NFκB-p65 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主: Rabbit
- 反应性: Human;Mouse;Rat
- 应用性: WB;IHC-p
Verified Samples |
Verified Samples in WB:KB,Hela,293T,3T3 Verified Samples in IHC:Human stomach cancer,Mouse kidney |
Dilution | WB 1:500-1:2000, IHC 1:100-1:300 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Synthesized peptide derived from human NFκB-p65 around the non-phosphorylation site of Ser281. |
Abbre | NFκB-p65 |
Synonyms | Avian reticuloendotheliosis viral (v rel) oncogene homolog A;MGC131774;NF kappa B p65delta3;NFKB3;Nuclear Factor NF Kappa B p65 Subunit;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B cells 3;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3;OTTHUMP00000233473;OTTHUMP00000233474;OTTHUMP00000233475;OTTHUMP00000233476;OTTHUMP00000233900;p65;p65 NF kappaB;p65 NFkB;relA;TF65;Transcription factor p65;v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65));V rel avian reticuloendotheliosis viral oncogene homolog A;v rel reticuloendotheliosis viral oncogene homolog A (avian);V rel reticuloendotheliosis viral oncogene homolog A;nuclear factor of kappa light polypeptide gene enhancer in B cells 3;p65抗体 |
Swissprot | |
Calculated MW | 60kDa |
Observed MW | 60kDa |
Cellular Localization | Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalized with RELA in the nucleus upon TNF-alpha induction. |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer; Cell Biology; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism; Microbiology; Neuroscience; Signal Transduction |
Conjugation | Unconjugated |
Background | Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel,are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin κ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity. |
Western Blot analysis of KB, Hela, 293T, 3T3 cells using NFκB-p65 Polyclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Human stomach cancer tissue using NFκB-p65 Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Mouse kidney tissue using NFκB-p65 Polyclonal Antibody at dilution of 1:200.
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