One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488)
中文名称：一步法TUNEL流式凋亡检测试剂盒（绿色，Elab Fluor® 488）
Price:
Size:
100Assays
50Assays
20Assays
- 应用： 细胞凋亡-DNA片段化
| 检测原理 | 细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过流式细胞仪进行检测。 |
| 检测方法 | 荧光;流式 |
| 样本类型 | 细胞 |
| 检测时间 | 2.5-3 hours |
| 检测仪器 | 流式细胞仪 |
| 荧光 | Elab Fluor®488 |
| Ex/Em | 495/520 |
| Channel set | FITC |
| 自备试剂 | PBS(含1%BSA)缓冲液(pH7.2～7.4) |
| 保存温度 | 该产品可在-20°C避光条件下保存12个月。 |
| 有效期 | 12个月 |
| 运输条件 | 冰袋 |
Camptothecin-induced apoptosis in HL-60 cells. Test: HL-60 cells were treated with 2.5 μM Camptothecin for 4h. Control: Normal HL-60 cells were not treated with drugs. Negative: HL-60 cells were treated with 2.5 μM camptothecin for 4h without TdT enzyme.
Flow cytometry analysis of camptothecin-induced apoptosis in Jarket cells.
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TFEB attenuates hyperglycemia-induced retinal capillary endothelial cells injury via autophagy regulation
IF: 4.473Journal：CELL BIOLOGY INTERNATIONAL
| E-CK-A237 | Annexin V-Elab Fluor® 488/PI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
| E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100Assays , 50Assays , 20Assays |
| E-CK-A321 | One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488) | 100Assays , 50Assays , 20Assays |
| E-CK-A362 | Enhanced Cell Counting Kit 8 (WST-8/CCK8) | 500Tests , 100Tests , 500Tests×20 |
| E-CK-A425 | One-step TUNEL Flow Cytometry Apoptosis Kit (Red, Elab Fluor® 555) | 100Assays , 50Assays , 20Assays |
Q1:What is the difference between TUNEL in-situ kit and TUNEL flow cytometry kit?
The main difference lies in the detection methods, for TUNEL in-situ kits, a fluorescence microscope is used for observing the results, as for TUNEL flow ytometry kit, the sample will be detected by a flow cytometer. On the other hand, TUNEL in-situ kit is more suitable for tissue sections and cell slides or smears, while TUNEL flow ytometry kit is more suitable for suspended cells and adherent cells.
