本公司产品仅供体外研究使用,不用于临床诊断

NA/NE(Noradrenaline/Norepinephrine) ELISA Kit

  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
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  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
    产品货号:E-EL-0047c

    中文名:去甲肾上腺素(NA/NE)酶联免疫吸附测定试剂盒

    别称:Norepinephrine, NE, noradrenaline, NA, NAd, norad, 4, 5-β-trihydroxyphenethylamine

    规格:
    • 96T
    • 48T
    • 96T*5
    数量:
    - +
    价格: ¥2400

    反应性: 通用

    检测范围: 0.31~20 ng/mL

    灵敏度: 0.19 ng/mL

    用途

    该试剂盒用于体外定量检测血清、血浆或其他相关生物液体中NA/NE浓度。

    检测原理

    本试剂盒采用竞争ELISA法。用NA/NE抗原包被于酶标板上,实验时样品或标准品中的NA/NE与包被的NA/NE竞争生物素标记的抗NA/NE单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,NA/NE浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中NA/NE的浓度。

    反应类型 竞争法
    规格 96T
    反应时间 2.0h
    反应性 通用
    检测方法 Colormetric
    检测范围 0.31-20 ng/mL
    灵敏度 0.19 ng/mL
    样本体积 50μL
    样本类型 血清、血浆或其他相关生物液体

    特异性

    可检测样本中的NA/NE,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (ng/mL) O.D Average
    20 0.32
    0.342
    0.331
    10 0.444
    0.45
    0.447
    5 0.654
    0.648
    0.651
    2.5 0.956
    0.958
    0.957
    1.25 1.339
    1.309
    1.324
    0.63 1.66
    1.658
    1.659
    0.31 1.9
    1.914
    1.907
    0 2.196
    2.21
    2.203

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

      Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (ng/mL)
    1.10 2.30 9.50 1.00 2.40 9.60
    Standard
    deviation
    0.10 0.10 0.30 0.10 0.10 0.40
    C V (%) 9.09 4.35 3.16 10.00 4.17 4.17

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=8) 88-99 93
    EDTA plasma (n=8) 94-110 102
    Cell culture media (n=8) 89-102 97

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

        Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 89-105 99-112 99-114
    Average (%) 96 105 106
    1:4 Range (%) 87-102 90-101 95-111
    Average (%) 94 96 102
    1:8 Range (%) 86-99 90-103 96-110
    Average (%) 92 97 102
    1:16 Range (%) 92-107 86-98 97-112
    Average (%) 97 93 104

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 2-8℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 2-8℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 2-8℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1.酶标仪(450nm波长滤光片)
    • 2.高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3.37℃恒温箱,双蒸水或去离子水
    • 4.吸水纸

