2.449
    2.491
    2.47 2.39
    1000 1.726
    1.752
    1.739 1.659
    500 0.993
    0.991
    0.992 0.912
    250 0.483
    0.509
    0.496 0.416
    125 0.3
    0.282
    0.291 0.211
    62.5 0.196
    0.168
    0.182 0.102
    31.25 0.128
    0.136
    0.132 0.052
    0 0.079
    0.081
    0.08 --

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

    Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    92.50 285.40 722.10 85.50 297.30 763.30
    Standard
    deviation
    6.00 16.60 33.90 4.50 11.90 41.20
    C V (%) 6.49 5.82 4.69 5.26 4.00 5.40

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=5) 94-111 101
    EDTA plasma (n=5) 96-110 102
    Cell culture media (n=5) 88-104 96

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 95-106 89-101 88-102
    Average (%) 101 94 94
    1:4 Range (%) 95-111 81-91 91-105
    Average (%) 103 86 98
    1:8 Range (%) 96-112 84-97 91-106
    Average (%) 103 91 98
    1:16 Range (%) 95-112 85-96 95-106
    Average (%) 103 91 101

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2 支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 2-8℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 2-8℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 2-8℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1. 酶标仪(450nm波长滤光片)
    • 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3. 37℃恒温箱,双蒸水或去离子水
    • 4. 吸水纸

