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PGE2(Prostaglandin E2) ELISA Kit

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  • competitive-ELISA-Elabscience
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  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
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  • competitive-ELISA-Elabscience
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    产品货号:E-EL-0034c

    中文名:前列腺素E2(PGE2)酶联免疫吸附测定试剂盒

    别称:PG-E2, Dinoprostone

    规格:
    • 96T
    • 48T
    • 96T*5
    数量:
    - +
    价格: ¥2400

    检测范围: 31.25~2000 pg/mL

    灵敏度: 18.75 pg/mL

    货期: 3个工作日+顺丰周期

    在线咨询 比较 加入购物车 说明书

    用途

    该试剂盒用于体外定量检测血清、血浆或其他相关生物液体中天然及部分重组PGE2浓度。

    检测原理

    本试剂盒采用竞争ELISA法。用PGE2抗原包被于酶标板上,实验时样品或标准品中的PGE2与包被的PGE2竞争生物素标记的抗PGE2单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,PGE2浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中PGE2的浓度。

    反应类型 竞争法
    规格 96T
    反应时间 2.0h
    反应性
    检测方法 Colormetric
    检测范围 31.25-2000 pg/mL
    灵敏度 18.75 pg/mL
    样本体积 50μL
    样本类型 血清、血浆或其他相关生物液体

    特异性

    可检测样本中的PGE2,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average
    2000 0.381
    0.393
    0.387
    1000 0.489
    0.517
    0.503
    500 0.702
    0.696
    0.699
    250 0.974
    1.002
    0.988
    125 1.341
    1.331
    1.336
    62.5 1.674
    1.662
    1.668
    31.25 1.913
    1.925
    1.919
    0 2.261
    2.269
    2.265

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

      Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    101.30 236.80 779.30 96.00 259.90 728.60
    Standard
    deviation
    6.60 11.60 42.10 4.90 13.80 31.30
    C V (%) 6.52 4.90 5.40 5.10 5.31 4.30

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=5) 92-109 99
    EDTA plasma (n=5) 87-99 94
    Cell culture media (n=5) 86-96 91

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

        Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 95-110 91-104 89-101
    Average (%) 103 98 96
    1:4 Range (%) 85-95 89-101 101-117
    Average (%) 90 95 107
    1:8 Range (%) 87-100 92-104 96-111
    Average (%) 92 97 102
    1:16 Range (%) 88-104 93-105 99-114
    Average (%) 95 98 106

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 2-8℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 2-8℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 2-8℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1.酶标仪(450nm波长滤光片)
    • 2.高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3.37℃恒温箱,双蒸水或去离子水
    • 4.吸水纸