    操作步骤

    1. 对应板孔中加入50μL标准品工作液或样本后,立即每孔加入50μL生物素化抗体工作液,37℃孵育45分钟

    2. 弃掉板内液体,洗板3次

    3. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次

    4. 每孔加入90μL底物溶液,37℃孵育15分钟左右

    5. 每孔加入50μL终止液

    6. 立即在450nm波长下读数,处理数据

    客户发表文献

    1. Light modulates glucose metabolism by a retina-hypothalamus-brown adipose tissue axis
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      Journal: CELL(2023)
      DOI: 10.1016/j.cell.2022.12.024
      PMID: 36669474
      样本类型: Serum
      物种: Mouse
    2. β-adrenoreceptor-triggered PKA activation negatively regulates the innate antiviral response
      IF: 22.096
      Journal: Cellular & Molecular Immunology(2023)
      DOI: 10.1038/s41423-022-00967-x
      PMID: 36600052
      样本类型: serum
      物种: Mouse
    3. Neuronal Induction of Bone-Fat Imbalance through Osteocyte Neuropeptide Y
      IF: 16.806
      Journal: Advanced Science(2021)
      DOI: 10.1002/advs.202100808
      PMID: 34719888
      样本类型: marrow-depleted bone
      物种: Mouse
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      IF: 13.934
      Journal: METABOLISM-CLINICAL AND EXPERIMENTAL(2022)
      DOI: 10.1016/j.metabol.2022.155292
      PMID: 35995281
      样本类型: serum
      物种: Mouse
    5. Spinal cord astrocytes regulate myocardial ischemia–reperfusion injury
      IF: 12.416
      Journal: BASIC RESEARCH IN CARDIOLOGY(2022)
      DOI: 10.1007/s00395-022-00968-x
      样本类型: serum
      物种: Rat
    6. Neuroendocrine stress hormones associated with short-term exposure to nitrogen dioxide and fine particulate matter in individuals with and without chronic obstructive pulmonary disease: A panel study in Beijing, China
      IF: 9.988
      Journal: ENVIRONMENTAL POLLUTION(2022)
      DOI: 10.1016/j.envpol.2022.119822
      PMID: 35870527
      样本类型: serum
      物种: Human
    7. Activation of locus coeruleus-spinal cord noradrenergic neurons alleviates neuropathic pain in mice via reducing neuroinflammation from astrocytes and microglia in spinal dorsal horn
      IF: 8.3219
      Journal: Journal of Neuroinflammation(2022)
      DOI: 10.1186/s12974-022-02489-9
      样本类型: spinal cord tissue
      物种: Mouse
    8. Norepinephrine-stimulated HSCs secrete sFRP1 to promote HCC progression following chronic stress via augmentation of a Wnt16B/β-catenin positive feedback loop
      IF: 7.068
      Journal: JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH(2020)
      DOI: 10.1186/s13046-020-01568-0
      样本类型: Serum,Cell culture supernatant
      物种: Human
    9. Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors
      IF: 6.018
      Journal: Computational and Structural Biotechnology Journal(2020)
      DOI: 10.1016/j.csbj.2020.11.009
      PMID: 33304464
      样本类型: blood
      物种: Human
    10. Influence of Diets Enriched with Flavonoids (Cocoa and Hesperidin) on the Systemic Immunity of Intensively Trained and Exhausted Rats
      IF: 6.064
      Journal: Biomolecules(2022)
      DOI: 10.3390/biom12121893
      样本类型: plasma
      物种: Rat
    11. Protective Effect of a Cocoa-Enriched Diet on Oxidative Stress Induced by Intensive Acute Exercise in Rats
      IF: 6.313
      Journal: Antioxidants(2022)
      DOI: 10.3390/antiox11040753
      PMID: 35453438
      样本类型: plasma
      物种: Rat
    12. Maternal anxiety affects embryo implantation via impairing adrenergic receptor signaling in decidual cells
      IF: 6.548
      Journal: Communications Biology(2022)
      DOI: 10.1038/s42003-022-03694-1
      PMID: 35982177
      样本类型: serum,uteri
      物种: Mouse
    13. Uncoupling protein -1 modulates anxiety-like behavior in a temperature-dependent manner
      IF: 6.709
      Journal: JOURNAL OF NEUROSCIENCE(2022)
      DOI: 10.1523/JNEUROSCI.2509-21.2022
      PMID: 36194650
      样本类型: serum
      物种: Mouse
    14. The Role of the Superior Cervical Sympathetic Ganglion in Ischemia Reperfusion-Induced Acute Kidney Injury in Rats
      IF: 5.093
      Journal: Frontiers in Medicine(2022)
      DOI: 10.3389/fmed.2022.792000
      PMID: 35530034
      样本类型: plasma
      物种: Rat
    15. FGF21 ameliorates the neurocontrol of blood pressure in the high fructose-drinking rats
      IF: 5.228
      Journal: Scientific Reports(2016)
      DOI: 10.1038/srep29582
      样本类型: Serum
      物种: Rat
    16. Effect of TLR4/MyD88/NF-kB axis in paraventricular nucleus on ventricular arrhythmias induced by sympathetic hyperexcitation in post-myocardial infarction rats
      IF: 5.295
      Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE(2022)
      DOI: 10.1111/jcmm.17309
      PMID: 35393774
      样本类型: Plasma,myocardial tissue
      物种: Rat
    17. New insights into the central sympathetic hyperactivity post-myocardial infarction: Roles of METTL3-mediated m6A methylation
