PE/Cyanine5.5 Anti-Human CD86 Antibody[BU63]
Price:
Size:
100Tests
20Tests
100Tests×2
- 应用性: FCM
- 亚型: Mouse IgG1, κ
- 宿主: Mouse
Background | CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells. |
Alternate Names | T-lymphocyte activation antigen CD86;Cd86;Activation B7-2 antigen;Early T-cell costimulatory molecule 1;ETC-1; |
Swissprot | |
Gene ID | |
Clone No | |
Host | Mouse |
Reactivity | Human |
Application | |
Isotype | Mouse IgG1, κ |
Isotype Control | |
Conjugation | PE/Cyanine 5.5 |
Conjugation Information | PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter). |
Spectrum | |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA. |
Storage | This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze. |
Expiration date | 12个月 |
Shipping |
冰袋 |
Human peripheral blood monocytes are stained with FITC Anti-Human CD14 Antibody and PE/Cyanine5.5 Anti-Human CD86 Antibody[BU63] (Left). Monocytes are stained with FITC Anti-Human CD14 Antibody and PE/Cyanine5.5 Mouse IgG1, κ Isotype Control (Right).
E-AB-F1012A | Purified Anti-Human CD86 Antibody[BU63] | 100μg , 25μg |
E-AB-F1012B | Biotin Anti-Human CD86 Antibody[BU63] | 100μg , 25μg |
E-AB-F1012C | FITC Anti-Human CD86 Antibody[BU63] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1012D | PE Anti-Human CD86 Antibody[BU63] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1012E | APC Anti-Human CD86 Antibody[BU63] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1012G | PE/Cyanine5 Anti-Human CD86 Antibody[BU63] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1012H | PE/Cyanine7 Anti-Human CD86 Antibody[BU63] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1012J | PerCP/Cyanine5.5 Anti-Human CD86 Antibody[BU63] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1012L | Elab Fluor® 488 Anti-Human CD86 Antibody[BU63] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1012M | Elab Fluor® 647 Anti-Human CD86 Antibody[BU63] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1012P | PE/Elab Fluor® 594 Anti-Human CD86 Antibody[BU63] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1012S | Elab Fluor® Red 780 Anti-Human CD86 Antibody[BU63] | 100Tests , 20Tests , 100Tests×2 |
Q1:What are the recommendations for flow cytometry of mouse macrophages to detect M1 and M2 type macrophages?
As a recommandation, F4/80 and CD11b are always used for identyfing macrophages of mouse, and CD86 and CD206 can be used for a further seperation for M1 abd M2 respectively. Specific indicators can be determined according to the literature and experimental requirements.