    操作步骤

    1. 对应板孔中加入50μL标准品工作液或样本后,立即每孔加入50μL生物素化抗体工作液,37℃孵育45分钟

    2. 弃掉板内液体,洗板3次

    3. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次

    4. 每孔加入90μL底物溶液,37℃孵育15分钟左右

    5. 每孔加入50μL终止液

    6. 立即在450nm波长下读数,处理数据

    客户发表文献

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      DOI: 10.1136/jitc-2019-000489
      PMID: 32269145
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      样本类型: Cell sample
      物种: Human
    2. Effects of Tarantula cubensis alcoholic extract and Nerium oleander distillate on experimentally induced colon cancer
      IF: 5.413
      Journal: development(2019)
      DOI: 99999999
      PMID: 99999999
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      样本类型: Serum
      物种: Rat
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      Journal: Journal of Crohn's & Colitis(2015)
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      样本类型: Plasma
      物种: Rat
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      IF: 4.018
      Journal: Journal of Reproductive Immunology(2020)
      DOI: 10.1016/j.jri.2020.103192
      PMID: 32950783
      引用产品: E-EL-0034c E-EL-H0109c E-EL-H1388c E-EL-H1564c 
      样本类型: Cell culture supernatant
      物种: Human
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      DOI: 10.1021/acs.jafc.0c03123
      PMID: 32797754
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      样本类型: Cell culture medium
      物种: Human
    6. Gastroprotective effect of gallic acid against ethanol-induced gastric ulcer in rats: Involvement of the Nrf2/HO-1 signaling and anti-apoptosis role
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      Journal: Biomedicine & Pharmacotherapy(2020)
      DOI: 10.1016/j.biopha.2020.110075
      PMID: 32179202
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      样本类型: Serum,Tissue homogenate
      物种: Rat
    7. A study of nanometre aggregates formation mechanism and antipyretic effect in Bai-Hu-Tang, an ancient Chinese herbal decoction
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      Journal: Biomedicine & Pharmacotherapy(2020)
      DOI: 10.1016/j.biopha.2020.109826
      PMID: 31978766
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      样本类型: Tissue homogenate
      物种: Rat
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      DOI: 10.1016/j.biopha.2020.110471
      PMID: 99999999
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      样本类型: Serum
      物种: Rat
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      Journal: Frontiers in Pharmacology(2019)
      DOI: 10.3389/fphar.2019.00836
      PMID: 31440159
      引用产品: E-EL-0034c 
      样本类型: Cell culture supernatant
      物种: Human,Mouse
    10. In utero exposure to DBP stimulates release of GnRH by increasing the secretion of PGE2 in the astrocytes of the hypothalamus in the offspring mice
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      Journal: Ecotoxicology and Environmental Safety(2020)
      DOI: 10.1016/j.ecoenv.2020.110698
      PMID: 32388187
      引用产品: E-EL-0034c E-EL-0071c 
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      Journal: Science of The Total Environment(2019)
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      PMID: 30833257
      引用产品: E-EL-0034c E-EL-0066c E-EL-0071c E-EL-0155c 
      样本类型: Plasma
      物种: Lizard
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      IF: 3.238
      Journal: Inflammopharmacology(2020)
      DOI: 10.1007/s10787-019-00596-3
      PMID: 31037575
      引用产品: E-EL-0034c E-EL-R0019c 
      样本类型: Serum
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    13. Cisplatin induces chemoresistance through the PTGS2-mediated anti-apoptosis in gastric cancer
      IF: 3.673
      Journal: The International Journal of Biochemistry & Cell Biology(2019)
      DOI: 10.1016/j.biocel.2019.105610
      PMID: 31518663
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      样本类型: Cell culture supernatant
      物种: Human
    14. Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways
      IF: 3.61
      Journal: Phytomedicine(2018)
      DOI: 10.1016/j.phymed.2018.11.015
      PMID: 31005814
      引用产品: E-EL-0034c E-EL-H0102c E-EL-H0134c 
      样本类型: Cell culture supernatant
      物种: Human
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      IF: 2.762
      Journal: Plants(2020)
      DOI: 10.3390/plants9091127
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      样本类型: Cell culture supernatant
      物种: Mouse
    16. Differential effects of performance-enhancing drugs 'Methamphetamine' and 'hCG' on ex-vivo cultured primary blood mononuclear cells of male athletes
      IF: 2.754
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      DOI: 10.1007/s43440-020-00066-6
      PMID: 32124391
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    17. LL-37 alone and in combination with IL17A enhances proinflammatory cytokine expression in parallel with hyaluronan metabolism in human synovial sarcoma cell line SW982—A step toward understanding the development of inflammatory arthritis
      IF: 2.74
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      DOI: 10.1371/journal.pone.0218736
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    18. Synthesis and Evaluation of Novel D-ring Substituted Steroidal Pyrazolines as Potential Anti-inflammatory Agents
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      DOI: 10.1016/j.steroids.2019.03.012
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      Journal: Biological trace element research(2018)
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      物种: Rat
    20. Galangin Suppresses Renal Inflammation via the Inhibition of NF-κB, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells
      IF: 2.197
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      PMID: 31240210
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      DOI: 10.2147/JPR.S125805
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    23. Role of ascitic prostaglandin E2 in diagnosis of spontaneous bacterial peritonitis and prediction of in-hospital mortality in patients with decompensated cirrhosis
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      DOI: 10.1097/MD.0000000000016016
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    24. Gentiopicroside protects neurons from astrocyte-mediated inflammatory injuries by inhibition of nuclear factor-κB and mitogen-activated protein kinase signaling pathways
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      样本类型: Serum
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      DOI: 10.1142/S0192415X20500214
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      样本类型: Serum
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      样本类型: Serum
      物种: Rat
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