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PE Anti-Mouse CD86 Antibody[GL-1]

Price: ¥1800 ¥720

Size:
100μg 25μg
  • 应用性: FCM
  • 亚型: Rat IgG2a, κ
  • 宿主: Rat
Background
CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
Alternate Names
T-lymphocyte activation antigen CD86;Cd86;Activation B7-2 antigen;Early T-cell costimulatory molecule 1;ETC-1;
Swissprot
Gene ID
Clone No
Host
Rat
Reactivity
Mouse
Application
Isotype
Rat IgG2a, κ
Isotype Control
Conjugation
PE
Conjugation Information
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
Spectrum
Storage Buffer
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
Storage
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Expiration date
12个月
Shipping
冰袋
LPS-stimulated (3 days) C57BL/6 murine splenocytes are stained with PE Anti-Mouse CD86 Antibody (filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.
E-AB-F09940 AF/LE Purified Anti-Mouse CD86 Antibody[GL-1] 500μg , 50μg , 1mg
E-AB-F0994A Purified Anti-Mouse CD86 Antibody[GL-1] 100μg , 25μg
E-AB-F0994B Biotin Anti-Mouse CD86 Antibody[GL-1] 100μg , 25μg
E-AB-F0994C FITC Anti-Mouse CD86 Antibody[GL-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0994D PE Anti-Mouse CD86 Antibody[GL-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0994E APC Anti-Mouse CD86 Antibody[GL-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0994H PE/Cyanine7 Anti-Mouse CD86 Antibody[GL-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0994I PE/Cyanine5.5 Anti-Mouse CD86 Antibody[GL-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0994L Elab Fluor® 488 Anti-Mouse CD86 Antibody[GL-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0994M Elab Fluor® 647 Anti-Mouse CD86 Antibody[GL-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0994P PE/Elab Fluor® 594 Anti-Mouse CD86 Antibody[GL-1] 100Tests , 50Tests , 100Tests×2
E-AB-F0994UC FITC Anti-Mouse CD86 Antibody[GL-1] 100μg , 25μg
E-AB-F0994UE APC Anti-Mouse CD86 Antibody[GL-1] 100μg , 25μg
E-AB-F0994UH PE/Cyanine7 Anti-Mouse CD86 Antibody[GL-1] 100μg , 25μg
E-AB-F0994UI PE/Cyanine5.5 Anti-Mouse CD86 Antibody[GL-1] 100μg , 25μg
E-AB-F0994UL Elab Fluor® 488 Anti-Mouse CD86 Antibody[GL-1] 100μg , 25μg
E-AB-F0994UM Elab Fluor® 647 Anti-Mouse CD86 Antibody[GL-1] 100μg , 25μg
E-AB-F0994UP PE/Elab Fluor® 594 Anti-Mouse CD86 Antibody[GL-1] 100μg , 25μg

Q1:What are the recommendations for flow cytometry of mouse macrophages to detect M1 and M2 type macrophages?

As a recommandation, F4/80 and CD11b are always used for identyfing macrophages of mouse, and CD86 and CD206 can be used for a further seperation for M1 abd M2 respectively. Specific indicators can be determined according to the literature and experimental requirements.



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