    操作步骤

    1. 对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟

    2. 弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟

    3. 弃掉板内液体,洗板3次

    4. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次

    5. 每孔加入90μL底物溶液,37℃孵育15分钟左右

    6. 每孔加入50μL终止液

    7. 立即在450nm波长下读数,处理数据

    客户发表文献

    1. Effect of Oral T2 Antigen on Chronic Prostatitis/ Chronic Pelvic Pain Syndrome in Mice Model
      IF: 2.939
      Journal: inflammation(2019)
      DOI: 10.1007/s10753-019-01072-4
      PMID: 31429013
      引用产品: E-EL-M0049c 
      样本类型: Serum
      物种: Mouse
    2. Dual-Locking Nanoparticles Disrupt the PD-1/PD-L1 Pathway for Efficient Cancer Immunotherapy
      IF: 25.809
      Journal: Advanced materials(2019)
      DOI: 10.1002/adma.201905751
      PMID: 31709671
      引用产品: E-EL-0162c E-EL-M0048c E-EL-M0049c E-EL-M0042c E-EL-M0726c 
      样本类型: Tissue homogenate
      物种: Mouse
    3. NIR/ROS-Responsive Black Phosphorus QD Vesicles as Immunoadjuvant Carrier for Specific Cancer Photodynamic Immunotherapy
      IF: 15.6
      Journal: Advanced Functional Materials(2019)
      DOI: 10.1002/adfm.201905758
      引用产品: E-EL-M0044c E-EL-M0726c E-EL-M0049c 
      样本类型: Serum
      物种: Mouse
    4. Design, Synthesis and Investigation of the Potential Anti-inflammatory Activity of 7-O-Amide Hesperetin Derivatives
      IF: 3.06
      Journal: Molecules(2019)
      DOI: 10.3390/molecules24203663
      PMID: 31614601
      引用产品: E-EL-M0037c E-EL-M0044c E-EL-M0049c 
      样本类型: Cell culture supernatant
      物种: Mouse
    5. NanoRNP Overcomes Tumor Heterogeneity in Cancer Treatment
      IF: 12.279
      Journal: NANO LETTERS(2019)
      DOI: 10.1021/acs.nanolett.9b02501
      PMID: 31593471
      引用产品: E-EL-M0692c E-EL-M0695c E-EL-M0044c E-EL-M0048c E-EL-M0049c 
      样本类型: Serum
      物种: Mouse
    6. Galactan isolated from Cantharellus cibarius modulates antitumor immune response by converting tumor-associated macrophages toward M1-like phenotype
      IF: 6.044
      Journal: Carbohydrate Polymers(2019)
      DOI: 10.1016/j.carbpol.2019.115295
      PMID: 31582086
      引用产品: E-EL-M0049c 
      样本类型: Cell culture supernatant
      物种: Mouse
    7. Effect of Perillaldehyde on Prophylaxis and Treatment of Vaginal Candidiasis in a Murine Model
      IF: 4.259
      Journal: Frontiers in Microbiology(2019)
      DOI: 10.3389/fmicb.2019.01466
      PMID: 31333606
      引用产品: E-EL-M0047c E-EL-M2446c E-EL-M0049c 
      样本类型: Tissue homogenate
      物种: Mouse
    8. Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice.
      IF: 2.518
      Journal: Biomedical and Environmental Sciences(2018)
      DOI: 10.3967/bes2018.101
      PMID: 30423277
      引用产品: E-EL-M0049c E-EL-M0044c E-EL-M0077c E-EL-M0687c E-EL-M0048c E-EL-M2730c E-EL-M0400c E-EL-M0914c E-EL-0060c E-EL-M0419c E-EL-M0618c E-BC-K035-M E-BC-K020-M 
      样本类型: Plasma,Tissue homogenate
      物种: Mouse
    9. Interferon Regulatory Factor 1 Activates Autophagy to Aggravate Hepatic Ischemia-Reperfusion Injury by Increasing High Mobility Group Box 1 Release.
      IF: 5.5
      Journal: Cellular Physiology and Biochemistry(2018)
      DOI: 10.1159/000491732
      PMID: 30016764
      引用产品: E-EL-M0049c 
      样本类型: Serum
      物种: Mouse
    10. The protective effect of formononetin on cognitive impairment in streptozotocin (STZ)-induced diabetic mice.
      IF: 3.457
      Journal: Biomedicine & Pharmacotherapy(2018)
      DOI: 10.1016/j.biopha.2018.07.063
      PMID: 30119194
      引用产品: E-EL-M0049c E-EL-M0037c E-EL-M0044c 
      样本类型: Serum,Tissue homogenate
      物种: Mouse
    11. The Association Between Inflammation, The Microbiome and Urethane-induced Pulmonary Adenocarcinoma.
      IF: 1.39
      Journal: Oncology Letters(2018)
      DOI: 10.3892/ol.2018.8167
      PMID: 29725395
      引用产品: E-EL-M0838c E-EL-M0049c E-EL-M0037c E-EL-M0044c 
      物种: Mouse
    12. Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines.
      IF: 9.326
      Journal: Bone research(2018)
      DOI: 10.1038/s41413-018-0012-0
      PMID: 29619269
      引用产品: E-EL-M0036c E-EL-M0037c E-EL-M0044c E-EL-M0049c E-EL-M0857c E-EL-M1382c 
      物种: Mouse
    13. Blocking The CD38/cADPR Pathway Plays A Double-edged Role In LPS Stimulated Microglia.
