PCNA Polyclonal Antibody

Verified Samples in WB:Hela,HepG2,MCF-7,Human live,Rat cancer
Verified Samples in IHC:Human lung cancer,Mouse spleen,Rat lung

WB 1:500-1:2000, IHC 1:500-1:1000

Polyclonal

IgG

Recombinant protein corresponding to Mouse PCNA

Proliferating Cell Nuclear Antigen

ATLD2;cb16;Cyclin;DNA polymerase delta auxiliary protein;etID36690.10;fa28e03;fb36g03;HGCN8729;MGC8367;Mutagen-sensitive 209 protein;OTTHUMP00000030189;OTTHUMP00000030190;PCNA;Pcna/cyclin;PCNA;PCNAR;Polymerase delta accessory protein;Proliferating cell nuclear antigen;wu:fa28e03;wu:fb36g03抗体

36kDa

36kDa

Nucleus

470 μg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4

Affinity purification

Cancer; Cell Biology; Epigenetics and Nuclear Signaling; Tags and Cell Markers

Unconjugated

Proliferating Cell Nuclear Antigen,commonly known as PCNA,is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells.