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One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

怎么选择合适的TUNEL 凋亡检测试剂盒

文献(30)

  • 中文名称:一步法TUNEL原位细胞凋亡检测试剂盒（绿色，FITC）

  • 货号:E-CK-A320

Applications:

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规格:
  • 20Assays
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价格: ￥1500
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检测原理

细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过荧光显微镜进行检测。

基本信息

应用类型 细胞凋亡-DNA片段化
检测方法 荧光
样本类型 石蜡切片，冰冻切片，细胞爬片，细胞涂片
检测时间 3 hours
检测仪器 荧光显微镜
荧光 FITC
Ex/Em (nm) 490/520
Channel set FITC
Filter set FITC
自备试剂 4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇
保存温度 -20°C, shading light
运输条件 冰袋
保质期 12 months

操作指南

文献

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