      IF: 3.277
      Journal: Neuroscience(2017)
      DOI: 10.1016/j.neuroscience.2017.08.010
      PMID: 28807785
      引用产品: E-EL-M0049c E-EL-M0044c E-EL-M0037c 
      样本类型: Cell culture supernatant
      物种: Mouse
    14. Wu-tou Decoction Attenuates Neuropathic Pain Via Suppressing Spinal Astrocytic IL-1R1/TRAF6/JNK Signaling.
      IF: 5.168
      Journal: Oncotarget(2017)
      DOI: 10.18632/oncotarget.21638
      PMID: 29190962
      引用产品: E-EL-M0049c E-EL-M0037c E-EL-M0018c 
      样本类型: Tissue homogenate
      物种: Mouse
    15. Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis.
      IF: 5.168
      Journal: Oncotarget(2017)
      DOI: 10.18632/oncotarget.22132
      PMID: 29228739
      引用产品: E-EL-M0049c E-EL-M0048c E-EL-M0043c E-EL-M0044c E-EL-M0046c E-EL-M0047c 
      样本类型: Tissue homogenate
      物种: Mouse
    16. Molecular Mechanisms Of the Analgesic Action Of Wu-tou Decoction On Neuropathic Pain In Mice Revealed Using Microarray And Network Analysis.
      IF: 3.223
      Journal: Acta Pharmacologica Sinica(2017)
      DOI: 10.1038/aps.2017.110
      PMID: 28816231
      引用产品: E-EL-M0037c E-EL-M0049c E-EL-M0018c 
      样本类型: Tissue homogenate
      物种: Mouse
    17. Effects Of Sepsis On The Metabolism Of Sphingomyelin And Cholesterol In Mice With Liver Dysfunction.
      IF: 1.261
      Journal: Experimental and Therapeutic Medicine(2017)
      DOI: 10.3892/etm.2017.5226
      PMID: 29285103
      引用产品: E-EL-M0037c E-EL-M0049c 
      样本类型: Plasma
      物种: Mouse
    18. Wogonin Attenuates Inflammation By Activating PPAR-γ In Alcoholic Liver Disease.
      IF: 2.956
      Journal: International Immunopharmacology(2017)
      DOI: 10.1016/j.intimp.2017.06.013
      PMID: 28646664
      引用产品: E-EL-M0049c E-EL-M0044c 
      样本类型: Serum,Cell culture supernatant
      物种: Mouse
    19. Antitumor And Immunomodulatory Effects Of Ginsenoside Rh2 And Its Octyl Ester Derivative In H22 Tumor-Bearing Mice.
      IF: 3.144
      Journal: Journal of Functional Foods(2017)
      DOI: 10.1016/j.jff.2017.03.013
      引用产品: E-EL-M0042c E-EL-M0049c 
      样本类型: Serum
      物种: Mouse
    20. Purification, Characterization And Bioactivities Of Polysaccharides From Pleurotus Ferulae.
      IF: 3.247
      Journal: Food & Function(2017)
      DOI: 10.1039/c7fo00227k
      PMID: 28436508
      引用产品: E-EL-M0726c E-EL-M0049c 
      样本类型: Cell culture supernatant
      物种: Mouse
    21. Repression Of Toll-Like Receptor-4 By microRNA-149-3p Is Associated With Smoking-Related COPD.
      IF: 3.157
      Journal: International Journal of Chronic Obstructive Pulmonary Disease(2017)
      DOI: 10.2147/COPD.S128031
      PMID: 28260877
      引用产品: E-EL-M0037c E-EL-M0049c 
      样本类型: Cell culture supernatant
      物种: Mouse
    22. Glycyrrhiza Uralensis Water Extract Enhances Dendritic Cell Maturation And Antitumor Efficacy Of HPV Dendritic Cell-Based Vaccine.
      IF: 4.259
      Journal: Scientific Reports(2017)
      DOI: 10.1038/srep43796
      PMID: 28272545
      引用产品: E-EL-M0037c E-EL-M0044c E-EL-M0726c E-EL-M0049c 
      样本类型: Cell culture supernatant
      物种: Mouse
    23. Capparis Spinosa Fruit Ethanol Extracts Exert Different Effects On The Maturation Of Dendritic Cells.
      IF: 2.861
      Journal: Molecules(2017)
      DOI: 10.3390/molecules22010097
      PMID: 28067853
      引用产品: E-EL-M2451c E-EL-M0046c E-EL-M0044c E-EL-M0037c E-EL-M0049c 
      样本类型: Cell culture supernatant
      物种: Mouse
    24. Hydrogen Sulfide Mitigates Kidney Injury In High Fat Diet-Induced Obese Mice.
      IF: 4.593
      Journal: Oxidative Medicine And Cellular Longevity(2016)
      DOI: 10.1155/2016/2715718
      PMID: 27413418
      引用产品: E-EL-M0049c E-EL-M0044c E-EL-M0046c 
      样本类型: Plasma
      物种: Mouse
    25. MiR-146a Modulates Macrophage Polarization By Inhibiting Notch1 Pathway In RAW264. 7 Macrophages.
      IF: 2.956
      Journal: International Immunopharmacology(2016)
      DOI: 10.1016/j.intimp.2016.01.009
      PMID: 26800097
      引用产品: E-EL-M0049c E-EL-M0046c 
      样本类型: Cell culture supernatant
      物种: Mouse
    26. Structural Analysis And Immunoregulation Activity Comparison Of Five Polysaccharides From Angelica Sinensis.
      IF: 4.811
