MS Mouse IL-17A(Interleukin 17A) ELISA Kit
中文名称:MS-小鼠白介素17A(IL-17A)酶联免疫吸附测定试剂盒
别称:
CTLA8、CTLA-8、CTLA8cytotoxic T-lymphocyte-associated serine esterase 8、Cytotoxic T-lymphocyte-associated antigen 8、IL17、IL-17、IL17A、IL-17A、IL-17Acytotoxic T-lymphocyte-associated protein 8、IL-17CTLA-8
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 2.19 pg/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-17A浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-17A抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-17A会与包被抗体结合。后依次加入生物素化的抗小鼠IL-17A抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-17A抗体与结合在包被抗体上的小鼠IL-17A结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-17A浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-17A的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 2.19 pg/mL |
样本体积 | 25μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠IL-17A,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Enforced mesenchymal stem cell tissue colonization counteracts immunopathology
IF: 14.404 -
Gut Microflora Modulates Th17/Treg Cell Differentiation in Experimental Autoimmune Prostatitis via the Short-Chain Fatty Acid Propionate
IF: 8.786 -
Successful Treatment of Vitiligo with Cold Atmospheric Plasma‒Activated Hydrogel
IF: 8.551 -
MicroRNA-377-3p released by mesenchymal stem cell exosomes ameliorates lipopolysaccharide-induced acute lung injury by targeting RPTOR to induce autophagy
IF: 8.469Journal:Cell Death & Disease -
HA/CD44 Regulates the T Helper 1 Cells Differentiation by Activating Annexin A1/Akt/mTOR Signaling to Drive the Pathogenesis of EAP.
IF: 7.561Journal:Frontiers in Immunology -
Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function
IF: 7.31Journal:Oxidative Medicine and Cellular Longevity -
Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis
IF: 7.086Journal:CHEMOSPHERE -
Interleukin-17 upregulation participates in the pathogenesis of heart failure in mice via NF-κB-dependent suppression of SERCA2a and Cav1.2 expression
IF: 6.15 -
Deacetylasperulosidic Acid Ameliorates Pruritus, Immune Imbalance, and Skin Barrier Dysfunction in 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis NC/Nga Mice
IF: 5.924 -
Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice
IF: 5.719
E-EL-M0047 | Mouse IL-17A(Interleukin 17A) ELISA Kit | 96T , 48T , 96T*5 |