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Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit

  • 中文名称:游离脂肪酸(NEFA/FFA)比色法测试盒

  • 检测仪器及波长:酶标仪(690-730 nm,最佳波长715 nm)

订购 E-BC-K013-M

规格:
  • 48T(16 samples)
  • 96T(40 samples)
价格: ¥480
数量:

说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证

根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算

生化试剂盒数量计算器x

需测样本数:

单个样本测量孔数:

试剂盒测样本孔数:

  • 16
  • 18
  • 19
  • 22
  • 23
  • 24
  • 25
  • 32
  • 36
  • 40
  • 42
  • 43
  • 44
  • 46
  • 48
  • 50
  • 80
  • 86
  • 92
  • 96
  • 100
  • 480

x

计算

所需盒子量:

检测原理

在弱酸性条件下,游离脂肪酸与铜盐反应生成铜皂,在715 nm处有特征吸收峰,在一定范围内游离脂肪酸含量与显色程度呈线性关系,可计算出游离脂肪酸的含量。

关键信息

指标简称 NEFA/FFA
样本类型 动物组织
灵敏度 0.15 mmol/L
检测范围 0.15-1.5 mmol/L
精密度 批间差:5.1%  |  批内差:3.3%
检测仪器及波长 酶标仪(690-730 nm,最佳波长715 nm)
保存条件 2-8℃
有效期 6个月

文献

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Diabetes/Metabolism Research and Reviews (2017) IF: 3.263
    Gut Microbiota After Roux-En-Y Gastric Bypass And Sleeve Gastrectomy In A Diabetic Rat Model: Increased Diversity And Associations Of Discriminant Genera With Metabolic Changes.

    DOI: 10.1002/dmrr.2857

    PMID: 27572277

    Sample: Serum
  2. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 3.743
    2,3,5,4'-tetrahydroxystilbence-2-O-β-D-glucoside attenuates hepatic steatosis via IKKβ/NF-κB and Keap1-Nrf2 pathways in larval zebrafish

    DOI: 10.1016/j.biopha.2020.110138

    PMID: 99999999

    Sample: Tissue homogenate
  3. IF: 3.304
    Inhibition of adiposity and related metabolic disturbances by polyphenol-rich extract of Boswellia serrata gum through alteration of adipo/cytokine profiles

    DOI: 10.1007/s10787-018-0519-4

    PMID: 99999999

    Sample: Cell lysate
  4. Saudi Journal of Biological Sciences (2020) IF: 2.802
    Anti-obesity potential of Moringa olifera seed extract and lycopene on high fat diet induced obesity in male Sprauge Dawely rats

    DOI: 10.1016/j.sjbs.2020.06.026

    PMID: 32994733

    Sample: Serum
  5. Heliyon (2020)
    Combination of Pancreastatin inhibitor PSTi8 with metformin inhibits Fetuin-A in type 2 diabetic mice

    DOI: 10.1016/j.heliyon.2020.e05133

    PMID: 33033766

    Sample: Plasma,Tissue homogenate
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Reviews/Q&A

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  • 评论
  • 问答
Q

吴*兰Submitted [ Nov 09 2022 ]

问: 这个是否有检测血浆的方法呢,比如抽提时间是否也是一样,样本量大概需要多少呢

回复

A

adminSubmitted [ Nov 10 2022 ]

回答: 这个试剂盒不适用于血浆样本,检测血浆样本推荐货号为E-BC-K013-S的试剂盒,处理方法参考该说明书中血清样本。

A

adminSubmitted [ Nov 10 2022 ]

回答: 

Q

高*鹏Submitted [ Oct 17 2022 ]

问: 用这个制备匀浆样品以后要做的低温震荡抽提大概是用怎样的频率呢

回复

A

adminSubmitted [ Oct 18 2022 ]

回答: 低温震荡器中震荡抽提2h,震荡的频率没有具体要求,不快也不慢就可以。

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