NADP+/NADPH Colorimetric Assay Kit (WST-8)
中文名称:NADP+/NADPH 比色法试剂盒(WST-8法)

Price:
Size:
96 T (80 samples)
- 样本类型: 动物组织;细胞
- 检测仪器及波长: 酶标仪(450 nm)
- 研究领域: 氧化应激;代谢疾病;糖脂代谢;铜死亡;双硫死亡
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量:
检测原理 |
测定NADP+和NADPH总量:
葡萄糖-6-磷酸(G6P)在葡萄糖-6-磷酸脱氢酶(G6PDH)的作用下氧化生成6-磷酸葡萄糖酸内酯(6-PG),在这一反应过程中NADP+被还原为NADPH;生成的NADPH在电子耦合试剂1-mPMS的作用下将WST-8还原生成橙黄色的formazan,在450 nm左右有最大吸收峰。反应体系中生成的formazan与样品中NADP+和NADPH的总量呈比例关系。 单独测定NADPH的量: 样本提取后,60℃水浴加热30分钟,样本中的NADP+会分解而只保留NADPH。 NADPH将WST-8还原成formazan,通过比色法确定生成的formazan的量,最终可以单独测定样本中NADPH的量。 测定NADP+以及NADP+/NADPH的比值: 根据前两步检测获得的NADP+和NADPH总量以及NADPH单独的量,即可得到样本中NADP+的量以及NADP+/NADPH的比值。 在上述测定过程中,NAD+和NADH对测定结果无影响。 |
灵敏度 | 0.02 μmol/L |
检测范围 | 0.02-5.0 μmol/L |
精密度 | 批间差:5.5% | 批内差:2.1% |
检测时间 | 1 h 10 min |
检测仪器及波长 | 酶标仪(450 nm) |
保存条件 | 该产品可在-20°C避光条件下保存6个月。 |
有效期 | 6个月 |
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