MS Mouse VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit
中文名称:MS-小鼠血管内皮细胞生长因子A(VEGF-A)酶联免疫吸附测定试剂盒
别称:
VEGF
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 3.21 pg/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中VEGF-A浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠VEGF-A抗体包被于酶标板上,实验时样品(或标准品)中的小鼠VEGF-A会与包被抗体结合。后依次加入生物素化的抗小鼠VEGF-A抗体和辣根过氧化物酶标记的亲和素,抗小鼠VEGF-A抗体与结合在包被抗体上的小鼠VEGF-A结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,VEGF-A浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中VEGF-A的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 3.21 pg/mL |
样本体积 | 25μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠VEGF-A,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa -targeting guide RNA prevents wet age-related macular degeneration in mice
IF: 25.671 -
Tetrahedral framework nucleic acids promote diabetic wound healing via the Wnt signalling pathway
IF: 8.755 -
Astrocytic VEGFA: An essential mediator in blood–brain-barrier disruption in Parkinson's disease
IF: 7.452Journal:GLIA -
Preparation and in vitro and in vivo evaluation of an isoliquiritigenin-loaded ophthalmic nanoemulsion for the treatment of corneal neovascularization
IF: 6.819 -
Positive‐Feedback Regulation of Subchondral H‐Type Vessel Formation by Chondrocyte Promotes Osteoarthritis Development in Mice
IF: 6.741Journal:JOURNAL OF BONE AND MINERAL RESEARCH -
Integration of transdermal chemistry and network pharmacology to decipher the mechanism of ShexiangZhuifeng analgesic plaster to treat rheumatoid arthritis
IF: 6.656 -
Preclinical Evaluation of Recombinant Human IL15 Protein Fused with Albumin Binding Domain on Anti-PD-L1 Immunotherapy Efficiency and Anti-Tumor Immunity in Colon Cancer and Melanoma
IF: 6.639 -
Sinomenine mitigates collagen-induced arthritis mice by inhibiting angiogenesis
IF: 6.53 -
Sorafenib-loaded nanostructured lipid carriers for topical ocular therapy of corneal neovascularization: development, in-vitro and in vivo study
IF: 6.419Journal:DRUG DELIVERY -
Phillyrin Prevents Neuroinflammation-Induced Blood–Brain Barrier Damage Following Traumatic Brain Injury via Altering Microglial Polarization
IF: 5.811Journal:Frontiers in Pharmacology
E-EL-M1292 | Mouse VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit | 96T , 48T , 96T*5 |