      IF: 5.31
      Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE(2022)
      DOI: 10.1111/jcmm.17183
      样本类型: serum,myocardium tissue
      物种: Rat
    18. Efficacy of a novel proprietary dietary supplement (TRI 360TM) on psychological symptoms and stress-related quality of life in adult subjects: A randomized controlled clinical trial
      IF: 5.435
      Journal: Frontiers in Psychiatry(2022)
      DOI: 10.3389/fpsyt.2022.919284
      PMID: 36032243
      样本类型: serum
      物种: Human
    19. Renal Denervation Effects on Myocardial Fibrosis and Ventricular Arrhythmias in Rats with Ischemic Cardiomyopathy
      IF: 5.5
      Journal: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY(2018)
      DOI: 10.1159/000489653
      样本类型: Plasma
      物种: Rat
    20. Adjunctive therapy with statins reduces residual albuminuria/proteinuria and provides further renoprotection by downregulating the angiotensin II–AT1 pathway in hypertensive nephropathy
      IF: 4.085
      Journal: JOURNAL OF HYPERTENSION(2017)
      DOI: 10.1097/HJH.0000000000001325
      样本类型: plasma
      物种: Rat
    21. Integrated Functional Neuroimaging, Monoamine Neurotransmitters, and Behavioral Score on Depressive Tendency in Intensive Care Unit Medical Staffs Induced by Sleep Deprivation After Night Shift Work
      IF: 4.157
      Journal: Frontiers in Psychiatry(2022)
      DOI: 10.3389/fpsyt.2022.848709
      PMID: 35392383
      样本类型: serum
      物种: Human
    22. Impact of ginseng on neurotoxicity induced by cisplatin in rats
      IF: 4.223
      Journal: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH(2021)
      DOI: 10.1007/s11356-021-16403-y
      样本类型: brain
      物种: Rat
    23. Investigating the molecular mechanisms of delirium-like neuropsychiatric disorder induced by electromagnetic pulse based on bioinformatics analysis
      IF: 4.399
      Journal: Molecular Brain(2023)
      DOI: 10.1186/s13041-023-00998-z
      样本类型: prefrontal cortex,hippocampus
      物种: Rat
    24. Atrial Fibrillation in Acute Obstructive Sleep Apnea: Autonomic Nervous Mechanism and Modulation
      IF: 4.425
      Journal: Journal of the American Heart Association(2017)
      DOI: 10.1161/JAHA.117.006264
      样本类型: Plasma
    25. Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy
      IF: 4.652
      Journal: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY(2016)
      DOI: 10.1159/000452561
      样本类型: Plasma,Tissue homogenate
      物种: Rat
    26. The Locus Coeruleus–Norepinephrine System Mediates Empathy for Pain through Selective Up-Regulation of P2X3 Receptor in Dorsal Root Ganglia in Rats
      IF: 3.005
      Journal: Frontiers in Neural Circuits(2017)
      DOI: 10.3389/fncir.2017.00066
      样本类型: Serum
    27. Comparison of high-fat style diet-induced dysregulation of baroreflex control of renal sympathetic nerve activity in intact and ovariectomized female rats: Renal sympathetic nerve activity in high-fat style diet fed intact and ovariectomized female rats
      IF: 3.139
      Journal: EXPERIMENTAL BIOLOGY AND MEDICINE(2020)
      DOI: 10.1177/1535370220915673
      样本类型: kidney
      物种: Rat
    28. High frequency deep transcranial magnetic stimulation acutely increases β-endorphins in obese humans
      IF: 3.179
      Journal: ENDOCRINE(2018)
      DOI: 10.1007/s12020-018-1791-1
      样本类型: blood
      物种: Human
    29. Intranasal Dexmedetomidine on Stress Hormones, Inflammatory Markers, and Postoperative Analgesia after Functional Endoscopic Sinus Surgery
      IF: 3.236
      Journal: MEDIATORS OF INFLAMMATION(2015)
      DOI: 10.1155/2015/939431
      样本类型: Serum
      物种: Human
    30. Nrf1 Knock-Down in the Hypothalamic Paraventricular Nucleus Alleviates Hypertension Through Intervention of Superoxide Production-Removal Balance and Mitochondrial Function
      IF: 3.239
      Journal: Cardiovascular Toxicology(2021)
      DOI: 10.1007/s12012-021-09641-6
      PMID: 33582931
      样本类型: Plasma
      物种: Rat
    31. Astaxanthin Ameliorates Blood Pressure in Salt-Induced Prehypertensive Rats Through ROS/MAPK/NF-κB Pathways in the Hypothalamic Paraventricular Nucleus
      IF: 3.239
      Journal: Cardiovascular Toxicology(2021)
      DOI: 10.1007/s12012-021-09695-6
      PMID: 34537923
      样本类型: plasma
      物种: Rat
    32. Apigenin Improves Hypertension and Cardiac Hypertrophy Through Modulating NADPH Oxidase-Dependent ROS Generation and Cytokines in Hypothalamic Paraventricular Nucleus
      IF: 3.239
      Journal: Cardiovascular Toxicology(2021)
      DOI: 10.1007/s12012-021-09662-1
      PMID: 34076830
      样本类型: plasma
      物种: Rat
    33. Perivascular radiofrequency renal denervation lowers blood pressure and ameliorates cardiorenal fibrosis in spontaneously hypertensive rats