      Journal: Carbohydrate Polymers(2016)
      DOI: 10.1016/j.carbpol.2015.12.050
      PMID: 26876821
      引用产品: E-EL-M0049c E-EL-M0048c E-EL-M0042c E-EL-M0044c 
      样本类型: Cell culture supernatant
      物种: Mouse
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      IF: 4.3
      Journal: International Journal of Nanomedicine(2016)
      DOI: 10.2147/IJN.S104166
      PMID: 27574420
      引用产品: E-EL-M0044c E-EL-M0049c 
      样本类型: Plasma
      物种: Mouse
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      IF: 5.476
      Journal: Biochimica Et Biophysica Acta-Molecular Basis of Disease(2016)
      DOI: 10.1016/j.bbadis.2016.03.002
      PMID: 26964796
      引用产品: E-EL-M0049c E-EL-M0037c 
      样本类型: Cell culture supernatant
      物种: Mouse
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      Journal: Parasitology Research(2016)
      DOI: 10.1007/s00436-016-4951-3
      PMID: 26860840
      引用产品: E-EL-M0048c E-EL-M0049c E-EL-M0043c E-EL-M0046c E-EL-M0726c E-EL-M1040c 
      样本类型: Serum,Tissue homogenate
      物种: Mouse
    30. Dendritic Cells Enhance UHMWPE Wear Particle-Induced Osteoclast Differentiation Of Macrophages.
      IF: 3.076
      Journal: Journal of Biomedical Materials Research Part A(2015)
      DOI: 10.1002/jbm.a.35459
      PMID: 25808788
      引用产品: E-EL-M0049c 
      样本类型: Cell culture supernatant
      物种: Mouse
    31. The Effect Of Maternal Obesity On The Expression And Functionality Of Placental P-Glycoprotein: Implications In The Individualized Transplacental Digoxin Treatment For Fetal Heart Failure.
      IF: 2.759
      Journal: Placenta(2015)
      DOI: 10.1016/j.placenta.2015.08.007
      PMID: 26311557
      引用产品: E-EL-M0037c E-EL-M0049c 
      样本类型: Plasma
      物种: Mouse
    32. Inhibition Of Hepatocyte Apoptosis: An Important Mechanism Of Corn Peptides Attenuating Liver Injury Induced By Ethanol.
      IF: 3.226
      Journal: International Journal of Molecular Sciences(2015)
      DOI: 10.3390/ijms160922062
      PMID: 26378531
      引用产品: E-EL-M0049c E-EL-0162c 
      样本类型: Serum,Tissue homogenate
      物种: Mouse
    33. Sulfated Modification Promotes The Immunomodulatory Bioactivities Of Lyciumbarbarum Polysaccharides In Vitro.
      IF: 1.069
      Journal: International Journal of Clinical and Experimental Medicine(2015)
      PMID: 26884954
      引用产品: E-EL-M0042c E-EL-M0044c E-EL-M0048c E-EL-M0049c 
      样本类型: Cell culture supernatant
      物种: Mouse
    34. PGC-1α Silencing Compounds The Perturbation Of Mitochondrial Function Caused By Mutant SOD1 In Skeletal Muscle Of ALS Mouse Model.
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      Journal: Frontiers in Aging Neuroscience(2015)
      DOI: 10.3389/fnagi.2015.00204
      PMID: 26539112
      引用产品: E-EL-M0049c E-EL-M0037c E-EL-M0044c E-EL-M0007c 
      样本类型: Serum
      物种: Mouse
    35. A Comparative Study Of The Effects Upon LPS Induced Macrophage RAW264.7 Inflammation In Vitro Of The Lipids Of Hippocampus Trimaculatus Leach.
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      Journal: Journal of Oleo Science(2015)
      DOI: 10.5650/jos.ess15190
      PMID: 26582156
      引用产品: E-EL-M0049c E-EL-M0037c E-EL-M0044c 
      样本类型: Cell culture supernatant
      物种: Mouse
    36. Ghrelin Ameliorates Intestinal Barrier Dysfunction In Experimental Colitis By Inhibiting The Activation Of Nuclear Factor-Kappa B.
      IF: 2.466
      Journal: Biochemical & Biophysical Research Communications(2015)
      DOI: 10.1016/j.bbrc.2015.01.083
      PMID: 25634696
      引用产品: E-EL-M0049c E-EL-M0048c E-EL-M0037c E-EL-M0044c E-EL-M0046c E-EL-M0047c 
      样本类型: Tissue homogenate
      物种: Mouse
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      IF: 2.441
      Journal: Metabolic Brain Disease
      DOI: 10.1007/s11011-018-0246-y
      PMID: 29948656
      引用产品: E-EL-M0049c E-EL-M0037c 
      样本类型: Plasma
      物种: Mouse
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    Q

    吴*璠Submitted [ Aug 17 2019 ]

    问: 小鼠的血浆样本不够100ul,可以稀释几倍后再测吗

    回复

    A

    adminSubmitted [ Aug 19 2019 ]

    回答: 您好,方便留一个联系方式吗?我们安排技术和您沟通

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    2019-nCoV 新冠病毒相关研究和检测试剂

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