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PE/Cyanine5 Anti-Human CD62L Antibody[HI62L]

Uniprot : P14151

货号: E-AB-F1336G
应用: FCM
Isotype: Mouse IgG2a, κ
宿主: Mouse

Applications:

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20Tests
100Tests
100Tests×2

价格: ￥960

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Antigen Details

Alternate Names	L-selectin,Sell,CD62 antigen-like family member L,LAM-1,LECAM1,Lymph node homing receptor,Ly-22, CD62L,Lnhr,Ly22
Swissprot	P14151
Gene ID	6402
References	1. Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. 2. Kishimoto T, et al. 1991. Blood 78:805.

Product Details

Form	Liquid
Clone No.	HI62L (See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2a, κ
Isotype Control	PE/Cyanine5 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802G]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine5
	PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).

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Background

CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.