      IF: 3.24
      Journal: PLoS One(2017)
      DOI: 10.1371/journal.pone.0176888
      样本类型: plasma
      物种: Rat
    34. A comprehensive study on the relieving effect of Lilium brownii on the intestinal flora and metabolic disorder in p-chlorphenylalanine induced insomnia rats
      IF: 3.503
      Journal: PHARMACEUTICAL BIOLOGY(2022)
      DOI: 10.1080/13880209.2021.2019283
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      样本类型: hypothalamus tissue
      物种: Rat
    35. Norepinephrine stimulation downregulates the β2‐adrenergic receptor–nitric oxide pathway in human pulmonary artery endothelial cells
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      Journal: JOURNAL OF CELLULAR PHYSIOLOGY(2018)
      DOI: 10.1002/jcp.27057
      样本类型: Plasma
      物种: Human
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      Journal: Molecular Medicine Reports(2020)
      DOI: 10.3892/mmr.2020.11501
      PMID: 33000241
      样本类型: Cell culture supernatant,Tissue homogenate
      物种: Mouse
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      DOI: 10.1016/j.neulet.2019.03.049
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      样本类型: Cell culture supernatant
      物种: Human
    38. Dexmedetomidine attenuates acute stress-induced liver injury in rats by regulating the miR-34a-5p/ROS/JNK/p38 signaling pathway
      IF: 2.196
      Journal: JOURNAL OF TOXICOLOGICAL SCIENCES(2022)
      DOI: 10.2131/jts.47.169
      PMID: 35527005
      样本类型: serum
      物种: Rat
    39. Effects of norepinephrine on colonic tight junction protein expression during heat stress
      IF: 2.447
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      DOI: 10.3892/etm.2021.9865
      PMID: 33747161
      样本类型: Plasma
      物种: Rat
    40. Activation of Paraventricular Melatonin Receptor 2 Mediates Melatonin-Conferred Cardioprotection Against Myocardial Ischemia/Reperfusion Injury
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      DOI: 10.1097/FJC.0000000000000851
      样本类型: plasma
      物种: Mouse
    41. Oxidative stress and neuromodulatory effects of deltamethrin and its combination with insect repellents in rats
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      Journal: ENVIRONMENTAL TOXICOLOGY(2019)
      DOI: 10.1002/tox.22741
      PMID: 30884102
      样本类型: Serum
      物种: Rat
    42. Chronic Blockade of NMDAR Subunit 2A in the Hypothalamic Paraventricular Nucleus Alleviates Hypertension Through Suppression of MEK/ERK/CREB Pathway
      IF: 2.689
      Journal: AMERICAN JOURNAL OF HYPERTENSION(2021)
      DOI: 10.1093/ajh/hpab047
      样本类型: plasma
      物种: Rat
    43. A Novel Swine Model of Spontaneous Hypertension With Sympathetic Hyperactivity Responds Well to Renal Denervation
      IF: 2.689
      Journal: AMERICAN JOURNAL OF HYPERTENSION(2016)
      DOI: 10.1093/ajh/hpv066
      样本类型: Plasma,Urine
      物种: Pig
    44. Selective ablation of the ligament of Marshall reduces ischemia and reperfusion-induced ventricular arrhythmias
      IF: 2.766
      Journal: PLoS One(2018)
      DOI: 10.1371/journal.pone.0203083
      PMID: 30153281
      样本类型: Serum
      物种: Canine
    45. The role of the Nox4-derived ROS-mediated RhoA/Rho kinase pathway in rat hypertension induced by chronic intermittent hypoxia
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      Journal: Sleep and Breathing(2017)
      DOI: 10.1007/s11325-016-1449-2
      样本类型: Serum,Tissue homogenate
      物种: Rat
    46. Selective ablation of the ligament of Marshall attenuates atrial electrical remodeling in a short‐term rapid atrial pacing canine model
      IF: 2.873
      Journal: JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY(2018)
      DOI: 10.1111/jce.13658
      样本类型: Serum
      物种: Canine
    47. Lilium davidii extract alleviates p‑chlorophenylalanine‑induced insomnia in rats through modification of the hypothalamic-related neurotransmitters, melatonin and homeostasis of the hypothalamic-pituitary-adrenal axis
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      Journal: PHARMACEUTICAL BIOLOGY(2020)
      DOI: 10.1080/13880209.2020.1812674
      PMID: 32924742
      样本类型: brain
      物种: Rat
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      Journal: BIOMEDICAL CHROMATOGRAPHY(2022)
      DOI: 10.1002/bmc.5385
      PMID: 35445417
      样本类型: serum
      物种: Rat
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      Journal: Journal of General and Family Medicine(2023)
      DOI: 10.1002/jgf2.606
      样本类型: serum
      物种: Human
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      Journal: PHARMAZIE(2019)
      DOI: 10.1691/ph.2019.9379
      样本类型: plasma
      物种: Human,Mouse
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