Phospho-AMPK alpha1/2 (Thr183/172) Polyclonal Antibody

Verified Samples in WB:L929,Rat kidney,Mouse brain
Verified Samples in IHC:Mouse brain
Verified Samples in IF:Rat heart

WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200

Polyclonal

IgG

Synthesized peptide derived from human AMPKα1/2 around the phosphorylation site of Thr183/172

AMPKα1/2 (phospho Thr183/172)

PRKAA1;AMPK1;5'-AMP-activated protein kinase catalytic subunit alpha-1;AMPK subunit alpha-1;Acetyl-CoA carboxylase kinase;ACACA kinase;Hydroxymethylglutaryl-CoA reductase kinase;HMGCR kinase;Tau-protein kinase PRKAA1;PRKAA2;AMPK;抗体

62kDa

63kDa

Cytosol,Nucleus,nuclear speck,nucleoplasm,nucleus,Plasma Membrane,apical plasma membrane,Other locations:cytoplasm,intracellular,nucleotide-activated protein kinase complex

1 mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Affinity purification

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal Transduction

Unconjugated

AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.