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PE/Cyanine5.5 Anti-Human CD86 Antibody[BU63]

Price: ¥3120 ¥1200 ¥4680

Size:
100Tests 20Tests 100Tests×2
  • 应用性: FCM
  • 亚型: Mouse IgG1, κ
  • 宿主: Mouse
Background
CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.
Alternate Names
T-lymphocyte activation antigen CD86;Cd86;Activation B7-2 antigen;Early T-cell costimulatory molecule 1;ETC-1;
Swissprot
Gene ID
Clone No
Host
Mouse
Reactivity
Human
Application
Isotype
Mouse IgG1, κ
Isotype Control
Conjugation
PE/Cyanine 5.5
Conjugation Information
PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter).
Spectrum
Storage Buffer
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
Storage
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Expiration date
12个月
Shipping
冰袋
Human peripheral blood monocytes are stained with FITC Anti-Human CD14 Antibody and PE/Cyanine5.5 Anti-Human CD86 Antibody[BU63] (Left). Monocytes are stained with FITC Anti-Human CD14 Antibody and PE/Cyanine5.5 Mouse IgG1, κ Isotype Control (Right).
E-AB-F1012A Purified Anti-Human CD86 Antibody[BU63] 100μg , 25μg
E-AB-F1012B Biotin Anti-Human CD86 Antibody[BU63] 100μg , 25μg
E-AB-F1012C FITC Anti-Human CD86 Antibody[BU63] 100Tests , 20Tests , 100Tests×2
E-AB-F1012D PE Anti-Human CD86 Antibody[BU63] 100Tests , 20Tests , 100Tests×2
E-AB-F1012E APC Anti-Human CD86 Antibody[BU63] 100Tests , 20Tests , 100Tests×2
E-AB-F1012G PE/Cyanine5 Anti-Human CD86 Antibody[BU63] 100Tests , 20Tests , 100Tests×2
E-AB-F1012H PE/Cyanine7 Anti-Human CD86 Antibody[BU63] 100Tests , 20Tests , 100Tests×2
E-AB-F1012J PerCP/Cyanine5.5 Anti-Human CD86 Antibody[BU63] 100Tests , 20Tests , 100Tests×2
E-AB-F1012L Elab Fluor® 488 Anti-Human CD86 Antibody[BU63] 100Tests , 20Tests , 100Tests×2
E-AB-F1012M Elab Fluor® 647 Anti-Human CD86 Antibody[BU63] 100Tests , 20Tests , 100Tests×2
E-AB-F1012P PE/Elab Fluor® 594 Anti-Human CD86 Antibody[BU63] 100Tests , 20Tests , 100Tests×2
E-AB-F1012S Elab Fluor® Red 780 Anti-Human CD86 Antibody[BU63] 100Tests , 20Tests , 100Tests×2

Q1:What are the recommendations for flow cytometry of mouse macrophages to detect M1 and M2 type macrophages?

As a recommandation, F4/80 and CD11b are always used for identyfing macrophages of mouse, and CD86 and CD206 can be used for a further seperation for M1 abd M2 respectively. Specific indicators can be determined according to the literature and experimental requirements.



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