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热门搜索: 最新活动 文献引用 新品速递 一步法ELISA 流式抗体 铜死亡 Annexin V凋亡检测 TUNEL凋亡检测 未包被ELISA 铁死亡 双硫死亡

One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

中文名称:一步法TUNEL原位细胞凋亡检测试剂盒(绿色,FITC)
One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
  • One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
  • One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
  • One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
  • One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
  • +2

Price: ¥3600.00 ¥2000.00 ¥1500.00

Size:
100Assays 50Assays 20Assays
  • 应用: 细胞凋亡-DNA片段化
检测原理
细胞在发生凋亡时,会激活一些特异性的DNA内切酶,这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段,在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶(脱氧核糖核酸末端转移酶)将标记的dUTP连接到断裂DNA暴露的3'-OH末端,通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞,从而可以通过荧光显微镜进行检测。
检测方法
荧光法
样本类型
石蜡切片;冰冻切片;细胞爬片;细胞涂片
检测时间
3 hours
检测仪器
荧光显微镜
荧光
FITC
Ex/Em
490/520
Channel set
FITC
Filter set
FITC
自备试剂
4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇
保存温度
该产品可在-20°C避光条件下保存12个月。
有效期
12个月
运输条件
冰袋
Paraffin embedded rat heart was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope.
Paraffin embedded mouse liver was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope
Paraffin embedded mouse spleen was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscop
Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.
Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.
E-CK-A211 Annexin V-FITC/PI Apoptosis Kit 200Assays , 100Assays , 50Assays , 20Assays
E-CK-A301 Mitochondrial Membrane Potential Assay Kit (with JC-1) 100Assays , 50Assays , 20Assays
E-CK-A321 One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488) 100Assays , 50Assays , 20Assays
E-CK-A362 Enhanced Cell Counting Kit 8 (WST-8/CCK8) 500Tests , 100Tests , 500Tests×20


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